Effect of Melatonin on Development, Survival Rate and Immune Response in the Juveniles of Freshwater Crayfish (Pontastacus leptodactylus Eschscholtz, 1823)

Year 2021, Volume: 7 Issue: 2, 108 - 114, 26.08.2021

Remziye Özkök , Mevlüt Aktaş , Kamile Erol , Behıre Dıdınen , Mustafa Ceylan , Soner Çetinkaya , Oğuz Yaşar Uzunmehmetoğlu Şakir Çınar , Nuran Cavdar , Mehmet Pazar

https://doi.org/10.17216/limnofish.761841

https://izlik.org/JA68SA49DN

Abstract

The aim of this study was to investigate the effects of melatonin on growth, survival and immune response (total hemocyte counts) of Pontastacus leptodactylus juveniles (stage II-IV). With this aim, five separate groups with 4 replicates were formed by adding melatonin at the doses of 0, 0.5, 1, 2, 4 mg/kg to the trial feed. The experiments was conducted using stage II P. leptodactylus juveniles which fed for 60 days. During the experiments, a 50% water change once a day and continuous ventilation was provided. Stock density was determined as 20 juveniles for each replicate tank. The juveniles were fed at rate of 5% of their body weight once a day. The average initial weight and total length of juveniles were 34 ± 4 mg and 11.6 ± 0.4 mm, repectively. At the end of the experiment, the maximum average total length (22.0 ± 3.6 mm) was in the group fed with 2 mg/kg melatonin containing diet, the best average live weight (216 ± 116 mg) was in the group fed with 4 mg/kg melatonin containing diet, the highest survival rate (44.4 ± 13.9%) was in the 2 mg/kg melatonin group and the highest average total hemocyte count (2.27 ± 0.49 x 106 cells/ml) was in the 2 mg/kg melatonin group. However, the differences between the groups were not statistically significant (p > 0.05). As a result of this study, it was determined that feeding with melatonin supplemented feed have no significant effect on growth, survival and immune response of P. leptodactylus juveniles.

Keywords

crayfish , pontastacus leptodactylus , melatonin , survival rate , growth

Project Number

TAGEM/HAYSUD/2015/A11/P-01/3

Melatonin’in Tatlısu İstakozu (Pontastacus leptodactylus Eschscholtz, 1823) Yavrularında Gelişim, Hayatta Kalma Oranı ve Bağışıklık Yanıtı Üzerindeki Etkisi

https://izlik.org/JA68SA49DN

Abstract

Bu çalışmanın amacı melatoninin, Pontastacus leptodactylus yavrularının (II-IV. dönem) gelişim, hayatta kalma oranı ve bağışıklık yanıtı (total hemosit sayısı) üzerindeki etkilerini araştırmaktır. Melatonin dozları 0, 0,5, 1, 2, 4 mg / kg oranında deneme yemine ilave edilerek 4 tekerrürlü 5 ayrı grup şeklinde deneme grupları oluşturulmuştur. Denemeler, II. Dönem P. leptodactylus yavruları ile kurulmuş ve 60 gün süre ile besleme yapılmıştır. Deneme süresince günde bir kez %50 oranında su değişimi ve sürekli bir havalandırma sağlanmıştır. Stok yoğunluğu her deneme tekerrürü için 20 yavru olarak belirlenmiştir. Yavrular, günde bir kez canlı ağırlıklarının %5’i oranında beslenmiştir. Yavruların başlangıç ortalama ağırlık ve total boyları 34 ± 4 mg ve 11,6 ± 0,4 mm olarak belirlenmiştir. Deneme sonunda en yüksek ortalama total boy 22,0 ± 3,6 mm olarak 2 mg/kg melatonin içeren grupta, en iyi ortalama canlı ağırlık 216 ± 116 mg olarak 4 mg/kg melatonin grubunda, en iyi yaşama oranı %44,4 ± 13,9 olarak 2 mg / kg melatonin doz grubunda ve en yüksek ortalama total hemosit miktarı ise 2,27 ± 0,49 x 106 hücre/ml olarak yine 2 mg/kg melatonin doz grubunda elde edilmiştir. Bununla birlikte gruplar arasındaki farklar istatistiki açıdan önemli bulunmamıştır (p > 0,05). Sonuç olarak, bu çalışmada
P. leptodactylus yavrularının gelişim, hayatta kalma oranı ve bağışıklık yanıtı üzerinde melatonin katkılı yemler ile beslemenin önemli bir etkisinin olmadığı belirlenmiştir.

Keywords

kerevit , pontastacus leptodactylus , malatonin , hayatta kalma oranı , gelişim

Supporting Institution

TAGEM

Project Number

TAGEM/HAYSUD/2015/A11/P-01/3

Thanks

Bu çalışmayı destekleyen (TAGEM / HAYSUD / 2015 / A11 / P-01/3 projesi), Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü’ne teşekkür ediyoruz.

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