Commercial Lavender (Lavandula Angustifolia) Oil’s Effects On Buck Spermatozoa During Cryopreservation

Abstract

The objective of this study was to assess the impact of lavender oil preparation on the spermatological data, oxidant-antioxidant parameters, and antimicrobial properties of frozen buck semen due to its antioxidant and antibacterial characteristics. To conduct a comprehensive analysis of these impacts, the study established nine experimental groups. Three control groups designed as; a negative control group, a group treated with 1000 IU/mL penicillin and 1000 µg/mL streptomycin (C1), and a group treated with 500 IU/mL penicillin and 500 µg/mL streptomycin (C2). Additionally, groups were created by adding lavender oil to the base diluent. Commercial lavender oil was diluted at a ratio of 10-4. We then formed four groups, each receiving different amounts of this diluted oil: 10 µL/mL, 15 µL/mL, 20 µL/mL, and 25 µL/mL. Simultaneously, two distinct groups were formed by using dosages of 20 µL/mL and 25 µL/mL by adding to the C2 group. The study revealed that lavender oil had protective effects on motility, plasma membrane integrity, and acrosome integrity. Furthermore, lavender oil's antioxidant properties positively affect frozen-thawed semen, and its antibacterial impact was shown when used in conjunction with the C2 group.

Keywords

• Lavender essential oil
• Buck semen
• Antioxidant
• Cryopreservation
• Lavandula angustifolia

Ticari Lavanta (Lavandula Angustifolia) Yağının Kriyoprezervasyon Sırasında Teke Spermatozoası Üzerindeki Etkileri

Öz

Bu çalışmada, lavanta yağı preparatının spermatolojik veriler, oksidan-antioksidan parametreler ve antioksidan ve antibakteriyel özellikleri nedeniyle dondurulmuş teke spermasının antimikrobiyal özellikleri üzerindeki etkisinin değerlendirmesi amaçlanmıştır. Bu etkilerin kapsamlı bir analizini yapmak için, çalışmada dokuz deney grubu oluşturulmuştur. Üç kontrol grubu; Negatif kontrol grubu, 1000 IU/mL penisilin ve 1000 ug/ml streptomisin (C1) içeren bir grup ve 500 IU/mL penisilin ve 500 ug/mL streptomisin (C2) içeren bir grup oluşturulmuştur. Ek olarak, baz sulandırıcı üzerine lavanta yağı ilave edilen gruplar oluşturulmuştur. Ticari lavanta yağı 10-4 oranında seyreltildi. Daha sonra her biri farklı lavanta yağı dozu içeren dört grup oluşturuldu: 10 uL/mL, 15 uL/ml, 20 uL/ml ve 25 uL/mL. Eşzamanlı olarak, C2 grubuna 20 uL/ml ve 25 uL/ml'lik dozlarda lavanta yağı ilave edilerek iki ayrı grup oluşturuldu. Çalışma, lavanta yağının motilite, plazma membran bütünlüğü ve akrozom bütünlüğü üzerinde koruyucu etkileri olduğunu ortaya koydu. Ayrıca, lavanta yağının antioksidan özellikleri dondurulmuş-çözdürülmüş teke sperması üzerine olumlu etkileri olduğu ve C2 grubu ile birlikte kullanıldığında gösterilmiştir antibakteriyel etkisinin olduğu görülmüştür.

Anahtar Kelimeler

• Lavanta esansiyel yağı
• teke sperması
• antioksidan
• kriyoprezervasyon
• lavandula angustifolia

Kaynakça

1. Aktar A, Toker MB, Koca D, Uzun UC, Alcay S. (2024). The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen. Vet med sci 10(4):e1526. https://doi.org/10.1002/vms3.1526
2. Alcay S, Gokce E, Toker MB, Onder NT, Ustuner B, Uzabacı E, Gul Z, Cavus S. (2016). Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa. Cryobiology 72(3):269-273. https://doi.org/10.1016/j.cryobiol.2016.03.007
3. Anel-Lopez L, Riesco MF, Montes-Garrido R, Neila-Montero M, Boixo JC, Chamorro C, Ortega-Ferrusola C, Carvajal A, Altonaga JR, Paz P, Alvarez M, Anel L. (2021). Comparing the effect of different antibiotics in frozen-thawed ram sperm: is it possible to avoid their addition?. Front Vet Sci 8:656937. https://doi.org/10.3389/fvets.2021.656937
4. Avdatek F, Yeni D, İnanç ME, Çil B, Tuncer BP, Türkmen R, Taşdemir U. (2018). Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia 50(4):e12975. https://doi.org/10.1111/and.12975
5. Bucak MN, Bodu M, Başpınar N, Güngör Ş, İli P, Acibaeva B, Topraggaleh TR, Dursun Ş. (2019). Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Cell J 21(1):7-13. https://doi.org/10.22074/cellj.2019.5593
6. Cavanagh HM and Wilkinson JM. (2005). Lavender essential oil: a review. Australian Infection Control, 10:35-37. https://doi.org/10.1071/HI05035
7. Cornwell DG, Jones KH, Jiang Z, Lantry LE, Southwell‐Keely P, Kohar I, Thornton DE. (1998). Cytotoxicity of tocopherols and their quinones in drug‐sensitive and multidrug‐resistant leukemia cells. Lipids 33(3):295-301. J Am Oil Chem Soc https://doi.org/10.1007/s11745-998-0208-8
8. Danh LT, Han LN, Triet NDA, Zhao J, Mammucari R, Foster N. (2013). Comparison of chemical composition, antioxidant and antimicrobial activity of lavender (Lavandula angustifolia L.) essential oils extracted by supercritical CO 2, hexane and hydrodistillation Food Bioproc Tech 6:3481-3489. https://doi.org/10.1007/s11947-012-1026-z

9. Do DN, Nguyen DP, Tran TTA, Nguyen DQ, Lai NMT, Huynh TH, Do VM, Luu XC, Le XT. (2021). Evaluation of antibacterial properties of some essential oils from Vietnam. IOP Conf. Series: Materials Science and Engineering 1166(1):012040. https://doi.org/10.1088/1757-899X/1166/1/012040
10. Donelli D, Antonelli M, Bellinazzi C, Gensini GF, Firenzuoli F. (2019). Effects of lavender on anxiety: A systematic review and meta-analysis. Phytomedicine 65:153099. https://doi.org/10.1016/j.phymed.2019.153099
11. Falchi L, Pau S, Pivato I, Bogliolo L, Zedda MT. (2020). Resveratrol supplementation and cryopreservation of buck semen. Cryobiology 95:60-67. https://doi.org/10.1016/j.cryobiol.2020.06.005
12. Fismer KL and Pilkington K. (2012). Lavender and sleep: A systematic review of the evidence. Eur J Integr Med 4(4):e436-e447. https://doi.org/10.1016/j.eujim.2012.08.001
13. Ghanem N, Nasr NH, Abu Elnaga NA, Abou‐Hashim F, Kamel S, Warda M, Dessouki SM, AbdRabou, MA, Mehaisen GM. (2023). Molecular and physiochemical evaluation of buck semen cryopreserved with antioxidants. Reprod Domest Anim 58(6):813-822. https://doi.org/10.1111/rda.14354
14. Gürler H, Malama E, Heppelmann M, Calisici O, Leiding C, Kastelic J P, Bollwein H. (2016). Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm. Theriogenology 86(2):562-571. https://doi.org/10.1016/j.theriogenology.2016.02.007
15. İnanç ME, Avdatek F, Güngör Ş, Gülhan MF, Olğaç KT, Denk B, Yeni D, Taşdemir, U. (2023). Cryopreservation of ram semen using capsaicin supplemented tris extender. Kocatepe Vet J 16(2):166-173. https://doi.org/10.30607/kvj.1257228
16. Jadczak P, Kulpa D, Drozd R, Przewodowski W, Przewodowska A. (2020). Effect of AuNPs and AgNPs on the antioxidant system and antioxidant activity of lavender (Lavandula angustifolia Mill.) from in vitro cultures. Molecules 25(23):5511. https://doi.org/10.3390/molecules25235511
17. Khalique MA, Rehman H, Andrabi SMH, Majeed KA, Ahmad N, Fayyaz MH, Haider MS, Naz SS, Qureshi IZ, Sulaiman S. (2023). Antioxidant effects of zinc-oxide nanoparticles on post-thaw quality and in vivo fertility of Beetal buck spermatozoa. Small Rumin Res 225:107012. https://doi.org/10.1016/j.smallrumres.2023.107012
18. Kıvrak Ş. (2018). Essential oil composition and antioxidant activities of eight cultivars of Lavender and Lavandin from western Anatolia. Ind Crops Prod, 117:88-96. https://doi.org/10.1016/j.indcrop.2018.02.089
19. Kumar D, Khasatiya CT, Chaudhary SS, Singh VK. (2024). Evaluation of Antioxidant Potential of Taurine in Tris Egg Yolk Citrate Extender in Surti Buck Semen Preserved at Refrigerated Temperature. Asian J Dairy Food Res 43:95-99. https://doi.org/10.18805/ajdfr.DR-1936
20. Madeira EM, Goularte KL, Pradieé J, Mondadori RG, Lucia Jr T, Bianchi I, Vieira AD, Leite, FP. (2014). The use of antibiotics in cryopreservation of ram sperm. Int J Vet Med Res Reports 154947. https://doi.org/10.5171/2014.154947
21. Mocé ML, Esteve IC, Pérez-Fuentes S, Gómez EA, Mocé E. (2022). Microbiota in goat Buck ejaculates differs between breeding and non-breeding seasons. Front Vet Sci 9:867671. https://doi.org/10.3389/fvets.2022.867671
22. Moreira SS, Santos CS, Castelo TS, Bezerra LG, Praxedes ÉC, Matos TM, Souza-Junior JBF, Feijó FMC, Comizzoli P, Silva AR. (2022). Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries. Front Vet Sci 9:954921. https://doi.org/10.3389/fvets.2022.954921
23. Motlagh MK, Sharafi M, Zhandi M, Mohammadi-Sangcheshmeh A, Shakeri M, Soleimani M, Zeinoaldini S. (2014). Antioxidant effect of rosemary (Rosmarinus officinalis L.) extract in soybean lecithin-based semen extender following freeze–thawing process of ram sperm. Cryobiology 69(2):217-222. https://doi.org/10.1016/j.cryobiol.2014.07.007
24. Önder NT, Aktar A, Çakmak S, Çakmak İ, Toker MB, Alçay S. (2024). Insulin enhances drone semen quality after cryopreservation. J Apic Res 63(4):631-636. https://doi.org/10.1080/00218839.2024.2355039
25. Önder NT, Gökdemir T, Kilic MC, Şahin O, Yildiz S, Kacar C, Demir MCK, Öztürkler Y. (2023b). Insulin and bull sperm interactions during cryopreservation. Kafkas Univ Vet Fak Derg 29(4):401-5. https://doi.org/10.9775/kvfd.2023.29623
26. Önder NT, Gökdemir T, Kılıç MC, Yıldız S, Öztürkler Y. (2023a). Effects of Insulin Lispro on Ram Semen Cryopreservation. Kocatepe Vet J 16(3):439-443. https://doi.org/10.30607/kvj.1347383
27. Owen L, Laird K. (2018). Synchronous application of antibiotics and essential oils: dual mechanisms of action as a potential solution to antibiotic resistance. Crit Rev Microbiol 44(4):414-435. https://doi.org/10.1080/1040841X.2018.1423616
28. Placer ZA, Cushman LL, Johnson BC. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem 16:359-364. https://doi.org/10.1016/0003-2697(66)90167-9
29. Samuelson R, Lobl M, Higgins S, Clarey D, Wysong A. (2020). The effects of lavender essential oil on wound healing: A review of the current evidence. J Altern Complement Med 26:680-690. https://doi.org/10.1089/acm.2019.0286
30. Santos CS and Silva AR. (2020). Current and alternative trends in antibacterial agents used in mammalian semen technology. Anim Reprod 17:e20190111. https://doi.org/10.21451/1984-3143-AR2019-0111
31. Sedlak J, Lindsay RH. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 25:192-205.
32. Talya Bitkisel. June 11, 2024. Accessed June 11, 2024. https://www.talyabitkisel.com/certificates
33. Toker MB, Dogan I. (2023). Methionine, cysteine, and butylated hydroxytoluene enhance cryosurvival of ram semen on post-thaw and post-incubation time points. Trop Anim Health Prod 55(3):218. https://doi.org/10.1007/s11250-023-03642-z
34. Touazi L, Aberkane B, Bellik Y, Moula N, Iguer-Ouada M. (2018). Effect of the essential oil of Rosmarinus officinalis (L.) on rooster sperm motility during 4 C short-term storage. Vet World, 11(5), 590. https://doi.org/10.14202/vetworld.2018.590-597 Troisio I, Bertocchi M, Ventrella D, Scozzoli M, Di Vito M, Truzzi E, Benvenuti S, Mattarelli P, Bacci ML, Elmi A. (2024). Short-and long-term effects of essential oils on swine spermatozoa during liquid phase refrigeration. Sci Rep 14(1):285. https://doi.org/10.1038/s41598-023-51030-2
35. Üstüner B, Alcay S, Gokce E, Yılmaz M, Aktar A, Huraydin O, Duman M, Onder NT, Akal E, Nur Z. (2022). Lyophilized extender supplemented with rainbow trout (Oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm. Kafkas Univ Vet Fak Derg, 28(2):255-260. https://doi.org/10.9775/kvfd.2021.26855
36. Ustuner B, Ustuner H, Gokce E, Onder NT, Yilmaz MM, Huraydin O, Toker MB. (2023). The Combined Effect of Melatonin Implant and Removal of Buck Seminal Plasma on Cryopreservation During the Nonbreeding Season. Biopreserv Biobank 21(4):327-335. https://doi.org/10.1089/bio.2022.0074
37. Ustuner, B., Yılmaz MM, Huraydın O, Cimen T, Ak Sonat F, Aktar A, Gokce E, Karakci D, Sengul E, Soylu MK, Nur Z (2024). Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation. J Hellenic Vet Med Soc 75(2):7361-7370. https://doi.org/10.12681/jhvms.34504
38. Valdivieso-Ugarte M, Gomez-Llorente C, Plaza-Díaz J, Gil Á. (2019). Antimicrobial, antioxidant, and immunomodulatory properties of essential oils: A systematic review. Nutrients 11(11):2786. https://doi.org/10.3390/nu11112786
39. Yang SA, Jeon SK, Lee EJ, Shim CH, Lee IS. (2010). Comparative study of the chemical composition and antioxidant activity of six essential oils and their components. Nat Prod Res 24(2):, 140-151. https://doi.org/10.1080/14786410802496598
40. Yildiz S, Ozturkler Y, Ari UC, Lehimcioglu NC, Atakisi E, Kulaksiz R. (2015). The effects of L-Ergothioneine, Nacetylcystein and cystein on freezing of ram semen. Kafkas Univ Vet Fak Derg 21:81–86. https://doi.org/10.9775/kvfd.2014.11792
41. Zych S, Adaszyńska-Skwirzyńska M, Szewczuk MA, Szczerbińska D. (2024). Interaction between Enrofloxacin and Three Essential Oils (Cinnamon Bark, Clove Bud and Lavender Flower)—A Study on Multidrug-Resistant Escherichia coli Strains Isolated from 1-Day-Old Broiler Chickens. Int J Mol Sci 25(10):5220. https://doi.org/10.3390/ijms25105220