Commercial Lavender (Lavandula Angustifolia) Oil’s Effects On Buck Spermatozoa During Cryopreservation

Abstract

The objective of this study was to assess the impact of lavender oil preparation on the spermatological data, oxidant-antioxidant parameters, and antimicrobial properties of frozen buck semen due to its antioxidant and antibacterial characteristics. To conduct a comprehensive analysis of these impacts, the study established nine experimental groups. Three control groups designed as; a negative control group, a group treated with 1000 IU/mL penicillin and 1000 µg/mL streptomycin (C1), and a group treated with 500 IU/mL penicillin and 500 µg/mL streptomycin (C2). Additionally, groups were created by adding lavender oil to the base diluent. Commercial lavender oil was diluted at a ratio of 10-4. We then formed four groups, each receiving different amounts of this diluted oil: 10 µL/mL, 15 µL/mL, 20 µL/mL, and 25 µL/mL. Simultaneously, two distinct groups were formed by using dosages of 20 µL/mL and 25 µL/mL by adding to the C2 group. The study revealed that lavender oil had protective effects on motility, plasma membrane integrity, and acrosome integrity. Furthermore, lavender oil's antioxidant properties positively affect frozen-thawed semen, and its antibacterial impact was shown when used in conjunction with the C2 group.

Keywords

• Lavender essential oil
• Buck semen
• Antioxidant
• Cryopreservation
• Lavandula angustifolia

Supporting Institution

None

Ethical Statement

The Scientific Ethical Committee of Kafkas University in Kars, Turkey has granted approval for all matters related to the experimental settings and assessment methodologies (2024-105).

Thanks

None

Ticari Lavanta (Lavandula Angustifolia) Yağının Kriyoprezervasyon Sırasında Teke Spermatozoası Üzerindeki Etkileri

Abstract

Bu çalışmada, lavanta yağı preparatının spermatolojik veriler, oksidan-antioksidan parametreler ve antioksidan ve antibakteriyel özellikleri nedeniyle dondurulmuş teke spermasının antimikrobiyal özellikleri üzerindeki etkisinin değerlendirmesi amaçlanmıştır. Bu etkilerin kapsamlı bir analizini yapmak için, çalışmada dokuz deney grubu oluşturulmuştur. Üç kontrol grubu; Negatif kontrol grubu, 1000 IU/mL penisilin ve 1000 ug/ml streptomisin (C1) içeren bir grup ve 500 IU/mL penisilin ve 500 ug/mL streptomisin (C2) içeren bir grup oluşturulmuştur. Ek olarak, baz sulandırıcı üzerine lavanta yağı ilave edilen gruplar oluşturulmuştur. Ticari lavanta yağı 10-4 oranında seyreltildi. Daha sonra her biri farklı lavanta yağı dozu içeren dört grup oluşturuldu: 10 uL/mL, 15 uL/ml, 20 uL/ml ve 25 uL/mL. Eşzamanlı olarak, C2 grubuna 20 uL/ml ve 25 uL/ml'lik dozlarda lavanta yağı ilave edilerek iki ayrı grup oluşturuldu. Çalışma, lavanta yağının motilite, plazma membran bütünlüğü ve akrozom bütünlüğü üzerinde koruyucu etkileri olduğunu ortaya koydu. Ayrıca, lavanta yağının antioksidan özellikleri dondurulmuş-çözdürülmüş teke sperması üzerine olumlu etkileri olduğu ve C2 grubu ile birlikte kullanıldığında gösterilmiştir antibakteriyel etkisinin olduğu görülmüştür.

Keywords

• Lavanta esansiyel yağı
• teke sperması
• antioksidan
• kriyoprezervasyon
• lavandula angustifolia

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