Kortikosteroid ve Antihistaminik Enjeksiyonların Sıçan Mesane Dokusunda Soğuk Kaynaklı Stres Üzerine Etkileri: Deneysel Bir Çalışma

Year 2020, Volume: 22 Issue: 1, 11 - 19, 30.04.2020

Hüseyin Avni Eroğlu , Başak Büyük , Yasemen Adalı , Aslan Demir

https://doi.org/10.24938/kutfd.621514

Abstract

Amaç: Soğuk, mesane fonksiyonunda değişik şekillerde hasara neden olabilen stres indükleyici bir faktördür. Bu çalışmada kortikosteroid ve antihistaminik tedavisinin akut ve kronik olarak indüklenmiş soğuk stres üzerine etkilerini araştırmak amaçlanmıştır.

Gereç ve Yöntemler: 42 adet dişi sıçanrastgele yedi gruba ayrılmıştır. Gruplar şu şekildedir;

Kontrol grubu, Akut soğuk stress (ACS) grubu; ACS+ kortikosteroid (CORT) grubu; ACS+CORT+antihistaminik grubu; Kronik soğuk stress (CCS) grubu; CSS CORT grubu; CSS+CORT+antihistaminik grubu. Bu uygulamalardan sonraki 15. günde, genel anestezi altında sıçanlara sistektomi uygulandı ve mesaneleri çıkarıldı. Çıkarılan dokular %10 nötral tamponlu formalin içerisinde fikse edilip takip edildikten sonra hematoksilen eozin ve Toluidin mavisi ile boyandı.

Bulgular: Mast hücre sayısına göre yapılan değerlendirmede, kontrol grubuna kıyasla en yüksek değer CCS grubunda gözlenmiştir. En düşük değer CCS + CORT grubunda elde edilmiştir. CCS grupları arasındaki karşılaştırmaya göre, lamina propriadaki en yüksek polimorfonükleer lökosit (PNL) değerleri CCS grubunda görülürken, CORT ve CORT+Antihistaminik tedavilerinin, lamina propriadaki PNL değerlerini önemli ölçüde azalttığı gözlendi. Epiteldeki PNL sayıları ACS ve CCS gruplarında yüksek iken, CORT ve/veya antihistaminik ile tedavi edilen ACS ve CCS gruplarındaki sonuçların kontrol grubundakilerle benzer olduğu ortaya çıkmıştır. CORT’a ek olarak antihistaminik enjeksiyonun, CCS'deki epitelde bulunan lenfosit sayısını, sadece CORT'dan daha verimli bir şekilde azalttığı görüldü.

Sonuç: Mevcut çalışma, kortikosteroid tedavisinin, enflamatuar hücre infiltrasyonunu ve mast hücre sayısını azalttığını ortaya koymuştur. Özellikle kronik soğuk stresde daha belirgin bir iyileşme olduğu gözlenmektedir.

Keywords

Soğuk stress, sistit, sıçan, kortikosteroid, antihistaminik

EFFECTS OF CORTICOSTEROID AND ANTIHISTAMINIC INJECTIONS ON COLD-INDUCED STRESS ON RAT BLADDER TISSUE: AN EXPERIMENTAL STUDY

Abstract

Objective: Cold is a stress-inducing factor that can cause changes in bladder function in various ways. The present study is intended to investigate the effects of corticosteroid and antihistaminic treatment on acutely and chronically induced cold stress.

Material and Methods: Forty-two female rats were randomly divided into seven groups as follows: Control group; Acute cold-stress (ACS) group; ACS+ corticosteroid (CORT) group; ACS+CORT + Antihistaminic group; Cronic cold-stres (CCS) group; CSS+CORT group; CSS+CORT+Antihistaminic group. On the 15th day after these treatments, bladders of the rats were harvested for histopathological examinations under general anesthesia and fixed with 10% neutral buffered formaline. Hematoxylin and eosin and toluidine blue stainings were performed.

Results: The comparison based on mast cell count yielded the highest value in the CCS group in comparison to the control group. The lowest value was harvested in the CCS + CORT group. The comparison between the CCS groups revealed the highest polymorphonuclear leucocyte (PNL) values in the lamina propria in the CCS group, whereas the CORT and CORT + Antihistaminic treatments were found to have significantly decreased the PNL values in the lamina propria. While the PNL counts in the epithelium were high in the ACS and CCS groups, the results in the ACS and CCS groups that were treated with CORT and/or antihistaminic were revealed to be similar with those in the control group. It was discovered that antihistaminic injection in addition to CORT decreased the lymphocyte counts in epithelium in CCS more efficiently than CORT alone did.

Conclusion: The present research revealed that corticosteroid treatment reduced inflammatory cell infiltration and decreased mast cell count. A more evident amelioration was observed particularly in chronic cold stress.

Keywords

Cold stress, cystitis, rat, corticosteroid, antihistaminic

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