EFFECTS OF THYMOQUINONE, ZEOLITE AND PLATELET RICH PLASMA ON THE HEALING OF ISCHEMIC COLONIC ANASTOMOSIS

Abstract

Objective: Anastomosis on ischemic intestines is a problematic issue in daily surgical routine. In this study, we investigated the potential effects of thymoquinone, zeolite and platelet rich plasma.

Material and Methods: Fifty rats were divided into five groups.  In the sham group all rats underwent colonic anastomosis without any ischemic insult. Colonic anastomosis was performed after mesenteric ischemia was induced by clamping the superior mesenteric artery in other study groups.  In the control group, no medication was given to the rats after ishemic anastomosis. In Zeolite group and thymoquinone group, Zeolite and thymoquinone were applied locally on the anastomosis.  In the platelet rich plasma group, platelet rich plasma was applied locally on the anastomosis. All the rats were sacrified on the 10th day. Blood and tissue samples were retrieved.

Results: Hydroxyproline levels were not different between the groups. Bursting pressure was higher in the thymoquinone group than the control group. Reepithelization was higher than the zeolite group in the sham and thymoquinone groups. TNF-α and IL 1β levels were significantly higher in the sham group than all the other groups. TNF-α and IL 1β levels were also significantly higher in the thymoquinone, zeolite and platelet rich plasma groups than the control group and higher in the thymoquinone group than the zeolite and platelet rich plasma groups. Inflammation and ischemic necrosis levels were not different between the groups.

Conclusion: Our data is suggesting that thymoquinone and platelet rich plasma have positive effects on ischemic anastomosis healing, while thymoquinone has the best results.

Keywords

• Nigella sativa,zeolite,platelet rich plasma,ischemia,anastomosis

İskemik Kolon Anastomoz İyileşmesinde Timokinon, Zeolit ve Plateletten Zengin Plazmanın Etkinliği

Abstract

Amaç: Günlük cerrahi rutinde iskemik barsakların anastomozu önemli bir sorundur. Biz bu çalışmada timokinon, zeolit ve plateletten zengin plazmanın potansiyel etkilerini araştırdık.

Gereç ve Yöntemler: Toplam elli rat beş gruba ayrıldı. Sham grubuna iskemi olmaksızın kolonik anastomoz yapıldı. Çalışma gruplarında superior mezenterik arterin klemplenmesiyle oluşturulan mezenterik iskemi sonrası kolon anastomozu yapıldı. Kontrol grubuna iskemik anastomoz sonrası ilaç verilmedi. Zeolit, timokinon ve plateletten zengin plazma grubunda ilaç lokal olarak uygulandı. 10. günde ratlar sakrifiye edildi, doku ve kan örnekleri alındı.

Bulgular: Hidroksiprolin seviyelerinde gruplararası fark yoktu. Patlama basıncı timokinon grubunda kontrol grubuna göre daha yüksekti. Reepitelizasyon sham ve timokinon grubuna göre zeolit grubunda daha yüksekti. Sham grubunda diğer gruplara kıyasla TNF-α ve IL 1β düzeyleri anlamlı olarak yüksekti. Ayrıca TNF-α ve IL 1β düzeyleri timokinon, zeolit ve plateletten zengin plazma gruplarında kontrol grubuna göre anlamlı olarak yüksekti ve bu üçü arasında timokinon grubunda en yüksekti. İnflamasyon ve iskemik nekroz seviyeleri arasında gruplar arası anlamlı fark yoktu.

Sonuç: Sonuçlarımız timokinon ve plateletten zengin plazma’nin iskemik anastomoz iyileşmesi üzerine olumlu etkileri olduğunu göstermiş ve timokinon ile en iyi sonuçlar elde edilmiştir.

Keywords

• Nigella sativa,zeolit,plateletten zengin plazma,iskemi,anastomoz

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