Gıda Kısıtlaması Yapılan Ratlarda Karaciğerde Bazı Lipojenik Genlerin İfade Düzeyleri Ve Yağ Asidi Profili

Öz

Bu çalışma gıda kısıtlamasının karaciğerdeki yağ asidi profili ve majör lipojenik genlerin ekspresyon seviyeleri üzerindeki etkilerini belirlemeyi amaçlamıştır. 16 adet Wistar albino rat iki gruba ayrılarak 4 hafta boyunca farklı diyetlerle beslenmiştir. Birinci grup (Kontrol grubu) ad libitum beslenirken, diğer gruba günlük ihtiyacın yarısı (Yem Kısıtlama grubu, YK) kadar yem verilmiştir. Haftalık besin tüketimi ve vücut ağırlığı değişimlerinin yanı sıra beslenme periyodu sonrasında total kolesterol, HDL, LDL ve trigliserit düzeyleri belirlenmiştir. Yağ asidi profiline ek olarak karaciğerde FASN ve SCD-1 genlerinin ekspresyon seviyeleri ölçülmüştür. FR’nin ortalama vücut ağırlığı 7. günden sonra azalmaya başlarken, YK'de plazma glukoz seviyeleri kontrol grubuna kıyasla daha düşük bulunmuştur. YK grubunda FASN geni yaklaşık 6 kat artarken (P<0,05), SCD-1 önemsiz olacak şekilde yaklaşık 3 kat artmıştır. FR grubunda C15:0, C18:1 n9 trans, C18:2 n6 cis, C21:0, C20:2, C20:5 n3, n6 ve UFA miktarı kontrole göre daha düşükken, C16:0, C18:2 n6 trans, C20:3 n6, C22:6 n3, C22:1 n9, C22:2 ve SFA miktarının daha yüksek olduğu belirlenmiştir. Besin kısıtlamasının, maruz kalma süresi ve oranının dikkate alınmasına ek olarak, diğer metabolik organlardaki moleküler aktivite ve etkileşimler araştırılmalıdır.

Anahtar Kelimeler

• FASN
• karaciğer
• SCD-1
• yağ asitleri
• yem kısıtlama

Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats

Abstract

This study aimed to determine food restriction effects on the profile of fatty acids and major genes on lipogenesis expressions in liver. 16 Wistar albino rats were divided into two groups and different diets were given to groups for 4-weeks. First group was fed ad libitum (Control group), another group was fed the half amount of the daily requirement (Food Restriction group, FR). As well as weekly food consumption and body weight changes, total cholesterol, HDL, LDL, and triglyceride levels were determined at the end of the feeding period. In addition to the fatty acid profile, FASN and SCD-1 genes expression levels were measured in the liver. While the body weight averages decreased after 7 days and remained similar, plasma glucose levels were found lower in the FR. FASN was upregulated approximately 6 folds, and SCD-1 increased insignificantly about 3 folds in the FR. C15:0, C18:1 n9 trans, C18:2 n6 cis, C21:0, C20:2, C20:5 n3, n6 and UFA were lower, while C16:0, C18:2 n6 trans, C20:3 n6, C22:6 n3, C22:1 n9, C22:2 and SFA were higher in FR. In addition to considering the exposure time and rate of food restriction, molecular activity and interactions in other metabolic organs should be investigated.

Keywords

• FASN
• fatty acids
• food restiction
• liver
• SCD-1

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