PROTECTIVE EFFECTS OF SESAMOL AGAINST SECONDARY INJURY IN THE RAT MODEL OF TRAUMATIC BRAIN INJURY

Abstract

Objective: Sesamol is a powerful antioxidant, anti-inflammatory, antiapoptotic, and neuroprotective chemical. This study aimed to investigate the histopathological effects of sesamol in a rat traumatic brain injury (TBI) model. Material and Methods: Thirty-two male rats were divided into the following four groups: control, trauma, vehicle, and sesamol. All groups were subjected to TBI, and immediately after trauma saline and sesamol (100 mg/kg) were administered intraperitoneally to the vehicle and sesamol groups, respectively. At 24th hour of TBI, brain samples were collected, and histomorphological investigation of brain parenchyma was performed using electron and light microscopy. Results:On histopathological investigation, TBI induced brain injury was lesser than trauma and vehicle groups in the sesamol group. Compared to the trauma group, in the sesamol group there was less perivascular region edema. The neuronal processes of the sesamol group also displayed less intracellular edema and vacuoles. Conclusion:The results of the current study revealed for the first time that sesamol exhibits neuroprotective effects against TBI.

Keywords

• Neuroprotection
• sesamol
• traumatic brain injury

Travmatik Beyin Hasarı Sıçan Modelinde Sesamol’ün İkincil Yaralanmaya Karşı Koruyucu Etkileri

Abstract

Amaç: Sesamol güçlü bir antioksidan, antiinflammatuar, antiapoptotik ve nöroprotektif bir kimyasaldır. Bu çalışmada, sesamolün sıçan travmatik beyin hasarı (TBH) modelinde histopatolojik etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Otuz iki erkek sıçan dört gruba ayrıldı: kontrol, travma, sham ve sesamol. Travma, sham ve sesamol gruplarına ağırlık düşme yöntemi ile kapalı kafa travması uygulandı, travmadan hemen sonra sırasıyla sham ve sesamol gruplarına periton içine salin ve sesamol (100 mg/kg) uygulandı. Travmadan 24 saat sonra, beyin örnekleri alındı ve elektron ve ışık mikroskobu kullanılarak beyin korteksi histomorfolojik olarak incelendi. Bulgular: Histopatolojik değerlendirme sonucunda sesamol grubunda kafa travması ile indüklenen beyin korteksindeki hasar travma ve sham gruplarından daha azdı. Travma grubuna göre sesamol grubunda perivasküler alan ödemi daha azdı. Sesamol grubunun nöronal uzantılarında da daha az hücre içi ödem ve vakuoller izlendi. Sonuç: Bu çalışmanın sonuçları sesamolün TBH'na karşı nöroprotektif etkiler gösterdiğini ortaya koymuştur.

Keywords

• Nöroproteksiyon
• sesamol
• travmatik beyin hasarı

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