Are Serum Vitamin E Levels Associated with Severe Histological Features in Patients with Autoimmune Hepatitis?,

Abstract

Objective: Autoimmune hepatitis is an inflammation of the liver characterized by the presence of peri-portal hepatitis, hypergammaglobulinemia, and the serum autoantibodies. 

Vitamin E has anti-oxidant and anti-fibrotic properties and may potentially be effective for suppressing inflammation and liver fibrosis. Our aim is to determine vitamin E levels in patients, whose autoimmune liver diseases have been detected clinically and progression of fibrosis have been detected with liver biopsy samples, as well as toinvestigate the relationship between the vitamin E levels and fibrosis.

Material and Method: Sixty-three subjects aged between 19-75 were observed by prospective method with control. Serum samples of thirty-three patients with autoimmune liver disease, whose liver fibrosis levels were assessed by histopathology, and of 30 healthy controls were collected and Vitamin E levels were detected using a commercial vitamin E ELISA kit.

Results: This study was carried out with a total of 33 autoimmune hepatitis cases (27 female, 6 male) and 30 healthy controls (21 female and 9 male). The mean age of the patient and control group were 50.18±10.71 and 50.22 ± 10.03 years respectively. Mean vitamin E level was found to be 45.55±39.92 nmol/ml. Vitamin E levels of the patient group were significantly low compared to the control group (p<0.05). No statistically significant relationship was found between the fibrosis phase of the patient group and the vitamin E levels (p>0.05).

Conclusion: While vitamin E level of patients with autoimmune liver disease was significantly lower compared to the control group, there was no significant correlation between vitamin E level and fibrosis phases. As a conclusion, this study showed that vitamin E levels can not be used as a potential biomarker for the prediction of autoimmune liver diseases and it can not be involved in the treatment.

Keywords

• Vitmain E,autoimmune liver diseases,fibrosis

OTOİMMÜN HEPATİT TANILI HASTALARDA SERUM E VİTAMİNİ SEVİYELERİ HİSTOLOJİK AKTİVİTE ŞİDDETİ İLİŞKİLİ MİDİR?

Abstract

Amaç: Otoimmun hepatit karaciğerde histopatolojik olarak periportal hepatitle giden serumda hipergammaglobulinemi ve otoantikor pozitifliğinin eşlik ettiği karaciğer inflamasyonudur. Anti-oksidan ve anti-fibrotik özelliklere sahip olan vitamin E’nin inflamasyonu ve karaciğer fibrozisi baskılamada etkili olduğunu bidiren çalışmalar yayınlanmıştır. Bu çalışmanın amacı, otoimmün karaciğer hastalığı tespit edilmiş ve fibrozis oluşumu karaciğer biyopsi örnekleri ile belirlenmiş olgularda vitamin E düzeyini tespit etmek ve vitamin E düzeyleri ile fibrozis arasındaki ilişkiyi ortaya koymaktır.

Gereç ve Yöntem: Yaşları 19-75 arasında olan 63 vaka kontrollü prospektif metot ile incelendi. Karaciğer fibrozis dereceleri histopatoloji ile belirlenmiş olan 33 otoimmün hepatit hastası ve karaciğer hastalığı olmayan karaciğer biyopsisi yapılmamış 30 sağlıklı bireyin serumları toplandı ve bu serumlarda vitamin E düzeyleri ticari bir vitamin E ELISA kiti ile tespit edildi.

Bulgular: Bu çalışma, 27 kadın, 6 erkek toplam 33 otoimmun hepatit olgusu ve 21 kadın, 9 erkek toplam 30 sağlıklı kontrol grubu ile yapılmıştır. Hasta grubunun yaş ortalaması 50.18±10.71, sağlıklı kontrol grubunun yaş ortalaması 50.22±10.03 yıl olarak saptandı. Olguların vitamin E değerlerinmin ortalaması 45.55±39.92 nmol/ml olarak saptanmıştır. Hasta grubunun vitamin E değeri, kontrol grubuna göre istatistiksel olarak düşük saptanmıştır (p<0.05). Hasta grubunun fibrozis evresi ile vitamin E değerleri arasında istatistiksel olarak anlamlı ilişki saptanmamıştır (p>0.05).

Sonuç: Otoimmün karaciğer hastalarının vitamin E değeri, kontrol grubu olgulara göre, istatistiksel olarak anlamlı düzeyde düşük saptandığı halde, vitamin E seviyesiyle fibrozis evreleri arasında anlamlı bir korelasyonun olmadığı saptanmıştır. Dolayısıyla bu çalışma; vitamin E’nin, otoimmün karaciğer hastalıklarının değerlendirilmesinde potansiyel bir biyolojik belirteç olamayacağını ve tedavide yer alamayacağını ortaya koymuştur. Aynı zamanda vitamin E seviyesi ile bu hastalarda meydana gelen fibrozis evreleri arasında anlamlı bir ilişki yoktur.

Keywords

• Vitamin E,otoimmün hepatit,fibrozis

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