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Molecular Identification of Yeasts Isolated from Thermophilic Environment in Aydın Province

Yıl 2017, Cilt 8, Sayı 1, 48 - 59, 28.04.2017

H. Halil BIYIK Esin POYRAZOĞLU ÇOBAN Yusuf GEROĞLU İlgıt KIRGIZ

Öz

For the isolation of thermophilic yeasts, soil and water samples around of Bereket geothermal plants in Buharkent district of Aydin province were used as material. As a result of the obtained data and analyzes, it was determined that certain strains showed homologies with the regions of ITS1 and ITS2 at the rates of 98% and 99% of the isolates. These species are registered in GenBank Saccharomyces cerevisiae strain levule 23, Candida albicans, Candida albicans strain m74a, Pichia jadinii strain ATCC 22023, Saccharomyces cerevisiae, Kluyveromyces marxianus strain P2, Cyberlindnera jadinii strain h79b and Candida glabrata strain 86A are identified as yeast species.

Anahtar Kelimeler

Soil, Water, Thermophilic yeast, Isolation, ITS, Phylogenetic analysis

Kaynakça

  • Abdel-Sater M.A., Moubasher A.A., Soliman Z. Identification of three yeast species using the conventional and internal transcribed spacer region sequencing methods as first or second global record from human superficial infections, Mycoses, 59(10) 652-661 (2016).
  • Anonim. Kaplıcaya Sahip Belediyeler Birliği (2001).
  • Arthur H., Watson K. Thermal adaptation in yeast: growth temperatures, membrane lipid, and cytochrome composition of psychrophilic, mesophilic, and thermophilic yeasts, Journal of Bacteriology, 128 56–68 (1976).
  • Baker G.C., Gaffer S., Cowan A.D., Suharto A.R. Bacterial community analysis of Indonesian hot springs, FEMS Microbiology Letters, 200 103-109 (2001).
  • Charles P.E, Dalle F., Aube H., Doise J.M, Quenot J.P, Aho L.S, Chavanet P., Blettery B. Candida spp. colonization significance in critically ill medical patients: a prospective study, Intensive Care Medicine, 31(3) 393-400 (2005).
  • Choi G.W., Umb H.J., Kim Y., Kang H.w., Kim M., Chung B.W., Kim Y.H. Isolation and characterization of two soil derived yeasts for bioethanol production on Cassava starch, Biomass and Bioenergy, 34(8) 1223-1231 (2010).
  • Deacon J. The microbial world: Thermophilic microorganisms produced by institute of Cell and Molecular Biology. The University of Edinburgh, (http://helios.bto.ed.ac.uk/bto/microbes/thermo.htm.), (2007).
  • Dlauchy D., Tornai-Lehoczki J., Peter G. Restriction enzyme analysis of PCR amplified rDNA as a taxonomic tool in yeast identification, Systematic and Applied Microbiology, 22(3) 445-53 (1999).
  • El-Latif Hesham A., Wambui V., Henry Ogola J.O., Maina J.M. Phylogenetic analysis of isolated biofuel yeasts based on 5.8S-ITS rDNAand D1/D2 26S rDNA sequences, Journal of Genetic Engineering and Biotechnology, 12(1) 37-43 (2014).
  • Fernandez-Espinar M.T, Martorell R., De Llanos R., Querol A. Molecular methods to identify and characterize yeasts in food sand beverages. In: Yeasts in food and beveragesed: Querol A. and Fleet G (eds), Springer-Verlag, Berlin, Germany, p. 55-82, (2006).
  • Fitzpatric D.A., Logue M.E., Stajich J.E., Butler G. A fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis, BMC Evolutionary Biology, 6(99) 1-15 (2006).
  • Genç T.T., Çıldır İ.N. Bozcaada üzüm çeşitleri üzerinde Non-Saccharomyces mayaların dağılımı, Biyoloji Bilimleri Araştırma Dergisi, 5(1) 115-120 (2012).
  • Gonz´alez-Hern´andez J.C., Perez E., Damian R.M., Chavez-Parga M.C. Isolation, molecular and fermentative characterization of a yeast used in ethanol production during mezcal elaboration, Revista Mexicana de Ingeniería Química, 11(3) 389-400 (2012).
  • Hierro A., Sun J., Rusnak A.S., Kim J., Prag G., Emr S.D., Hurley J.H. Structure of the ESCRT-II endosomal trafficking complex, Nature, 431(7005) 221-225 (2004).
  • Hoffman C.S., Winston F. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for trans formation of Escherichia coli, Gene, 57(2) 267–272 (1987).
  • Hundson H.J. Fungal Biology. Edward Amold Press, London, 6 158-75 (1987).
  • Kurtzman C.P., Robnett C.J. Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species, FEMS Yeast Research, 13(1) 23-33 (2013).
  • Madigan MT, Martinko JM, Parker J. Brock biology of microorganisms, 14th edn. Pearson Education Limited (2015). Lee B.H. Fundamentals of food biotechnolology, VCH Publishers, USA, p. 431, (1996).
  • Lopes M.B, Soden A., Martens A., Henschke P.A, Langridge P. Differentiation and species identification of yeasts using PCR, International Journal of Systematic Bacteriology, 48(1) 279-286 (1998).
  • Pfaller M.A., Diekema D.J. Rare and emerging opportunistic fungal pathogens: Concern for resistance beyond Candida albicans and Aspergillus fumigatus, Journal of Clinical Microbiology, 42(10) 4419-4431 (2004).
  • Redman R.S, Litvintseva A., Sheehan K.B., Henson J.M., Rodringuez R.J. Fungi from geothermal soils in Yellowstone National Park. Applied and Environmental Microbiology, 65 5193-7 (1999).
  • Schoch C.L., Seifert K.A., Huhndorf S., Robert V., Spouge J.L., Levesque C.A., Chen W., Consortium F.B. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi, Pnas, 109(16) 6241–6246 (2012).
  • Senses-Ergul Ş., Agoston R., Belak A., Deak T. Characterization of some yeasts isolated from foods by traditional and molecular tests, International Journal of Food Microbiology, 108(1) 120-124 (2006).
  • Shin K.S, Shin Y.K, Yoon J.H, Park Y.H. Candida thermophila sp. nov., a novel thermophilic yeast isolated from soil, International Journal of Systematic Evolutionary Microbiology, 51 2167–2170, (2001).
  • Šuranská H., Raspor P., Uroić K., Golić N., Kos B., Mihajlović S., Begović J., Šušković J., Topisirović L., Čadež N. Characterisation of the yeast and mould biota in traditional white pickled cheeses by culture-dependent and independent molecular techniques, Folia Microbiologica (Praha), 61(6) 455-463 (2016).
  • Tofalo R., Chaves-López C., DiFabio F., Schirone M., Felis G.E., Torriani S., Paparella A., Suzzi G. Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must, International Journal of Food Microbiology, 130(3) 179-187 (2009).
  • Van den Burg B. Extremophiles as a source for novel enzymes, Current Opinion Microbiology, 6(3), 213-218 (2003).
  • Van Der Vossen J.M.B.M., Rahaoui H., De Nijs M.W.C.M.,Hartog B.J. PCR methods for tracing and detection of yeasts in the food chain. In: Yeasts in Food, Boekhout T, Robert V (eds), BayerleinGmbH, Neusass, Germany, p. 123-137, (2003).
  • Vaughan-Martini A. Reflections on the clasification of yeasts for different end-users in biotechnology, ecology, and medicine, International Microbiology, 6(3) 175-182 (2003).
  • White T.J., Bruns T., Lee S., Taylor J.W. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A.,Gelfand, D.H., Snissky, J.J., White, T.J. (Eds.), PCR protocols: A guide to method sand applications. AcademicPressInc.,NewYork, p. 315–322, (1990).
  • Zhang Y., Wu W.P., Hu D.M., Su Y.Y., Cai L. A new thermophilic species of Myceliophthora from China, Mycological Progress, 13(1)165–170 (2014).

Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı

Yıl 2017, Cilt 8, Sayı 1, 48 - 59, 28.04.2017

H. Halil BIYIK Esin POYRAZOĞLU ÇOBAN Yusuf GEROĞLU İlgıt KIRGIZ

Öz

Termofilik mayaların izolasyonu için Aydın ili Buharkent ilçesi Bereket Jeotermal tesislerinin çevresindeki toprak ve su örnekleri materyal olarak kullanılmıştır. Elde edilen veriler ve analizler sonucunda, izolatların %98 ve %99 gibi değişen oranlarda belirli türlerinin ITS1 ve ITS2 bölgeleriyle homoloji gösterdiği tespit edilmiştir.  Bu türlerin GenBank’ta kayıtlı Saccharomyces cerevisiae strain levure 23, Candida albicans, Candida albicans strain m74a, Cyberlindnera jadinii strain ATCC 22023, Saccharomyces cerevisiae, Kluyveromyces marxianus strain P2, Pichia manshurica strain h79b, Candida glabrata strain 86A adlı maya suşları olduğu belirlenmiştir.

Anahtar Kelimeler

Toprak, Su, Termofilik maya, İzolasyon, ITS, Filogenetik analiz

Kaynakça

  • Abdel-Sater M.A., Moubasher A.A., Soliman Z. Identification of three yeast species using the conventional and internal transcribed spacer region sequencing methods as first or second global record from human superficial infections, Mycoses, 59(10) 652-661 (2016).
  • Anonim. Kaplıcaya Sahip Belediyeler Birliği (2001).
  • Arthur H., Watson K. Thermal adaptation in yeast: growth temperatures, membrane lipid, and cytochrome composition of psychrophilic, mesophilic, and thermophilic yeasts, Journal of Bacteriology, 128 56–68 (1976).
  • Baker G.C., Gaffer S., Cowan A.D., Suharto A.R. Bacterial community analysis of Indonesian hot springs, FEMS Microbiology Letters, 200 103-109 (2001).
  • Charles P.E, Dalle F., Aube H., Doise J.M, Quenot J.P, Aho L.S, Chavanet P., Blettery B. Candida spp. colonization significance in critically ill medical patients: a prospective study, Intensive Care Medicine, 31(3) 393-400 (2005).
  • Choi G.W., Umb H.J., Kim Y., Kang H.w., Kim M., Chung B.W., Kim Y.H. Isolation and characterization of two soil derived yeasts for bioethanol production on Cassava starch, Biomass and Bioenergy, 34(8) 1223-1231 (2010).
  • Deacon J. The microbial world: Thermophilic microorganisms produced by institute of Cell and Molecular Biology. The University of Edinburgh, (http://helios.bto.ed.ac.uk/bto/microbes/thermo.htm.), (2007).
  • Dlauchy D., Tornai-Lehoczki J., Peter G. Restriction enzyme analysis of PCR amplified rDNA as a taxonomic tool in yeast identification, Systematic and Applied Microbiology, 22(3) 445-53 (1999).
  • El-Latif Hesham A., Wambui V., Henry Ogola J.O., Maina J.M. Phylogenetic analysis of isolated biofuel yeasts based on 5.8S-ITS rDNAand D1/D2 26S rDNA sequences, Journal of Genetic Engineering and Biotechnology, 12(1) 37-43 (2014).
  • Fernandez-Espinar M.T, Martorell R., De Llanos R., Querol A. Molecular methods to identify and characterize yeasts in food sand beverages. In: Yeasts in food and beveragesed: Querol A. and Fleet G (eds), Springer-Verlag, Berlin, Germany, p. 55-82, (2006).
  • Fitzpatric D.A., Logue M.E., Stajich J.E., Butler G. A fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis, BMC Evolutionary Biology, 6(99) 1-15 (2006).
  • Genç T.T., Çıldır İ.N. Bozcaada üzüm çeşitleri üzerinde Non-Saccharomyces mayaların dağılımı, Biyoloji Bilimleri Araştırma Dergisi, 5(1) 115-120 (2012).
  • Gonz´alez-Hern´andez J.C., Perez E., Damian R.M., Chavez-Parga M.C. Isolation, molecular and fermentative characterization of a yeast used in ethanol production during mezcal elaboration, Revista Mexicana de Ingeniería Química, 11(3) 389-400 (2012).
  • Hierro A., Sun J., Rusnak A.S., Kim J., Prag G., Emr S.D., Hurley J.H. Structure of the ESCRT-II endosomal trafficking complex, Nature, 431(7005) 221-225 (2004).
  • Hoffman C.S., Winston F. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for trans formation of Escherichia coli, Gene, 57(2) 267–272 (1987).
  • Hundson H.J. Fungal Biology. Edward Amold Press, London, 6 158-75 (1987).
  • Kurtzman C.P., Robnett C.J. Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species, FEMS Yeast Research, 13(1) 23-33 (2013).
  • Madigan MT, Martinko JM, Parker J. Brock biology of microorganisms, 14th edn. Pearson Education Limited (2015). Lee B.H. Fundamentals of food biotechnolology, VCH Publishers, USA, p. 431, (1996).
  • Lopes M.B, Soden A., Martens A., Henschke P.A, Langridge P. Differentiation and species identification of yeasts using PCR, International Journal of Systematic Bacteriology, 48(1) 279-286 (1998).
  • Pfaller M.A., Diekema D.J. Rare and emerging opportunistic fungal pathogens: Concern for resistance beyond Candida albicans and Aspergillus fumigatus, Journal of Clinical Microbiology, 42(10) 4419-4431 (2004).
  • Redman R.S, Litvintseva A., Sheehan K.B., Henson J.M., Rodringuez R.J. Fungi from geothermal soils in Yellowstone National Park. Applied and Environmental Microbiology, 65 5193-7 (1999).
  • Schoch C.L., Seifert K.A., Huhndorf S., Robert V., Spouge J.L., Levesque C.A., Chen W., Consortium F.B. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi, Pnas, 109(16) 6241–6246 (2012).
  • Senses-Ergul Ş., Agoston R., Belak A., Deak T. Characterization of some yeasts isolated from foods by traditional and molecular tests, International Journal of Food Microbiology, 108(1) 120-124 (2006).
  • Shin K.S, Shin Y.K, Yoon J.H, Park Y.H. Candida thermophila sp. nov., a novel thermophilic yeast isolated from soil, International Journal of Systematic Evolutionary Microbiology, 51 2167–2170, (2001).
  • Šuranská H., Raspor P., Uroić K., Golić N., Kos B., Mihajlović S., Begović J., Šušković J., Topisirović L., Čadež N. Characterisation of the yeast and mould biota in traditional white pickled cheeses by culture-dependent and independent molecular techniques, Folia Microbiologica (Praha), 61(6) 455-463 (2016).
  • Tofalo R., Chaves-López C., DiFabio F., Schirone M., Felis G.E., Torriani S., Paparella A., Suzzi G. Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must, International Journal of Food Microbiology, 130(3) 179-187 (2009).
  • Van den Burg B. Extremophiles as a source for novel enzymes, Current Opinion Microbiology, 6(3), 213-218 (2003).
  • Van Der Vossen J.M.B.M., Rahaoui H., De Nijs M.W.C.M.,Hartog B.J. PCR methods for tracing and detection of yeasts in the food chain. In: Yeasts in Food, Boekhout T, Robert V (eds), BayerleinGmbH, Neusass, Germany, p. 123-137, (2003).
  • Vaughan-Martini A. Reflections on the clasification of yeasts for different end-users in biotechnology, ecology, and medicine, International Microbiology, 6(3) 175-182 (2003).
  • White T.J., Bruns T., Lee S., Taylor J.W. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A.,Gelfand, D.H., Snissky, J.J., White, T.J. (Eds.), PCR protocols: A guide to method sand applications. AcademicPressInc.,NewYork, p. 315–322, (1990).
  • Zhang Y., Wu W.P., Hu D.M., Su Y.Y., Cai L. A new thermophilic species of Myceliophthora from China, Mycological Progress, 13(1)165–170 (2014).

Ayrıntılar

Konular Fen
Bölüm MİKOLOJİ
Yazarlar

H. Halil BIYIK

Esin POYRAZOĞLU ÇOBAN

Yusuf GEROĞLU

İlgıt KIRGIZ

Yayımlanma Tarihi 28 Nisan 2017
Yayınlandığı Sayı Yıl 2017, Cilt 8, Sayı 1

Kaynak Göster

Bibtex @araştırma makalesi { mantar309628, journal = {Mantar Dergisi}, issn = {}, eissn = {2147-6845}, address = {}, publisher = {Selçuk Üniversitesi}, year = {2017}, volume = {8}, pages = {48 - 59}, doi = {10.15318/Fungus.2017127496}, title = {Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı}, key = {cite}, author = {Bıyık, H. Halil and Poyrazoğlu Çoban, Esin and Geroğlu, Yusuf and Kırgız, İlgıt} }
APA Bıyık, H. H. , Poyrazoğlu Çoban, E. , Geroğlu, Y. & Kırgız, İ. (2017). Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı . Mantar Dergisi , 8 (1) , 48-59 . Retrieved from https://dergipark.org.tr/tr/pub/mantar/issue/28930/309628
MLA Bıyık, H. H. , Poyrazoğlu Çoban, E. , Geroğlu, Y. , Kırgız, İ. "Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı" . Mantar Dergisi 8 (2017 ): 48-59 <https://dergipark.org.tr/tr/pub/mantar/issue/28930/309628>
Chicago Bıyık, H. H. , Poyrazoğlu Çoban, E. , Geroğlu, Y. , Kırgız, İ. "Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı". Mantar Dergisi 8 (2017 ): 48-59
RIS TY - JOUR T1 - Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı AU - H. Halil Bıyık , Esin Poyrazoğlu Çoban , Yusuf Geroğlu , İlgıt Kırgız Y1 - 2017 PY - 2017 N1 - DO - T2 - Mantar Dergisi JF - Journal JO - JOR SP - 48 EP - 59 VL - 8 IS - 1 SN - -2147-6845 M3 - UR - Y2 - 2017 ER -
EndNote %0 Mantar Dergisi Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı %A H. Halil Bıyık , Esin Poyrazoğlu Çoban , Yusuf Geroğlu , İlgıt Kırgız %T Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı %D 2017 %J Mantar Dergisi %P -2147-6845 %V 8 %N 1 %R %U
ISNAD Bıyık, H. Halil , Poyrazoğlu Çoban, Esin , Geroğlu, Yusuf , Kırgız, İlgıt . "Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı". Mantar Dergisi 8 / 1 (Nisan 2017): 48-59 .
AMA Bıyık H. H. , Poyrazoğlu Çoban E. , Geroğlu Y. , Kırgız İ. Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı. Değerlendirme süreci. 2017; 8(1): 48-59.
Vancouver Bıyık H. H. , Poyrazoğlu Çoban E. , Geroğlu Y. , Kırgız İ. Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı. Mantar Dergisi. 2017; 8(1): 48-59.
IEEE H. H. Bıyık , E. Poyrazoğlu Çoban , Y. Geroğlu ve İ. Kırgız , "Aydın İlinde Termofilik Çevreden İzole Edilen Mayaların Moleküler Tanısı", Mantar Dergisi, c. 8, sayı. 1, ss. 48-59, Nis. 2017, doi:10.15318/Fungus.2017127496
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