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Kortikosteroid ve Antihistaminik Enjeksiyonların Sıçan Mesane Dokusunda Soğuk Kaynaklı Stres Üzerine Etkileri: Deneysel Bir Çalışma

Year 2020, Volume: 22 Issue: 1, 11 - 19, 30.04.2020
https://doi.org/10.24938/kutfd.621514

Abstract

Amaç: Soğuk, mesane fonksiyonunda değişik şekillerde hasara neden olabilen stres indükleyici bir faktördür. Bu çalışmada kortikosteroid ve antihistaminik tedavisinin akut ve kronik olarak indüklenmiş soğuk stres üzerine etkilerini araştırmak amaçlanmıştır.
Gereç ve Yöntemler: 42 adet dişi sıçanrastgele yedi gruba ayrılmıştır. Gruplar şu şekildedir;
Kontrol grubu, Akut soğuk stress (ACS) grubu; ACS+ kortikosteroid (CORT) grubu; ACS+CORT+antihistaminik grubu; Kronik soğuk stress (CCS) grubu; CSS CORT grubu; CSS+CORT+antihistaminik grubu. Bu uygulamalardan sonraki 15. günde, genel anestezi altında sıçanlara sistektomi uygulandı ve mesaneleri çıkarıldı. Çıkarılan dokular %10 nötral tamponlu formalin içerisinde fikse edilip takip edildikten sonra hematoksilen eozin ve Toluidin mavisi ile boyandı.
Bulgular: Mast hücre sayısına göre yapılan değerlendirmede, kontrol grubuna kıyasla en yüksek değer CCS grubunda gözlenmiştir. En düşük değer CCS + CORT grubunda elde edilmiştir. CCS grupları arasındaki karşılaştırmaya göre, lamina propriadaki en yüksek polimorfonükleer lökosit (PNL) değerleri CCS grubunda görülürken, CORT ve CORT+Antihistaminik tedavilerinin, lamina propriadaki PNL değerlerini önemli ölçüde azalttığı gözlendi. Epiteldeki PNL sayıları ACS ve CCS gruplarında yüksek iken, CORT ve/veya antihistaminik ile tedavi edilen ACS ve CCS gruplarındaki sonuçların kontrol grubundakilerle benzer olduğu ortaya çıkmıştır. CORT’a ek olarak antihistaminik enjeksiyonun, CCS'deki epitelde bulunan lenfosit sayısını, sadece CORT'dan daha verimli bir şekilde azalttığı görüldü.
Sonuç: Mevcut çalışma, kortikosteroid tedavisinin, enflamatuar hücre infiltrasyonunu ve mast hücre sayısını azalttığını ortaya koymuştur. Özellikle kronik soğuk stresde daha belirgin bir iyileşme olduğu gözlenmektedir.

References

  • 1. Kaushik S, Kaur J. Chronic cold exposure affects the antioxidant defense system in various rat tissues. Clin Chim Acta. 2003;333(1):69-77.
  • 2. Zhao FQ, Zhang ZW, Yao HD, Wang LL, Liu T, Yu XY et al. Effects of cold stress on mRNA expression of immunoglobulin and cytokine in the small intestine of broiles. Res Vet Sci. 2013;95(1):146-55.
  • 3. Xu B, Lang L, Li SZ, Guo JR, Wang JF, Wang D et al. Cortisol Excess-mediated mitochondrial damage induced hippocampal neuronal apoptosis in mice following cold exposure. Cells. 2019;8(6):1-23.
  • 4. Giesbrecht GC. Cold stress, near drowning and accidental hypothermia: a review. Aviat Space Environ Med. 2000;71(7):733-52.
  • 5. Yıldızel SA, Yıldırım MS, Sarıcıoğlu P. Bir şantiyede soğuk hava koşullarının işçi sağlığı ve performansı üzerine etkisinin incelenmesi. Erdem Ş, Emekli A, Baradan S, İncir A, Yavuz A. 5. İşçi Sağlığı ve İş Güvenliği Sempozyumu. 5. İşçi Sağlığı ve İş Güvenliği Sempozyumu Bildiri Kitabı; 2018, İstanbul, Türkiye. İzmir: Altındağ Grafik Matbaacılık; 2015. 111-8.
  • 6. Nagai H, Morise K, Mitsuma T, Furusawa A, Kaneko H, Uchida K et al. Effect of cold restraint stress on immunoreactive thyrotropin-releasing hormone and immunoreactive somatostatin in the rat stomach. J Gastroenterol. 1995;30(2):142-8.
  • 7. Uguralp S, Ozturk F, Aktay G, Cetin A, Gursoy S. The antioxidant effects of dry apricot in the various tissues of rats with induced cold restraint stress. Nat Prod Res. 2012;26(16):15351538.
  • 8. Kanay Z, Sert C, Işık B, Kurt D, Güzel C, Mete N. Stres uygulanan ratlarda eksojen glutatyon uygulamasının mide mukozal bariyeri üzerindeki etkileri. Turk J Gastroenterol. 1998;9(3):226-8.
  • 9. Coskun T, Yeğen BC, Alican I, Peker O, Kurtel H. Cold restraint stress-induced gastric mucosal dysfunction. Role of nitric oxide. Dig Dis Sci. 1996;41(5):956-63.
  • 10. Gue M, Fioramonti J, Bueno L. Comparative influences of acoustic and cold stress on gastrointestinal transit in mice. Am J Physiol. 1987;253(2):G124-G128.
  • 11. Debnath S, Guha D. Role of Moringa oleifera on enterochromaffin cell count and serotonin content of experimental ulcer model. Indian J Exp Biol. 2007;45(8):726-31.
  • 12. Singh P, Dutta SR, Guha D. Gastric mucosal protection by aegle marmelos against gastric mucosal damage: Role of enterochromaffin cell and serotonin. Saudi J Gastroenterol. 2015;21(1):35-42.
  • 13. Zhang ZW, Lv ZH, Li JL, Li S, Xu SW, Wang XL. Effects of cold stress on nitric oxide in duodenum of chicks. Poult Sci. 2011;90(7):1555-61.
  • 14. Kaushik S, Kaur J. Effect of chronic cold stress on intestinal epithelial cell proliferation and inflammation in rats. Stress. 2005;8(3):191-7.
  • 15. Uguralp S, Mizrak B, Karabulut AB, Gurbuz N, Demircan M. Interferon alpha reduces the development of experimental necrotizing enterocolitis. Cytokine. 2004;25(5):204-11.
  • 16. Chen K, Sun D, Qu S, Chen Y, Wang J, Zhou L et al. Prenatal cold exposure causes hypertension in offspring by hyperactivity of the sympathetic nervous system. Clin Sci. 2019;133(9):1097-13.
  • 17. Ercan F, San T, Cavdar S. The effects of cold-restraint stress on urinary bladder wall compared with interstitial cystitis morphology. Urol Res. 1999;27(6):454-61.
  • 18. Demir A, Onol FF, Ercan F, Tarcan T. Effect of cold-induced stress on rat bladder tissue contractility and histomorphology. Neurourol Urodyn. 2007;26(2):296-301.
  • 19. Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Accessed date: 04 November 2017: https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf.
  • 20. Skutechi R, Jalali R, Draganska E, Cymes I, Romazsko J, Glinska-Lewczuk K. UTCI as a bio-meteorological tool in the assessment of cold induced stress as a risk factor for hypertension. Sci Total Environ. 2019;688:970-5.
  • 21. Kanayama N, Tsujimura R, She L, Maehara K, Terao T. Cold-induced stress stimulates the sympathetic nervous system, causing hypertension and proteinuria in rats. J Hypertens. 1997;15(4):383-9.
  • 22. Chen K, Li X, Song G, Zhou T, Long Y, Li Q et al. Deficiency in the membrane protein Tmbim3a/Grinaa initiates cold-induced ER stress and cell death by activating an intrinsic apoptotic pathway in zebrafish. J Biol Chem. 2019;294(30):11445-57.
  • 23. Lu DL, Ma Q, Sun SX, Zhang H, Chen LQ, Zhang ML et al. Reduced oxidative stress increases acute cold stress tolerance in zebrafish. Comp Biochem Physiol A Mol Integr Physiol. 2019;235:166-73.
  • 24. Geirsson G, Lindstrom S, Fall M. The bladder cooling reflex and the use of cooling as stimulus to the lower urinary tract. J Urol. 1999;162(6):1890-96.
  • 25. Uvin P, Franken J, Pinto S, Rietjens R, Grammet L, Deruyver Y et al. Essential Role of Transient Receptor Potential M8 (TRPM8) in a Model of Acute Cold-induced Urinary Urgency. Eur Urol. 2015;68(4):655-61.
  • 26. Ankara Üniversitesi Tıp Fakültesi İç Hastalıkları Anabilim Dalı Romatoloji Bilim Dalı. Erişim tarihi: 05.09.2019: http://ichastaliklariromatoloji.medicine.ankara.edu.tr/wp-content/uploads/site s/680/2014/02/Kortikosteroidler.

EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY

Year 2020, Volume: 22 Issue: 1, 11 - 19, 30.04.2020
https://doi.org/10.24938/kutfd.621514

Abstract

Objective: Cold is a stress-inducing factor that can cause changes in bladder function in various ways. The present study is intended to investigate the effects of corticosteroid and antihistaminic treatment on acutely and chronically induced cold stress.
Material and Methods: Forty-two female rats were randomly divided into seven groups as follows: Control group; Acute cold-stress (ACS) group; ACS+ corticosteroid (CORT) group; ACS+CORT + Antihistaminic group; Cronic cold-stres (CCS) group; CSS+CORT group; CSS+CORT+Antihistaminic group. On the 15th day after these treatments, bladders of the rats were harvested for histopathological examinations under general anesthesia and fixed with 10% neutral buffered formaline. Hematoxylin and eosin and toluidine blue stainings were performed.
Results: The comparison based on mast cell count yielded the highest value in the CCS group in comparison to the control group. The lowest value was harvested in the CCS + CORT group. The comparison between the CCS groups revealed the highest polymorphonuclear leucocyte (PNL) values in the lamina propria in the CCS group, whereas the CORT and CORT + Antihistaminic treatments were found to have significantly decreased the PNL values in the lamina propria. While the PNL counts in the epithelium were high in the ACS and CCS groups, the results in the ACS and CCS groups that were treated with CORT and/or antihistaminic were revealed to be similar with those in the control group. It was discovered that antihistaminic injection in addition to CORT decreased the lymphocyte counts in epithelium in CCS more efficiently than CORT alone did.
Conclusion: The present research revealed that corticosteroid treatment reduced inflammatory cell infiltration and decreased mast cell count. A more evident amelioration was observed particularly in chronic cold stress.

References

  • 1. Kaushik S, Kaur J. Chronic cold exposure affects the antioxidant defense system in various rat tissues. Clin Chim Acta. 2003;333(1):69-77.
  • 2. Zhao FQ, Zhang ZW, Yao HD, Wang LL, Liu T, Yu XY et al. Effects of cold stress on mRNA expression of immunoglobulin and cytokine in the small intestine of broiles. Res Vet Sci. 2013;95(1):146-55.
  • 3. Xu B, Lang L, Li SZ, Guo JR, Wang JF, Wang D et al. Cortisol Excess-mediated mitochondrial damage induced hippocampal neuronal apoptosis in mice following cold exposure. Cells. 2019;8(6):1-23.
  • 4. Giesbrecht GC. Cold stress, near drowning and accidental hypothermia: a review. Aviat Space Environ Med. 2000;71(7):733-52.
  • 5. Yıldızel SA, Yıldırım MS, Sarıcıoğlu P. Bir şantiyede soğuk hava koşullarının işçi sağlığı ve performansı üzerine etkisinin incelenmesi. Erdem Ş, Emekli A, Baradan S, İncir A, Yavuz A. 5. İşçi Sağlığı ve İş Güvenliği Sempozyumu. 5. İşçi Sağlığı ve İş Güvenliği Sempozyumu Bildiri Kitabı; 2018, İstanbul, Türkiye. İzmir: Altındağ Grafik Matbaacılık; 2015. 111-8.
  • 6. Nagai H, Morise K, Mitsuma T, Furusawa A, Kaneko H, Uchida K et al. Effect of cold restraint stress on immunoreactive thyrotropin-releasing hormone and immunoreactive somatostatin in the rat stomach. J Gastroenterol. 1995;30(2):142-8.
  • 7. Uguralp S, Ozturk F, Aktay G, Cetin A, Gursoy S. The antioxidant effects of dry apricot in the various tissues of rats with induced cold restraint stress. Nat Prod Res. 2012;26(16):15351538.
  • 8. Kanay Z, Sert C, Işık B, Kurt D, Güzel C, Mete N. Stres uygulanan ratlarda eksojen glutatyon uygulamasının mide mukozal bariyeri üzerindeki etkileri. Turk J Gastroenterol. 1998;9(3):226-8.
  • 9. Coskun T, Yeğen BC, Alican I, Peker O, Kurtel H. Cold restraint stress-induced gastric mucosal dysfunction. Role of nitric oxide. Dig Dis Sci. 1996;41(5):956-63.
  • 10. Gue M, Fioramonti J, Bueno L. Comparative influences of acoustic and cold stress on gastrointestinal transit in mice. Am J Physiol. 1987;253(2):G124-G128.
  • 11. Debnath S, Guha D. Role of Moringa oleifera on enterochromaffin cell count and serotonin content of experimental ulcer model. Indian J Exp Biol. 2007;45(8):726-31.
  • 12. Singh P, Dutta SR, Guha D. Gastric mucosal protection by aegle marmelos against gastric mucosal damage: Role of enterochromaffin cell and serotonin. Saudi J Gastroenterol. 2015;21(1):35-42.
  • 13. Zhang ZW, Lv ZH, Li JL, Li S, Xu SW, Wang XL. Effects of cold stress on nitric oxide in duodenum of chicks. Poult Sci. 2011;90(7):1555-61.
  • 14. Kaushik S, Kaur J. Effect of chronic cold stress on intestinal epithelial cell proliferation and inflammation in rats. Stress. 2005;8(3):191-7.
  • 15. Uguralp S, Mizrak B, Karabulut AB, Gurbuz N, Demircan M. Interferon alpha reduces the development of experimental necrotizing enterocolitis. Cytokine. 2004;25(5):204-11.
  • 16. Chen K, Sun D, Qu S, Chen Y, Wang J, Zhou L et al. Prenatal cold exposure causes hypertension in offspring by hyperactivity of the sympathetic nervous system. Clin Sci. 2019;133(9):1097-13.
  • 17. Ercan F, San T, Cavdar S. The effects of cold-restraint stress on urinary bladder wall compared with interstitial cystitis morphology. Urol Res. 1999;27(6):454-61.
  • 18. Demir A, Onol FF, Ercan F, Tarcan T. Effect of cold-induced stress on rat bladder tissue contractility and histomorphology. Neurourol Urodyn. 2007;26(2):296-301.
  • 19. Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Accessed date: 04 November 2017: https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf.
  • 20. Skutechi R, Jalali R, Draganska E, Cymes I, Romazsko J, Glinska-Lewczuk K. UTCI as a bio-meteorological tool in the assessment of cold induced stress as a risk factor for hypertension. Sci Total Environ. 2019;688:970-5.
  • 21. Kanayama N, Tsujimura R, She L, Maehara K, Terao T. Cold-induced stress stimulates the sympathetic nervous system, causing hypertension and proteinuria in rats. J Hypertens. 1997;15(4):383-9.
  • 22. Chen K, Li X, Song G, Zhou T, Long Y, Li Q et al. Deficiency in the membrane protein Tmbim3a/Grinaa initiates cold-induced ER stress and cell death by activating an intrinsic apoptotic pathway in zebrafish. J Biol Chem. 2019;294(30):11445-57.
  • 23. Lu DL, Ma Q, Sun SX, Zhang H, Chen LQ, Zhang ML et al. Reduced oxidative stress increases acute cold stress tolerance in zebrafish. Comp Biochem Physiol A Mol Integr Physiol. 2019;235:166-73.
  • 24. Geirsson G, Lindstrom S, Fall M. The bladder cooling reflex and the use of cooling as stimulus to the lower urinary tract. J Urol. 1999;162(6):1890-96.
  • 25. Uvin P, Franken J, Pinto S, Rietjens R, Grammet L, Deruyver Y et al. Essential Role of Transient Receptor Potential M8 (TRPM8) in a Model of Acute Cold-induced Urinary Urgency. Eur Urol. 2015;68(4):655-61.
  • 26. Ankara Üniversitesi Tıp Fakültesi İç Hastalıkları Anabilim Dalı Romatoloji Bilim Dalı. Erişim tarihi: 05.09.2019: http://ichastaliklariromatoloji.medicine.ankara.edu.tr/wp-content/uploads/site s/680/2014/02/Kortikosteroidler.
There are 26 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Hüseyin Avni Eroğlu 0000-0002-1040-3255

Başak Büyük 0000-0003-1817-2241

Yasemen Adalı 0000-0002-8004-7364

Aslan Demir This is me 0000-0002-3089-7870

Publication Date April 30, 2020
Submission Date September 18, 2019
Published in Issue Year 2020 Volume: 22 Issue: 1

Cite

APA Eroğlu, H. A., Büyük, B., Adalı, Y., Demir, A. (2020). EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 22(1), 11-19. https://doi.org/10.24938/kutfd.621514
AMA Eroğlu HA, Büyük B, Adalı Y, Demir A. EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY. Kırıkkale Uni Med J. April 2020;22(1):11-19. doi:10.24938/kutfd.621514
Chicago Eroğlu, Hüseyin Avni, Başak Büyük, Yasemen Adalı, and Aslan Demir. “EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY”. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 22, no. 1 (April 2020): 11-19. https://doi.org/10.24938/kutfd.621514.
EndNote Eroğlu HA, Büyük B, Adalı Y, Demir A (April 1, 2020) EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 22 1 11–19.
IEEE H. A. Eroğlu, B. Büyük, Y. Adalı, and A. Demir, “EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY”, Kırıkkale Uni Med J, vol. 22, no. 1, pp. 11–19, 2020, doi: 10.24938/kutfd.621514.
ISNAD Eroğlu, Hüseyin Avni et al. “EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY”. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 22/1 (April 2020), 11-19. https://doi.org/10.24938/kutfd.621514.
JAMA Eroğlu HA, Büyük B, Adalı Y, Demir A. EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY. Kırıkkale Uni Med J. 2020;22:11–19.
MLA Eroğlu, Hüseyin Avni et al. “EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY”. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, vol. 22, no. 1, 2020, pp. 11-19, doi:10.24938/kutfd.621514.
Vancouver Eroğlu HA, Büyük B, Adalı Y, Demir A. EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY. Kırıkkale Uni Med J. 2020;22(1):11-9.

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