Antalya’da yetiştirilen holstein sığırlarında kompleks vertebral malformasyon kalıtsal hastalığının allele özgü PCR ile belirlenmesi

Year 2019, Volume: 32 Issue: 3, 443 - 446, 01.12.2019

Murat Gökçe Eren Murat Soner Balcıoğlu Eymen Demir

https://doi.org/10.29136/mediterranean.578015

Cited By: 2

Abstract

Bu çalışmada kalıtsal bir hastalık olan kompleks vertebral
malformasyonun (CVM) Antalya ilinde yetiştiriciliği yapılan Siyah Alaca
sığırlarında varlığı allele özgü polimeraz zincir reaksiyonu (AS-PCR)
kullanılarak araştırılmıştır. Antalya Damızlık Sığır Yetiştiricileri Birliğine
üye işletmelerden ve aynı birliğe üye olan Akdeniz Üniversitesi, Ziraat
Fakültesi süt sığırcılığı işletmesine ait toplam 200 bireyde CVM kalıtsal
hastalığının varlığı araştırılmıştır. Holstein sığırlarından elde edilen kan
örneklerinden total genomik DNA izole
edilmiştir. Sığır genomunun 3. kromozomu üzerinde
bulunan SLC35A3 geninin 395 bç
uzunluğundaki bölgesinde normal ve mutant allellerin belirlenmesinde farklı
primerler kullanılmıştır. AS-PCR işleminden sonra uygulanan agaroz jel
elektroforezi sonuçlarına göre bireyler genotiplendirilmiştir. Bu çalışmada;
incelenen 200 bireyden 7 tanesinin CVM taşıyıcısı olduğu belirlenmiştir.

Keywords

CVM, SLC35A3 geni, Kalıtsal hastalıklar

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2014.02.0121.016

Thanks

Bu çalışma Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 2014.02.0121.016 nolu proje ile desteklenmiştir.

Detection of complex vertebral malformation in holstein cattle reared in Antalya using allele-spesific PCR

Abstract

This
study aimed to analyze complex vertebral malformation (CVM) genetic defect in
Holstein raised in Antalya province using allele specific PCR (AS-PCR). The
presence of CVM mutant allele was investigated in 200 Holstein cows raised by
member breeders of Antalya Cattle Breeders Association and raised in Akdeniz
University, Faculty of Agriculture. Total genomic
DNA was extracted from blood samples obtained from Holstein cows. In the 395 bp
length of SLC35A3 gene region of
bovine chromosome 3, different primers were used to
identify the normal and mutant allele. By using agarose gel electrophoresis,
individuals were genotyped after AS-PCR. In this study, 7 of 200 individuals
were found as CVM carriers.

Keywords

CVM, SLC35A3 gene, Hereditary diseases

Project Number

2014.02.0121.016

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