Molecular Identification of Yeasts Isolated from Thermophilic Environment in Aydın Province

Year 2017, Volume 8, Issue 1, 48 - 59, 28.04.2017

H. Halil BIYIK Esin POYRAZOĞLU ÇOBAN Yusuf GEROĞLU İlgıt KIRGIZ

Abstract

For the isolation of thermophilic yeasts, soil and water samples around of Bereket geothermal plants in Buharkent district of Aydin province were used as material. As a result of the obtained data and analyzes, it was determined that certain strains showed homologies with the regions of ITS1 and ITS2 at the rates of 98% and 99% of the isolates. These species are registered in GenBank Saccharomyces cerevisiae strain levule 23, Candida albicans, Candida albicans strain m74a, Pichia jadinii strain ATCC 22023, Saccharomyces cerevisiae, Kluyveromyces marxianus strain P2, Cyberlindnera jadinii strain h79b and Candida glabrata strain 86A are identified as yeast species.

Keywords

Soil, Water, Thermophilic yeast, Isolation, ITS, Phylogenetic analysis

Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı

Abstract

Termofilik mayaların izolasyonu için Aydın ili Buharkent ilçesi Bereket Jeotermal tesislerinin çevresindeki toprak ve su örnekleri materyal olarak kullanılmıştır. Elde edilen veriler ve analizler sonucunda, izolatların %98 ve %99 gibi değişen oranlarda belirli türlerinin ITS1 ve ITS2 bölgeleriyle homoloji gösterdiği tespit edilmiştir.  Bu türlerin GenBank’ta kayıtlı Saccharomyces cerevisiae strain levure 23, Candida albicans, Candida albicans strain m74a, Cyberlindnera jadinii strain ATCC 22023, Saccharomyces cerevisiae, Kluyveromyces marxianus strain P2, Pichia manshurica strain h79b, Candida glabrata strain 86A adlı maya suşları olduğu belirlenmiştir.

Keywords

Toprak, Su, Termofilik maya, İzolasyon, ITS, Filogenetik analiz

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