Konya ve Aydın İllerinde Çilekte Enfeksiyon Oluşturan Bazı Önemli Virüslerin Belirlenmesi
Yıl 2020,
, 34 - 37, 03.06.2020
Handan Çulal Kılıç
,
Raziye Yetik
Selda Işık
Öz
Bu çalışma, 2017 üretim yılı Konya ve Aydın
İlleri çilek üretim alanlarında yürütülmüştür. Survey alanlarında virüs
belirtisi gösteren 92 yaprak örneği toplanmıştır. Toplanan örneklerde Strawberry latent ringspot virus (SLRSV)
ve Strawberry mild yellow edge virus (SMYEV)’ lerinin varlığını belirlemek
amacıyla DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunosorbent Assay)
yöntemi kullanılmıştır. DAS-ELISA testi sonucunda 92 örnekten 38 adedinin
virüsler ile enfekteli olduğu tespit edilmiştir. Elde edilen sonuçlara göre; 4
örnekte SLRSV, 29 örnekte SMYEV, 5 örnekte de
karışık enfeksiyon ortaya konulmuştur.
Kaynakça
- Anonim (2017). http://faostat.fao.org/site/567/. (22 Mayıs 2017).
- Anonim, 2018. http://www.tuik.gov.tr/ (11 Mart 2018).
- Atasay, A., Türemiş, N. (2008). Eğirdir (Isparta) koşullarında organik çilek yetiştiriciliğinin uygulanabilirliği üzerine bir araştırma. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Dergisi 18(3): 72-81.
- Clark, M.F., Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 475-483.
- Dara, S.K. (2015). Virus decline of strawberry in California and the role of insect vectors and associated viruses. Plant Health Progress Doi:10.1094/Php-mr-15-0023.
- El-Morsy, Sh.I., El-Sheikh, M.A., Abd El-Razik, R.A., Youssef, Sahar A., Shalaby, A.A. (2017). Molecular identification of Strawberry Latent Ring Spot Virus (SLRSV) in Egypt. Journal of Basic and Environmental Sciences 4: 24-33.
- Hancock, J.F., Sjulin, T.M., Lobos, G.A. (2008). Strawberries. In: Temperate Fruit Crop Breeding, Germplasm to Genomics Chapter: Hancock, J.F. (eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, 1-37.
- Handa, A., Sharma, A. (2018). Incidence of major virus diseases of strawberry in north western Himalayan region of India. Journal of Pharmacognosy and Phytochemistry 7(5): 245-248.
- İkinci, A., Türemiş, N., Bolat, I. (2017). Çilek yetiştiriciliğinde ve çilek ıslahında güncel yaklaşımlar. Tarım Türk Dergisi 60-64.
Ma, X., Cui, H., Bernardy, M., Tian L.,, Abbasi, P., Wang, A. (2015). Molecular characterization of a Strawberry mild yellow edge virus isolate from Canada. Canadian Journal of Plant Pathology, 37(3): 369-375.
- Martin, R.R., Tzanetakis, I.E. (2006). Characterization and recent advances in detection of strawberry viruses. Plant Diseases 90:384-396.
- Sarıgül, T. (2013). Düzce İli Çilek Üretim Alanlarında Görülen Fungal Hastalıkların Belirlenmesi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Yüksek Lisans Tezi, 57.
- Sedlák, J., Paprštein, F., Suchá, J. (2019): Influence of chemotherapy on development and production of virus free in vitro strawberry plants. Horticultural Science (Prague), 46: 53-56.
- Sharma, A., Handa, A., Kapoor, S., Shylla, B. (2018). First report of Strawberry mild yellow edge virus in India. International Journal of Chemical Studies 6(3): 662-664.
- Yeşilçöllü, S., Gümüş, M., Paylan, I.C. (2011). Studies on the detection of viruses in strawberry growing areas in Aegean region. The Journal of Turkish Phytopathology 40(1-3): 13-20.
Detection of Some Important Viruses Infecting in Konya and Aydın Provinces
Yıl 2020,
, 34 - 37, 03.06.2020
Handan Çulal Kılıç
,
Raziye Yetik
Selda Işık
Öz
This study was carried out in strawberry production areas in Konya and
Aydın provinces in 2017. In the surveys; the total of 92 leaf samples were
collected from strawberry plants with virus like symptoms. Collected samples
were tested by DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunosorbent
Assay) to determine the presence of Strawberry
latent ringspot virus (SLRSV) and Strawberry
mild yellow edge virus (SMYEV). According to DAS-ELISA tests; It was
determined that 38 samples out of 92 were infected with viruses. In this
results; SLRSV in 4 samples and SMYEV in 29 samples were found. 5 samples were
infected with two viruses.
Kaynakça
- Anonim (2017). http://faostat.fao.org/site/567/. (22 Mayıs 2017).
- Anonim, 2018. http://www.tuik.gov.tr/ (11 Mart 2018).
- Atasay, A., Türemiş, N. (2008). Eğirdir (Isparta) koşullarında organik çilek yetiştiriciliğinin uygulanabilirliği üzerine bir araştırma. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Dergisi 18(3): 72-81.
- Clark, M.F., Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 475-483.
- Dara, S.K. (2015). Virus decline of strawberry in California and the role of insect vectors and associated viruses. Plant Health Progress Doi:10.1094/Php-mr-15-0023.
- El-Morsy, Sh.I., El-Sheikh, M.A., Abd El-Razik, R.A., Youssef, Sahar A., Shalaby, A.A. (2017). Molecular identification of Strawberry Latent Ring Spot Virus (SLRSV) in Egypt. Journal of Basic and Environmental Sciences 4: 24-33.
- Hancock, J.F., Sjulin, T.M., Lobos, G.A. (2008). Strawberries. In: Temperate Fruit Crop Breeding, Germplasm to Genomics Chapter: Hancock, J.F. (eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, 1-37.
- Handa, A., Sharma, A. (2018). Incidence of major virus diseases of strawberry in north western Himalayan region of India. Journal of Pharmacognosy and Phytochemistry 7(5): 245-248.
- İkinci, A., Türemiş, N., Bolat, I. (2017). Çilek yetiştiriciliğinde ve çilek ıslahında güncel yaklaşımlar. Tarım Türk Dergisi 60-64.
Ma, X., Cui, H., Bernardy, M., Tian L.,, Abbasi, P., Wang, A. (2015). Molecular characterization of a Strawberry mild yellow edge virus isolate from Canada. Canadian Journal of Plant Pathology, 37(3): 369-375.
- Martin, R.R., Tzanetakis, I.E. (2006). Characterization and recent advances in detection of strawberry viruses. Plant Diseases 90:384-396.
- Sarıgül, T. (2013). Düzce İli Çilek Üretim Alanlarında Görülen Fungal Hastalıkların Belirlenmesi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Yüksek Lisans Tezi, 57.
- Sedlák, J., Paprštein, F., Suchá, J. (2019): Influence of chemotherapy on development and production of virus free in vitro strawberry plants. Horticultural Science (Prague), 46: 53-56.
- Sharma, A., Handa, A., Kapoor, S., Shylla, B. (2018). First report of Strawberry mild yellow edge virus in India. International Journal of Chemical Studies 6(3): 662-664.
- Yeşilçöllü, S., Gümüş, M., Paylan, I.C. (2011). Studies on the detection of viruses in strawberry growing areas in Aegean region. The Journal of Turkish Phytopathology 40(1-3): 13-20.