Molecular Identification of Yeasts Isolated from Thermophilic Environment in Aydın Province

Year 2017, Volume: 8 Issue: 1, 48 - 59, 28.04.2017

H. Halil Bıyık , Esin Poyrazoğlu Çoban Yusuf Geroğlu İlgıt Kırgız

Abstract

For
the isolation of thermophilic yeasts, soil and water samples around of Bereket
geothermal plants in Buharkent district of Aydin province were used as
material. As a result of the obtained data and analyzes, it
was determined that certain strains showed homologies with the regions of ITS1
and ITS2 at the rates of 98% and 99% of the isolates. These
species are registered in GenBank Saccharomyces cerevisiae strain levule 23, Candida albicans,
Candida albicans strain m74a,
Pichia jadinii strain
ATCC 22023, Saccharomyces cerevisiae, Kluyveromyces marxianus strain P2, Cyberlindnera jadinii strain h79b and Candida glabrata strain 86A are identified as yeast
species.

Keywords

Soil, Water, Thermophilic yeast, Isolation, ITS, Phylogenetic analysis

Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı

Abstract

Termofilik
mayaların izolasyonu için Aydın ili Buharkent ilçesi Bereket Jeotermal
tesislerinin çevresindeki toprak ve su örnekleri materyal olarak
kullanılmıştır. Elde edilen veriler ve analizler sonucunda, izolatların %98 ve
%99 gibi değişen oranlarda belirli türlerinin ITS1 ve ITS2 bölgeleriyle
homoloji gösterdiği tespit edilmiştir. 
Bu türlerin GenBank’ta kayıtlı Saccharomyces cerevisiae strain levure 23, Candida albicans,
Candida albicans strain
m74a, Cyberlindnera jadinii strain ATCC 22023, Saccharomyces
cerevisiae, Kluyveromyces marxianus strain P2, Pichia manshurica strain h79b, Candida glabrata strain 86A adlı maya suşları olduğu belirlenmiştir.

Keywords

Toprak, Su, Termofilik maya, İzolasyon, ITS, Filogenetik analiz

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