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Investigation of the Relationship Between Blood Lipid Peroxidation and the Prevalence of Aflatoxin M1 in Milk Samples from Mothers and Cows Living in Kars and Surrounding Villages

Year 2020, Volume: 13 Issue: 2, 67 - 75, 31.12.2020

Abstract

Aflatoxins are highly toxic secondary metabolites produced by molds. They cause various deformations in living systems. One of these is lipid peroxidation in mammals. Aflatoxins in milk are one of the most important factors that can increase lipid peroxidation in living life. Infants and children fed with milk and dairy products containing aflatoxin M1 (AMF1) have a greater effect.
In this study, aflatoxin levels were determined in 80 healthy cows and 80 breast milk samples from the same region, and the relationship between feeding patterns of mothers fed with these milk and their effects on blood lipid peroxidation were tried to be determined. Immunoassay kit (Ridascreen, Riedel-de Haen cat no. R1121 R-Biopharm GmbH, Darmstadt, Germany), a competitive enzyme, was used to determine AFM1 levels. Lipid peroxidation levels and AFM1 correlations were demonstrated in blood samples of mothers infected with AFM1. Samples were analyzed in 4 stages (collecting literature material, experimental applications, statistical analysis and report writing stage) in 24 months.
According to the results obtained in this study, it can be said that good agricultural practices should be adopted in order to maintain and maintain official control and implementation mechanisms at all stages from milk production to consumption, where aflatoxins in milk and dairy products are one of the biggest threats to public health

Supporting Institution

Kafkas Üniversitesi

Project Number

FEM15

References

  • 1. Jamali M., Ebrahimi M.A., Karimipour M, et al. (2012). An insight into the distribution, genetic diversity, and mycotoxin production of Aspergillus section Flavi in soils of pistachio orchards. Folia Microbiologica, 57(1), 27-36.
  • 2. Bennett JW. (2010). An overview of the genus Aspergillus, Caiser Academic Press, Portland, ISBN: 978-1-904455-53-0.
  • 3. Iqbal S.Z, Asi M.R, & Ariñ, A. (2013). Aflatoxins. In S. Maloy & K. Hughes (Eds.), Brenner's Encyclopedia of Genetics, USA: Institute of Molecular Scieces. (2 ed., pp. 43–47).
  • 4. Coppock RW, Christian R G, & Jacobsen BJ. (2012). Chapter 88- Aflatoxins. In R. C. Gupta (Ed.), Veterinary Toxicology (Second Edition), Boston: Academic Press. (pp. 1181-1199). 5. Kalantari H, & Kalantari G. (2007). Evaluation Of Aflatoxins Contamination In Baby Food Supplements (Mamana & Ghoncheh). Jundushapur Journal of Natural Pharmaceutical Products. 6(1), 42-50.
  • 6. Pitt, J I. (2014). Mycotoxins: Aflatoxins. In Y. Motarjemi (Ed.), Encyclopedia of Food Safety, 289-294) Waltham: Academic Press.
  • 7. Kamkar A. (2008). Detection of aflatoxin M1 in UHT milk sample by ELISA. J Vet Res. 63(2), 7-12.
  • 8. Mulunda M, Bakunzi F, Motsei L et al. (2013). A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review. In H. A. Makun (Ed.), Mycotoxin and Food Safety in Developing Countries. 39-60. Croatia: INTECH Open Access Publisher.
  • 9. Rahimi E, Shakerian A, Jafariyan M, Ebrahimi M, & Riahi M. (2009). Occurrence of aflatoxin M1 in raw, pasteurized and UHT milk commercialized in Esfahan and Shahr-e Kord, Iran. Food Security. 1(3), 317-320.
  • 10. Ayyildiz, T. (2012). Organik Süt ve Süt Ürünlerinde Aflatoksin M1 Varlığının Araştırılması. (Yüksek Lisans Tezi Yüksek Lisans Tezi), Celal Bayar Üniversitesi, Manisa.
  • 11. Galvano F, Galofaro V, & Galvano G. (1996). Occurrence and stability of aflatoxin M1 in milk and milk products: a worldwide review. Journal of Food Protection. 59(10), 1079-1090.
  • 12. Kamka, A, Khaniki G.R. J, & Alavi S. (2011). Occurrence of aflatoxin M1 in raw milk produced in Ardebil of Iran. Iranian Journal of Environmental Health Science & Engineering, 8(2), 133-140.
  • 13. Tavakoli H.R, Kamkar A, Riazipour M, Mozaffari Nejad A, & Rafati H. (2013). Assessment of aflatoxin M1 levels by Enzyme-linked Immunosorbent Assay in yoghurt consumed in Tehran, Iran. Asian J Chem. 25, 2836-2838.
  • 14. Freeman B.A, Crapo J. (1982). Biology of disase, free rad cals and tissus injury Lab. Invest. 47(5), 412-425.
  • 15. Placer ZA, Cushman LL, Johnson BC. (1996). Estimation of product of lipid peroxidation (Malonyl dialdehyde) in biochemical systems, Anal Biochem. 16: 359-364. 16. Tirmenstein M.A, & Mangipudy R. (2014). Aflatoxin. In P. Wexler (Ed.), Encyclopedia of Toxicology, 104-106. Oxford: Academic Press.
  • 17. Agag B. (2004) Mycotoxins in foods and feeds: 1-aflatoxins. Ass. Univ. Bull. Environ Res. 7(1), 173- 205.
  • 18. Kamkar A, Fallah A. A, & Mozaffari Nejad A.S. (2014). The review of aflatoxin M1 contamination in milk and dairy products produced in Iran. Toxin Reviews, 33(4), 160-168.
  • 19. Tsakiris I.N, Tzatzarakis M.N, Alegakis A.K, et al. (2013). Risk assessment scenarios of children’s exposure to aflatoxin M1 residues in different milk types from the Greek market. Food and Chemical Toxicology, 56, 261-265.
  • 20. Bbosa G.S, Lubega A, Kyegombe D.B, et al. (2013). Review of the biological and health effects of aflatoxins on body organs and body systems. In M. Razzaghi-Abyaneh (Ed.), Aflatoxins - Recent Advances and Future Prospects. 239-266.
  • 21. Ulven S.M, Kirkhus B, Lamglait A, et al. (2011). Metabolic effects of krill oil are essentially similar to those of fish oil but at lower dose of EPA and DHA, in healthy volunteers. Lipids, 46(1), 37-46.
  • 22. Abbès S, Salah-Abbès JB, Abdel-Wahhab MA, & Ouslati R. (2010). Immunotoxicological and biochemical effects of Aflatoxins in rats prevented by Tunisian Montmorillonite with reference to HSCAS. Immunopharmacology and Immunotoxicology. 32(3), 514-522.
  • 23. Williams J. H, Phillips TD, Jolly PE et al. (2004). Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. The American Journal of Clinical Nutrition. 80(5), 1106- 1122.
  • 24. Diaz GJ, Sánchez MP (2015). Determination of aflatoxin M 1 in breast milk as a biomarker of maternal and infant exposure in Colombia. Food Addit Contam Part A. doi:10.1080/19440049. 1049563, 0049: 150511035939005.
  • 25. Atasever M, Yildirim Y, Atasever M, Tastekin A. (2014). Assessment of aflatoxin M1 in maternal breast milk in Eastern Turkey. Food Chem Toxicol. 66:147–149. doi: 10.1016/j.fct.2014.01.037.
  • 26. Mahdavi R, Nikniaz L, Arefhosseini SR, Vahed Jabbari M (2010). Determination of aflatoxin M1 in breast milk samples in Tabriz- Iran. Matern Child Health J. 14:141–145. doi:10.1007/s10995- 008-0439-9.
  • 27. Jurewicz J, Radwan M, Sobala W, Ligocka D, et al. (2014). Lifestyle and semen quality: role of modifiable risk factors. Syst Biol Reprod Med. 60(1):43-51. doi: 10.3109/19396368.2013.840687.
  • 28. Kunter İ, Hürer N &Gülcan H.O et al. (2016). Assessment of Aflatoxin M1 and Heavy Metal Levels in Mothers Breast Milk in Famagusta, Cyprus, Biol Trace Elem Res, DOI 10.1007/s12011-016-0750-z.
  • 29. Gürbay A, Sabuncuoğlu SA, Girgin G, Sahin G, et al. (2010). Exposure of newborns to aflatoxin M1 and B1 from mothers' breast milk in Ankara, Turkey. Food Chem Toxicol. 48(1):314-9. doi: 10.1016/j.fct.2009.10.016.
  • 30. Elzupir AO, Abas ARA, Fadul MH et al (2012). Aflatoxin M1 in breast milk of nursing Sudanese mothers. Mycotoxin Res. 28:131–134. doi:10.1007/s12550-012-0127-x
  • 31. El-Nezami, HS., Nicoletti, G., Neals, GE. Et al. (1995). Aflatoxin M1 in human breast milk samples from Victoria, Australia and Thailand, Fd. Chem. Toxic. 33(3): 173-179.
  • 32. Adejumo O, Atanda O, Raiola A et al. (2013). Correlation between aflatoxin M1 content of breast milk, dietary exposure to aflatoxin B1 and socioeconomic status of lactating mothers in Ogun State, Nigeria. Food Chem Toxicol. 56:171–177. doi: 10.1016/j.fct.2013.02.027.
  • 33. Shen HM, Shi CY, Lee HP, Ong CN. (1994). Aflatoxin B1-induced lipid peroxidation in rat liver. Toxicol Appl Pharmacol. 127(1):145-50.
  • 34. Souza MF, Tomé AR, Rao VS(1999). Effects of aluminosilicates on lipid peroxidation and antioxidants in aflatoxin B1-induced tissue injury in chickens. J Pharm Pharmacol.51(2):125-9.
  • 35. Grintzalis K, Vernardis SI, Klapa MI and Georgiou CD. (2014). Role of Oxidative Stress in Sclerotial Differentiation and Aflatoxin B1 Biosynthesis in Aspergillus flavus. Appl Environ Microbiol. 80(18): 5561–5571.
  • 36. Madali B, Ayaz A. (2017). Aflatoxin M1 in Dairy Products Aflatoxin M1 in Dairy Products: Exposure and Health Risks, H.Ü. Sağlık Bilimleri Fakültesi Dergisi 4(1):1-14.
Year 2020, Volume: 13 Issue: 2, 67 - 75, 31.12.2020

Abstract

Project Number

FEM15

References

  • 1. Jamali M., Ebrahimi M.A., Karimipour M, et al. (2012). An insight into the distribution, genetic diversity, and mycotoxin production of Aspergillus section Flavi in soils of pistachio orchards. Folia Microbiologica, 57(1), 27-36.
  • 2. Bennett JW. (2010). An overview of the genus Aspergillus, Caiser Academic Press, Portland, ISBN: 978-1-904455-53-0.
  • 3. Iqbal S.Z, Asi M.R, & Ariñ, A. (2013). Aflatoxins. In S. Maloy & K. Hughes (Eds.), Brenner's Encyclopedia of Genetics, USA: Institute of Molecular Scieces. (2 ed., pp. 43–47).
  • 4. Coppock RW, Christian R G, & Jacobsen BJ. (2012). Chapter 88- Aflatoxins. In R. C. Gupta (Ed.), Veterinary Toxicology (Second Edition), Boston: Academic Press. (pp. 1181-1199). 5. Kalantari H, & Kalantari G. (2007). Evaluation Of Aflatoxins Contamination In Baby Food Supplements (Mamana & Ghoncheh). Jundushapur Journal of Natural Pharmaceutical Products. 6(1), 42-50.
  • 6. Pitt, J I. (2014). Mycotoxins: Aflatoxins. In Y. Motarjemi (Ed.), Encyclopedia of Food Safety, 289-294) Waltham: Academic Press.
  • 7. Kamkar A. (2008). Detection of aflatoxin M1 in UHT milk sample by ELISA. J Vet Res. 63(2), 7-12.
  • 8. Mulunda M, Bakunzi F, Motsei L et al. (2013). A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review. In H. A. Makun (Ed.), Mycotoxin and Food Safety in Developing Countries. 39-60. Croatia: INTECH Open Access Publisher.
  • 9. Rahimi E, Shakerian A, Jafariyan M, Ebrahimi M, & Riahi M. (2009). Occurrence of aflatoxin M1 in raw, pasteurized and UHT milk commercialized in Esfahan and Shahr-e Kord, Iran. Food Security. 1(3), 317-320.
  • 10. Ayyildiz, T. (2012). Organik Süt ve Süt Ürünlerinde Aflatoksin M1 Varlığının Araştırılması. (Yüksek Lisans Tezi Yüksek Lisans Tezi), Celal Bayar Üniversitesi, Manisa.
  • 11. Galvano F, Galofaro V, & Galvano G. (1996). Occurrence and stability of aflatoxin M1 in milk and milk products: a worldwide review. Journal of Food Protection. 59(10), 1079-1090.
  • 12. Kamka, A, Khaniki G.R. J, & Alavi S. (2011). Occurrence of aflatoxin M1 in raw milk produced in Ardebil of Iran. Iranian Journal of Environmental Health Science & Engineering, 8(2), 133-140.
  • 13. Tavakoli H.R, Kamkar A, Riazipour M, Mozaffari Nejad A, & Rafati H. (2013). Assessment of aflatoxin M1 levels by Enzyme-linked Immunosorbent Assay in yoghurt consumed in Tehran, Iran. Asian J Chem. 25, 2836-2838.
  • 14. Freeman B.A, Crapo J. (1982). Biology of disase, free rad cals and tissus injury Lab. Invest. 47(5), 412-425.
  • 15. Placer ZA, Cushman LL, Johnson BC. (1996). Estimation of product of lipid peroxidation (Malonyl dialdehyde) in biochemical systems, Anal Biochem. 16: 359-364. 16. Tirmenstein M.A, & Mangipudy R. (2014). Aflatoxin. In P. Wexler (Ed.), Encyclopedia of Toxicology, 104-106. Oxford: Academic Press.
  • 17. Agag B. (2004) Mycotoxins in foods and feeds: 1-aflatoxins. Ass. Univ. Bull. Environ Res. 7(1), 173- 205.
  • 18. Kamkar A, Fallah A. A, & Mozaffari Nejad A.S. (2014). The review of aflatoxin M1 contamination in milk and dairy products produced in Iran. Toxin Reviews, 33(4), 160-168.
  • 19. Tsakiris I.N, Tzatzarakis M.N, Alegakis A.K, et al. (2013). Risk assessment scenarios of children’s exposure to aflatoxin M1 residues in different milk types from the Greek market. Food and Chemical Toxicology, 56, 261-265.
  • 20. Bbosa G.S, Lubega A, Kyegombe D.B, et al. (2013). Review of the biological and health effects of aflatoxins on body organs and body systems. In M. Razzaghi-Abyaneh (Ed.), Aflatoxins - Recent Advances and Future Prospects. 239-266.
  • 21. Ulven S.M, Kirkhus B, Lamglait A, et al. (2011). Metabolic effects of krill oil are essentially similar to those of fish oil but at lower dose of EPA and DHA, in healthy volunteers. Lipids, 46(1), 37-46.
  • 22. Abbès S, Salah-Abbès JB, Abdel-Wahhab MA, & Ouslati R. (2010). Immunotoxicological and biochemical effects of Aflatoxins in rats prevented by Tunisian Montmorillonite with reference to HSCAS. Immunopharmacology and Immunotoxicology. 32(3), 514-522.
  • 23. Williams J. H, Phillips TD, Jolly PE et al. (2004). Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. The American Journal of Clinical Nutrition. 80(5), 1106- 1122.
  • 24. Diaz GJ, Sánchez MP (2015). Determination of aflatoxin M 1 in breast milk as a biomarker of maternal and infant exposure in Colombia. Food Addit Contam Part A. doi:10.1080/19440049. 1049563, 0049: 150511035939005.
  • 25. Atasever M, Yildirim Y, Atasever M, Tastekin A. (2014). Assessment of aflatoxin M1 in maternal breast milk in Eastern Turkey. Food Chem Toxicol. 66:147–149. doi: 10.1016/j.fct.2014.01.037.
  • 26. Mahdavi R, Nikniaz L, Arefhosseini SR, Vahed Jabbari M (2010). Determination of aflatoxin M1 in breast milk samples in Tabriz- Iran. Matern Child Health J. 14:141–145. doi:10.1007/s10995- 008-0439-9.
  • 27. Jurewicz J, Radwan M, Sobala W, Ligocka D, et al. (2014). Lifestyle and semen quality: role of modifiable risk factors. Syst Biol Reprod Med. 60(1):43-51. doi: 10.3109/19396368.2013.840687.
  • 28. Kunter İ, Hürer N &Gülcan H.O et al. (2016). Assessment of Aflatoxin M1 and Heavy Metal Levels in Mothers Breast Milk in Famagusta, Cyprus, Biol Trace Elem Res, DOI 10.1007/s12011-016-0750-z.
  • 29. Gürbay A, Sabuncuoğlu SA, Girgin G, Sahin G, et al. (2010). Exposure of newborns to aflatoxin M1 and B1 from mothers' breast milk in Ankara, Turkey. Food Chem Toxicol. 48(1):314-9. doi: 10.1016/j.fct.2009.10.016.
  • 30. Elzupir AO, Abas ARA, Fadul MH et al (2012). Aflatoxin M1 in breast milk of nursing Sudanese mothers. Mycotoxin Res. 28:131–134. doi:10.1007/s12550-012-0127-x
  • 31. El-Nezami, HS., Nicoletti, G., Neals, GE. Et al. (1995). Aflatoxin M1 in human breast milk samples from Victoria, Australia and Thailand, Fd. Chem. Toxic. 33(3): 173-179.
  • 32. Adejumo O, Atanda O, Raiola A et al. (2013). Correlation between aflatoxin M1 content of breast milk, dietary exposure to aflatoxin B1 and socioeconomic status of lactating mothers in Ogun State, Nigeria. Food Chem Toxicol. 56:171–177. doi: 10.1016/j.fct.2013.02.027.
  • 33. Shen HM, Shi CY, Lee HP, Ong CN. (1994). Aflatoxin B1-induced lipid peroxidation in rat liver. Toxicol Appl Pharmacol. 127(1):145-50.
  • 34. Souza MF, Tomé AR, Rao VS(1999). Effects of aluminosilicates on lipid peroxidation and antioxidants in aflatoxin B1-induced tissue injury in chickens. J Pharm Pharmacol.51(2):125-9.
  • 35. Grintzalis K, Vernardis SI, Klapa MI and Georgiou CD. (2014). Role of Oxidative Stress in Sclerotial Differentiation and Aflatoxin B1 Biosynthesis in Aspergillus flavus. Appl Environ Microbiol. 80(18): 5561–5571.
  • 36. Madali B, Ayaz A. (2017). Aflatoxin M1 in Dairy Products Aflatoxin M1 in Dairy Products: Exposure and Health Risks, H.Ü. Sağlık Bilimleri Fakültesi Dergisi 4(1):1-14.
There are 34 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Aysel Güven

Barış Öztürk 0000-0002-0122-4757

H.ahmet Deveci

İnan Kaya

Project Number FEM15
Publication Date December 31, 2020
Submission Date June 21, 2020
Published in Issue Year 2020 Volume: 13 Issue: 2

Cite

APA Güven, A., Öztürk, B., Deveci, H., Kaya, İ. (2020). Investigation of the Relationship Between Blood Lipid Peroxidation and the Prevalence of Aflatoxin M1 in Milk Samples from Mothers and Cows Living in Kars and Surrounding Villages. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 13(2), 67-75.