Antioxidant, antimicrobial activities and total flavonoid contents of Cirsium bulgaricum DC. leaf extracts
Abstract
In this study, the antioxidant activities of methanol and
acetone extracts of Cirsium bulgaricum DC. (Asteraceae)
were evaluated by five antioxidant assays, including
phosphomolybdate method, cupric ion reducing capacity
(CUPRAC), 2,2’-azinobis(3-ethylbenzothiazoline-6-
sulfonicacid)/persulfate) ABTS/Persulfate, N, N-dimethyl-pphenylenediamine
(DMPD) and a Ce(IV)-based reducing
capacity (CERAC) methods. The 80% methanol and acetone
extracts showed 0.005-6.16±0.03 mmol/g and 0.004-
5.02±0.02 [(trolox (TR), catechin (CT), epicatechin (EC),
rutin (RT), quercetin (QR), naringin (NG) in CUPRAC and
ABTS/Persulfate methods, respectively]. According to DMPD
methods, inhibition effect were showed 2.3%±0.02 for 80%
methanol and 4.6%±0.07 for acetone. Ascorbic acid equivalent
mmol (AA)/g extract in CERAC method was found to be
0.0834±0.09 for 80% methanol and 0.5622±0.05 for acetone.
Ascorbic acid equivalent μg ascorbic acid (AA) /mL extract
were found 807.6±0.07 for 80% methanol and 292.4±0.01 for
acetone in phpsphomolybdate method. In the addition to the
antioxidant activity of the extract, the total flavonoid content
was measured. Total flavonoid content of 80% methanol and
acetone extracts was found to be 105.5-645±0.04 μg/ml
quercetin and rutin equivalent. For the determination of
antibacterial activities Escherichia coli (E.coli) ATCC 25922,
Escherichia coli(E.coli) O157:H7 ATCC 33150, Salmonella
E n t e r i t i d i s(S.E n t e r i t i d i s ) AT C C 1 3 0 7 6 , Listeria
monocytogenes(L.monocytogenes) AT C C 7 6 4 4 ,
Staphylococcus aureus(S.aureus) ATCC 25923 bacteria were
used as test bacteria. Acetone extracts showed antibacterial
activity against all of the tested bacteria ranged between
17.00±1.72- 19.88±1.75 mm and 80% methanol extracts
showed antibacterial activity against all of the tested bacteria
ranged between 11.35±1.43-14.43±1.32 mm.
Keywords: Antioxidant Activity, Antimicrobial Activity,
Total Flavonoid Contents, Cirsium bulgaricum
References
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Abstract
References
- Larson RA. The antioxidants of higher plants. Photochem 1988; 4 : 969–978.
- Hertog MGL, Hollman PCH, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Nederlands. J Agric Food Chem 1992; 40 : 2379–83.
- Cao G, Sofic E, Prior RL. Antioxidant capacity of tea and common vegetables. J Agric Food Chem 1996; 44 :3426– 3431.
- Kivits GAA, Vam der Sman FJP, Tijburg LBM. Analysis of catechin from green and black tea in humans: a specific and sensitive colorimetric assay of total catechins in biological fluids. Int J Food Sci Nutr 1997; 48 : 387–92.
- Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharmacol 2000; 32 :81–118.
- Exarchou V, Nenadis N, Tsimidou M, Gerothanassis IP, Troganis A, Boskou D. Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage and summer savory. J Agric Food Chem 2002; 50 : 5294–5299.
- Hirasa K, Takemasa M. Spice science and technology. New York: Marcel Dekker, 1998.
- Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998; 46 : 4113–7.
- Chen YT, Li XJ, Zao BL, Zheng RL, Xin WJ. ESR study of the scavenging effect of natural products of rutin etc superoxide and hydroxyl radical. ABBS 1989; 5 : 235–40.
- Dan N, Li XJ, Zhao BL, Zhang TM, Xin WJ. Scavenging effects of probimane on active oxygen free radicals by electron spin resonance. Acta Pharmacol Sin 1989; 10 :443–7
- Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971; 44 : 276–87.
- Luo QQ, Sen MC, Gao W, Peng Q. A study on reaction kinetics of superoxide dimutase and its model compounds with superoxide ion by illumination. Chem J Chin Univ 1990; 11 : 928–32.
- Gülçin I, Küfrevioğlu İ, Oktay M, Büyükokuroğlu ME. Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica diocia L.). J Ethnopharmacol 2004; 90: 205–15.
- Qu B, Huang D, Hampsch-Woodill M, Flanagan JA, Deemer EK. Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | January 9, 2015 |
| Published in Issue | Year 2015 Volume: 19 Issue: 1 |