The Comparison of VEGF Polymorphism Frequency between Cases with Low and High Ovarian Reserve

Year 2019, , 285 - 290, 31.12.2019

Derya Deveci

https://doi.org/10.24938/kutfd.537245

Abstract

Objective: Vascular endothelial growth factor (VEGF) is a cytokine
playing key role in ovarian angiogenesis. We aimed to investigate the possible relation
between VEGF gene polymorphism and ovarian reserve in infertile women suffering
from either low or high ovarian reserve.

Material and Methods: This study was conducted on 64 women suffering from polycystic
ovary syndrome (PCOS) and 30 women suffering from premature ovarian failure (POF).
VEGF-2578 C/A single nucleotide polymorphism (SNP) was investigated with polymerase
chain reaction (PCR) method on DNA extracted from peripheral venous blood sample.
Statistical analyses were done using SPSS 16.0 programme.

Results: There was no significant difference between PCOS and POF
groups for age, body mass index, TSH and prolactin levels. The genotype frequencies
of PCOS and POF groups were respectively 56.2% CC, 26.6% CA, 17.2% AA and 26.7%
CC, 53.3% CA, 20% AA. There was a significant difference between groups for genotype
frequencies (p=0.018). We observed minimal negative influence of mutant A allele
on ovarian reserve (95%CI= 0.12-0.22, OR: 0.5, p<0.01).

Conclusion: We observed a minimal negative influence of VEGF-2578 C/A
polymorphism on ovarian reserve. New and expanded population studies are needed
to discuss our results.

Keywords

VEGF, polymorphism, premature ovarian failure, polycystic ovary syndrome

DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI

Abstract

Amaç: Vasküler endotelyal büyüme faktörü
(VEGF), overyan anjiogenezde kilit rol oynayan bir sitokindir. Biz de çalışmamızda
düşük ve yüksek over rezervli infertil kadınlarda VEGF gen polimorfizminin over
rezervi ile olası ilişkisini araştırmayı amaçladık.

Gereç ve Yöntemler: Bu çalışmaya, polikistik over sendromlu
(PCOS) 64 kadın ile prematür overyan yetmezlikli (POF) 30 kadın dahil edildi. Periferik
venöz kan örneklerinden elde edilen DNA’da polimeraz zincir reaksiyonu (PCR) yöntemi
ile VEGF geni-2578 C/A tek nükleotid polimorfizmi (SNP) incelendi. İstatistiki analiz
SPSS 16.0 programı ile yapıldı.

Bulgular: PCOS ve POF gruplarının ortalama
yaş, vücut kitle indeksi, TSH ve prolaktin seviyeleri yönünden anlamlı bir fark
yoktu. PCOS grubu için genotip dağılımı %56.2 CC, %26.6 CA ve %17.2 AA iken, POF
grubu için ise %26.7 CC %53.3 CA ve %20 AA idi. Gruplar arasında genotip frekansları
bakımından farklılık saptandı (p = 0.018). Mutant A allelinin, over rezervini minimal
azaltıcı etkisi olduğu gözlendi (95%CI= 0.12-0.22, OR: 0.5, p<0.01).

Sonuç: Çalışmamızda gözlediğimiz VEGF
-2578 C/A polimorfizmindeki mutant A allelinin over rezervi üzerine olan etkisini
tartışmak için genişletilmiş popülasyon çalışmalarına ihtiyaç vardır.

Keywords

VEGF polimorfizmi, over rezervi, prematür overyan yetmezlik

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