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The Comparison of VEGF Polymorphism Frequency between Cases with Low and High Ovarian Reserve

Yıl 2019, , 285 - 290, 31.12.2019
https://doi.org/10.24938/kutfd.537245

Öz

Objective: Vascular endothelial growth factor (VEGF) is a cytokine
playing key role in ovarian angiogenesis. We aimed to investigate the possible relation
between VEGF gene polymorphism and ovarian reserve in infertile women suffering
from either low or high ovarian reserve.

Material and Methods: This study was conducted on 64 women suffering from polycystic
ovary syndrome (PCOS) and 30 women suffering from premature ovarian failure (POF).
VEGF-2578 C/A single nucleotide polymorphism (SNP) was investigated with polymerase
chain reaction (PCR) method on DNA extracted from peripheral venous blood sample.
Statistical analyses were done using SPSS 16.0 programme.

Results: There was no significant difference between PCOS and POF
groups for age, body mass index, TSH and prolactin levels. The genotype frequencies
of PCOS and POF groups were respectively 56.2% CC, 26.6% CA, 17.2% AA and 26.7%
CC, 53.3% CA, 20% AA. There was a significant difference between groups for genotype
frequencies (p=0.018). We observed minimal negative influence of mutant A allele
on ovarian reserve (95%CI= 0.12-0.22, OR: 0.5, p<0.01).

Conclusion: We observed a minimal negative influence of VEGF-2578 C/A
polymorphism on ovarian reserve. New and expanded population studies are needed
to discuss our results.

Kaynakça

  • 1. Lee EJ, Oh B, Lee JY, Kimm K, Park JM, Baek KH. Association study between single nucleotide polymorphisms in the VEGF gene and polycystic ovary syndrome. Fertil Steril. 2008;89(6):1751-9.
  • 1. Geva E, Jaffe RB. Role of vascular endothelial growth factor in ovarian physiology and pathology. Fertil Steril. 2000;74(3):429-38.
  • 2. Van Blerkom J, Antczak M, Schrader R. The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: association with vascular endothelial growth factor levels and perifollicular blood flow characteristics. Hum Reprod. 1997;12(5):1047-55.
  • 3. Sukur YE, Kivançli IB, Ozmen B. Ovarian aging and premature ovarian failure. J Turk Ger Gynecol Assoc. 2014;15(3):190-6.
  • 4. Fleming R, Seifer DB, Frattarelli JL, Ruman J. Assessing ovarian response: antral follicle count versus anti-Müllerian hormone. Reprod Biomed Online. 2015;31(4):486-96.
  • 5. Monniaux D, Clément F, Dalbiès-Tran R, Estienne A, Fabre S, Mansanet C, et al. The ovarian reserve of primordial follicles and the dynamic reserve of antral growing follicles: what is the link? Biol Reprod. 2014;90(4):85.
  • 6. Almawi WY, Gammoh E, Malalla ZH, Al-Madhi SA. Analysis of VEGFA variants and changes in VEGF levels underscores the contribution of VEGF to polycystic ovary syndrome. PLoS One. 2016;11(11):e0165636.
  • 7. Vural P, Kusku-Kiraz Z, Dogru-Abbasoglu S, Cil E, Karadag B, Akgül C et al. Vascular endothelial growth factor -2578 A/C, -460 T/C and +405 G/C polymorphisms in polycystic ovary syndrome. Eur J Obstet Gynecol Reprod Biol. 2009;147(1):57-60.
  • 8. Shelling AN. Premature ovarian failure. Reprod. 2010;140(5):633-41.
  • 9. Jeon YJ, Choi Y, Shim SH, Choi YS, Ko JJ, Yoon TK et al. Vascular endothelial growth factor gene polymorphisms in Korean patients with premature ovarian failure. Eur J Obstet Gynecol Reprod Biol. 2011;159(1):138-42.
  • 10. Qing Liu, Yan Li, Jian Zhao, Dong-Lan Sun, Ya-Nan Duan, Na Wang et al. Association of polymorphisms 21154G/A and 22578C/A in the vascular endothelial growth factor gene with decreased risk of endometriosis in Chinese women. Hum Reprod. 2009;24 (10):2660-6.
  • 11. Chen X, Li A, Chen W, Wei J, Fu J, Wang A. Differential gene expression in uterine endometrium during implantation in pigs. Biol Reprod. 2015;92(2):52.
  • 12. Sun Y, Chen M, Mao B, Cheng X, Zhang X, Xu C. Association between vascular endothelial growth factor polymorphism and recurrent pregnancy loss: A systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2017;211:169-76.
  • 13. Boudjenah R, Molina-Gomes D, Torre A, Boitrelle F, Taieb S, Dos Santos E et al. Associations between individual and combined polymorphisms of the TNF and VEGF genes and the embryo implantation rate in patients undergoing in vitro fertilization (IVF) programs. PLoS One. 2014;9(9):e108287.
  • 14. Boudjenah R, Molina-Gomes D, Wainer R, de Mazancourt P, Selva J, Vialard F. The vascular endothelial growth factor (VEGF) +405 G/C polymorphismand its relationship with recurrent implantation failure in women in an IVF programme with ICSI. J Assist Reprod Genet. 2012;29(12):1415-20.
  • 15. Yang M, Wang L, Wang X, Wang X, Yang Z, Li J. IL-6 Promotes FSH-induced VEGF expression through JAK/STAT3 signaling pathway in bovine granulosa cells. Cell Physiol Biochem. 2017;44(1):293-302.
  • 16. Kong HS, Lee J, Youm HW, Kim SK, Lee JR, Suh CS, Kim SH. Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice. PLoS One. 2017;12(9):e0184546.
  • 17. Torres-Ortiz MC, Gutiérrez-Ospina G, Gómez-Chavarín M, Murcia C, Alonso-Morales RA, Perera-Marín G. The presence of VEGF and Notch2 during preantral-antral follicular transition in infantile rats: Anatomical evidence and its implications. Gen Comp Endocrinol. 2017;249:82-92.
  • 18. Ben Salem A, Megdich F, Kacem O, Souayeh M, Hachani Ben Ali F, Hizem S et al. Vascular endothelial growth factor (VEGFA) gene variation in polycystic ovary syndrome in a Tunisian women population. BMC Genomics. 2016;17(9):748.
  • 19. Guruvaiah P, Govatati S, Reddy TV, Lomada D, Deenadayal M, Shivaji S et al. The VEGF +405 G>C 5' untranslated region polymorphism and risk of PCOS: a study in the South Indian Women. J Assist Reprod Genet. 2014;31(10):1383-9.
  • 20. Vural P, Kusku-Kiraz Z, Dogru-Abbasoglu S, Cil E, Karadag B, Uysal M. Vascular endothelial growth factor +405 G/C,-460 T/C and -2578 A/C polymorphisms are not associated with insulin resistance in polycystic ovary syndrome. Int J Immunogenet. 2010;37(4):239-43.
  • 21. Iaresko M, Kolesnikova E. The role of polymorphism 634 G/C (rs2010963) of VEGF-A gene in the development of hypertension and obesity in premenopausal women. Georgian Med News. 2016;256:33-7.
  • 22. Wei N, Chen Z, Xue Z, Zhu Y. Polymorphism of VEGF gene in susceptibility to chronic immune-mediated inflammatory diseases: a meta-analysis. Rheumatol Int. 2015;35(8):1351-60.
  • 23. Chen H, Zhang T, Gong B, Cao X. Association between VEGF -634G/C polymorphism and susceptibility to autoimmune diseases: a meta-analysis. Gene. 2015;558(2):181-6.
  • 24. Ebrahimi M, Akbari Asbagh F. The role of autoimmunity in premature ovarian failure. Iran J Reprod Med. 2015;13(8):461-72.
  • 25. Paradowska-Gorycka A, Pawlik A, Romanowska-Prochnicka K, Haladyj E, Malinowski D, Stypinska B et al. Relationship between VEGF gene polymorphisms and serum VEGF protein levels in patients with rheumatoid arthritis. PLoS One. 2016;11(8):e0160769.
  • 26. Del Bo R, Scarlato M, Ghezzi S, Maestroni A, Sjölind L, Forsblom C et al. VEGF gene variability and type 1 diabetes: evidence for a protective role. Immunogenetics. 2006;58(2-3):107-12.

DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI

Yıl 2019, , 285 - 290, 31.12.2019
https://doi.org/10.24938/kutfd.537245

Öz

Amaç: Vasküler endotelyal büyüme faktörü
(VEGF), overyan anjiogenezde kilit rol oynayan bir sitokindir. Biz de çalışmamızda
düşük ve yüksek over rezervli infertil kadınlarda VEGF gen polimorfizminin over
rezervi ile olası ilişkisini araştırmayı amaçladık.

Gereç ve Yöntemler: Bu çalışmaya, polikistik over sendromlu
(PCOS) 64 kadın ile prematür overyan yetmezlikli (POF) 30 kadın dahil edildi. Periferik
venöz kan örneklerinden elde edilen DNA’da polimeraz zincir reaksiyonu (PCR) yöntemi
ile VEGF geni-2578 C/A tek nükleotid polimorfizmi (SNP) incelendi. İstatistiki analiz
SPSS 16.0 programı ile yapıldı.

Bulgular: PCOS ve POF gruplarının ortalama
yaş, vücut kitle indeksi, TSH ve prolaktin seviyeleri yönünden anlamlı bir fark
yoktu. PCOS grubu için genotip dağılımı %56.2 CC, %26.6 CA ve %17.2 AA iken, POF
grubu için ise %26.7 CC %53.3 CA ve %20 AA idi. Gruplar arasında genotip frekansları
bakımından farklılık saptandı (p = 0.018). Mutant A allelinin, over rezervini minimal
azaltıcı etkisi olduğu gözlendi (95%CI= 0.12-0.22, OR: 0.5, p<0.01).

Sonuç: Çalışmamızda gözlediğimiz VEGF
-2578 C/A polimorfizmindeki mutant A allelinin over rezervi üzerine olan etkisini
tartışmak için genişletilmiş popülasyon çalışmalarına ihtiyaç vardır.

Kaynakça

  • 1. Lee EJ, Oh B, Lee JY, Kimm K, Park JM, Baek KH. Association study between single nucleotide polymorphisms in the VEGF gene and polycystic ovary syndrome. Fertil Steril. 2008;89(6):1751-9.
  • 1. Geva E, Jaffe RB. Role of vascular endothelial growth factor in ovarian physiology and pathology. Fertil Steril. 2000;74(3):429-38.
  • 2. Van Blerkom J, Antczak M, Schrader R. The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: association with vascular endothelial growth factor levels and perifollicular blood flow characteristics. Hum Reprod. 1997;12(5):1047-55.
  • 3. Sukur YE, Kivançli IB, Ozmen B. Ovarian aging and premature ovarian failure. J Turk Ger Gynecol Assoc. 2014;15(3):190-6.
  • 4. Fleming R, Seifer DB, Frattarelli JL, Ruman J. Assessing ovarian response: antral follicle count versus anti-Müllerian hormone. Reprod Biomed Online. 2015;31(4):486-96.
  • 5. Monniaux D, Clément F, Dalbiès-Tran R, Estienne A, Fabre S, Mansanet C, et al. The ovarian reserve of primordial follicles and the dynamic reserve of antral growing follicles: what is the link? Biol Reprod. 2014;90(4):85.
  • 6. Almawi WY, Gammoh E, Malalla ZH, Al-Madhi SA. Analysis of VEGFA variants and changes in VEGF levels underscores the contribution of VEGF to polycystic ovary syndrome. PLoS One. 2016;11(11):e0165636.
  • 7. Vural P, Kusku-Kiraz Z, Dogru-Abbasoglu S, Cil E, Karadag B, Akgül C et al. Vascular endothelial growth factor -2578 A/C, -460 T/C and +405 G/C polymorphisms in polycystic ovary syndrome. Eur J Obstet Gynecol Reprod Biol. 2009;147(1):57-60.
  • 8. Shelling AN. Premature ovarian failure. Reprod. 2010;140(5):633-41.
  • 9. Jeon YJ, Choi Y, Shim SH, Choi YS, Ko JJ, Yoon TK et al. Vascular endothelial growth factor gene polymorphisms in Korean patients with premature ovarian failure. Eur J Obstet Gynecol Reprod Biol. 2011;159(1):138-42.
  • 10. Qing Liu, Yan Li, Jian Zhao, Dong-Lan Sun, Ya-Nan Duan, Na Wang et al. Association of polymorphisms 21154G/A and 22578C/A in the vascular endothelial growth factor gene with decreased risk of endometriosis in Chinese women. Hum Reprod. 2009;24 (10):2660-6.
  • 11. Chen X, Li A, Chen W, Wei J, Fu J, Wang A. Differential gene expression in uterine endometrium during implantation in pigs. Biol Reprod. 2015;92(2):52.
  • 12. Sun Y, Chen M, Mao B, Cheng X, Zhang X, Xu C. Association between vascular endothelial growth factor polymorphism and recurrent pregnancy loss: A systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2017;211:169-76.
  • 13. Boudjenah R, Molina-Gomes D, Torre A, Boitrelle F, Taieb S, Dos Santos E et al. Associations between individual and combined polymorphisms of the TNF and VEGF genes and the embryo implantation rate in patients undergoing in vitro fertilization (IVF) programs. PLoS One. 2014;9(9):e108287.
  • 14. Boudjenah R, Molina-Gomes D, Wainer R, de Mazancourt P, Selva J, Vialard F. The vascular endothelial growth factor (VEGF) +405 G/C polymorphismand its relationship with recurrent implantation failure in women in an IVF programme with ICSI. J Assist Reprod Genet. 2012;29(12):1415-20.
  • 15. Yang M, Wang L, Wang X, Wang X, Yang Z, Li J. IL-6 Promotes FSH-induced VEGF expression through JAK/STAT3 signaling pathway in bovine granulosa cells. Cell Physiol Biochem. 2017;44(1):293-302.
  • 16. Kong HS, Lee J, Youm HW, Kim SK, Lee JR, Suh CS, Kim SH. Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice. PLoS One. 2017;12(9):e0184546.
  • 17. Torres-Ortiz MC, Gutiérrez-Ospina G, Gómez-Chavarín M, Murcia C, Alonso-Morales RA, Perera-Marín G. The presence of VEGF and Notch2 during preantral-antral follicular transition in infantile rats: Anatomical evidence and its implications. Gen Comp Endocrinol. 2017;249:82-92.
  • 18. Ben Salem A, Megdich F, Kacem O, Souayeh M, Hachani Ben Ali F, Hizem S et al. Vascular endothelial growth factor (VEGFA) gene variation in polycystic ovary syndrome in a Tunisian women population. BMC Genomics. 2016;17(9):748.
  • 19. Guruvaiah P, Govatati S, Reddy TV, Lomada D, Deenadayal M, Shivaji S et al. The VEGF +405 G>C 5' untranslated region polymorphism and risk of PCOS: a study in the South Indian Women. J Assist Reprod Genet. 2014;31(10):1383-9.
  • 20. Vural P, Kusku-Kiraz Z, Dogru-Abbasoglu S, Cil E, Karadag B, Uysal M. Vascular endothelial growth factor +405 G/C,-460 T/C and -2578 A/C polymorphisms are not associated with insulin resistance in polycystic ovary syndrome. Int J Immunogenet. 2010;37(4):239-43.
  • 21. Iaresko M, Kolesnikova E. The role of polymorphism 634 G/C (rs2010963) of VEGF-A gene in the development of hypertension and obesity in premenopausal women. Georgian Med News. 2016;256:33-7.
  • 22. Wei N, Chen Z, Xue Z, Zhu Y. Polymorphism of VEGF gene in susceptibility to chronic immune-mediated inflammatory diseases: a meta-analysis. Rheumatol Int. 2015;35(8):1351-60.
  • 23. Chen H, Zhang T, Gong B, Cao X. Association between VEGF -634G/C polymorphism and susceptibility to autoimmune diseases: a meta-analysis. Gene. 2015;558(2):181-6.
  • 24. Ebrahimi M, Akbari Asbagh F. The role of autoimmunity in premature ovarian failure. Iran J Reprod Med. 2015;13(8):461-72.
  • 25. Paradowska-Gorycka A, Pawlik A, Romanowska-Prochnicka K, Haladyj E, Malinowski D, Stypinska B et al. Relationship between VEGF gene polymorphisms and serum VEGF protein levels in patients with rheumatoid arthritis. PLoS One. 2016;11(8):e0160769.
  • 26. Del Bo R, Scarlato M, Ghezzi S, Maestroni A, Sjölind L, Forsblom C et al. VEGF gene variability and type 1 diabetes: evidence for a protective role. Immunogenetics. 2006;58(2-3):107-12.
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm MAK
Yazarlar

Derya Deveci Bu kişi benim 0000-0002-9867-0867

Yayımlanma Tarihi 31 Aralık 2019
Gönderilme Tarihi 8 Mart 2019
Yayımlandığı Sayı Yıl 2019

Kaynak Göster

APA Deveci, D. (2019). DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI. The Journal of Kırıkkale University Faculty of Medicine, 21(3), 285-290. https://doi.org/10.24938/kutfd.537245
AMA Deveci D. DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI. Kırıkkale Üni Tıp Derg. Aralık 2019;21(3):285-290. doi:10.24938/kutfd.537245
Chicago Deveci, Derya. “DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI”. The Journal of Kırıkkale University Faculty of Medicine 21, sy. 3 (Aralık 2019): 285-90. https://doi.org/10.24938/kutfd.537245.
EndNote Deveci D (01 Aralık 2019) DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI. The Journal of Kırıkkale University Faculty of Medicine 21 3 285–290.
IEEE D. Deveci, “DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI”, Kırıkkale Üni Tıp Derg, c. 21, sy. 3, ss. 285–290, 2019, doi: 10.24938/kutfd.537245.
ISNAD Deveci, Derya. “DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI”. The Journal of Kırıkkale University Faculty of Medicine 21/3 (Aralık 2019), 285-290. https://doi.org/10.24938/kutfd.537245.
JAMA Deveci D. DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI. Kırıkkale Üni Tıp Derg. 2019;21:285–290.
MLA Deveci, Derya. “DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI”. The Journal of Kırıkkale University Faculty of Medicine, c. 21, sy. 3, 2019, ss. 285-90, doi:10.24938/kutfd.537245.
Vancouver Deveci D. DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI. Kırıkkale Üni Tıp Derg. 2019;21(3):285-90.

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