Boğa sperması motilite parametreleri üzerine dietilheksil fitalatın etkisi

Öz

Dietilheksil fitalat, sert yapılı plastiklere esneklik katmak amacıyla dünya genelinde yaygın bir şeklide kullanılan, çevresel toksik bir kimyasaldır. İçerisinde bulunduğu ve gevşek bağlarla tutunduğu ürünlerden kolayca ayrılarak çevreye yayılan dietilheksil fitalat sindirim, solunum ve deri teması yollarıyla insan ve hayvanların vücuduna geçmekte ve çeşitli toksik etkilere neden olmaktadır. Bu çalışmanın amacı, dietilheksil fitalatın boğa sperması üzerine in vitro toksik etkisini, bilgisayarlı sperm analiz sistemi ile araştırmaktır. Boğalardan suni vajen ile elde edilen spermalar, fosfat tampon solüsyonu ile 50x10⁶/ml spermatozoa olacak şekilde sulandırıldı ve dimetil sülfoksitte çözdürülen dietilheksil fitalatın 0, 1, 10, 100, 250 ve 500 µg/ml dozlarına maruz bırakılarak 1, 2, 3 ve 4 saat süreyle 37 °C su banyosu içerisinde inkübe edildi. Her inkübasyon süresinin sonunda kontrol ve deneme gruplarından alınan sperma örneklerinin motilite parametreleri analiz edildi. Yapılan değerlendirme sonucunda, dietilheksil fitalatın çalışmada kullanılan düşük dozlarında, doğrudan spermatozoa üzerine toksik etkisi tespit edilemezken, bu etkinin yüksek konsantrasyonlarda zamanla ve doza bağlı olarak ortaya çıktığı belirlendi. Özellikle 500 µg/ml dietilheksil fitalat maruziyetinin sperma hareketlilik parametrelerinden, ortalama yol hızı ve doğrusal hızı 2. saatten itibaren, total motilite ve progresif motiliteyi ise 3. saatten itibaren kontrol gruplarına göre önemli derecede azalttığı belirlendi (p<0.05). İn vitro olarak elde edilen sonuçlar dietilheksil fitalatın erkek üreme organlarında toksik birikimine bağlı olarak boğalarda spermatogenezisi etkiliye bileceğini yönündedir.

Anahtar Kelimeler

• Boğa,CASA,DEHP,Motilite,Sperma

Effect of diethylhexyl phthalate on sperm motility parameters in bull

Öz

Diethylhexyl Phthalate is an environmentally toxic chemical commonly used in worldwide to make rigid plastics flexible. Due to its loosely bonded to plastic products, diethylhexyl phthalate easily leaches into the environment. After humans and animals exposure to diethylhexyl phthalate via digestion, respiration and skin contact, it causes various toxic effects in the body. The aim of this study was to investigate the in vitro toxic effect of diethylhexyl phthalate on bull semen motility via a computerized sperm analysis system. Semen collected from the bulls with artificial vagina were diluted with phosphate buffer solution to 50x10⁶/ml spermatozoa and incubated in a 37 ° C water bath for 1, 2, 3 and 4 h by exposing to 0, 1, 10, 100, 250 and 500 µg/ml doses of diethylhexyl phthalate dissolved in dimethyl sulfoxide. At the end of each incubation period, the motility parameters of semen samples taken from control and experimental groups were analyzed. As a result of the evaluation, the toxic effects of diethylhexyl phthalate on the spermatozoa were not determined at low doses used in this study, but this effect occurred at high concentrations by the time. It was observed that exposure of diethylhexyl phthalate particularly at the doses of 500 µg/ml significantly decreased the velocity of average path and linear velocity parameters after 2 h, total motility and progressive motility parameters after 3 h compared to control groups (p<0.05). The in vitro results suggest that toxic accumulation of diethylhexyl phthalate in male reproductive organs can affect fertility in bovine.

Anahtar Kelimeler

• Bull,CASA,DEHP,Motility,Sperm

Kaynakça

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