Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations

Year 2020, Volume: 33 Issue: 1, 145 - 148, 01.04.2020

Eymen Demir , Bahar Argun Karslı , Taki Karslı , Murat Soner Balcıoğlu

https://doi.org/10.29136/mediterranean.633476

Abstract

Nerve Growth Factor Gene (NGF)
has important functions in the maintenance of viability and proliferation of
ovarian cells. Recent studies on caprine NGF gene revealed two SNPs
(determined by SacII/C291A and MboII/A705G
polymorphisms) related with body length and litter
size in goats. This study aimed to
determine SacII and MboII polymorphisms of NGF in native
Turkish goat populations including Turkish Hair (HAI), Kabakulak (KBK), Honamlı
(HNM) and Norduz (NRD) by using PCR-RFLP. Amplificated PCR fragments of 808
were digested with SacII,
while amplificated PCR fragments of 319 bp were digested with MboII restriction enzyme in order to
detect different genotypes of NGF gene. The “A” allele frequency ranged
from 0.500 (HAI, HNM and KBK) to 0.950 (NRD), while the “C” allele frequency
ranged from 0.025 (NRD) to 0.500 (HAI, HNM and KBK) in NGF/SacII
polymorphism. The “A” allele frequency ranged from 0.213 (HAI) to 1.000
(NRD), while the “G” allele frequency ranged from 0.000 (NRD) to 0.787 (HAI) in
NGF/MboII
polymorphism. Deviation from HW equilibrium was significant in HNM goat
population (P < 0.05). In this
study, polymorphisms of caprine NGF gene in native Turkish goat
populations were revealed for the first time. The
results obtained from this study showed that NGF/SacII polymorphisms could be used for
body length in NRD population while NGF/MboII polymorphisms could be used for litter size in HAI, HNM and
KBK populations in MAS studies.

Keywords

Body length, Litter size, Nerve Growth Factor, NGF, PCR-RFLP

Türkiye’de yetiştirilen dört keçi populasyonunda Sinir Büyüme Faktörü (NGF) geninde SacII ve MboII polimorfizmlerinin belirlenmesi

Abstract

Sinir
Büyüme Faktörü (NGF) geni yumurtalık hücrelerinin yaşamını devam
ettirmesi ve çoğalmasında önemli fonksiyonlara sahiptir. Keçi NGF geni üzerinde
yapılan son çalışmalar, iki SNP’in (SacII/C291A ve MboII/A705G
polimorfizmi) vücut uzunluğu ve bir batında doğan yavru sayısı ile ilişkili
olduğunu ortaya çıkarmıştır. Bu çalışmada, Türkiye’de yetiştirilen Kıl (HAI),
Kabakulak (KBK), Honamlı (HNM) ve Norduz (NRD) keçi populasyonlarının NGF
geninde SacII ve MboII polimorfizminin PCR-RFLP yöntemiyle
belirlenmesi amaçlanmıştır. NGF geninde farklı genotiplerin belirlenmesi
için çoğaltılan 808 bç uzunluğundaki PCR ürünleri SacII, 319 bç
uzunluğundaki PCR ürünleri ise MboII restriksiyon enzimi ile
kesilmiştir. NGF/SacII polimorfizminde A allel frekansı 0.500
(Kıl, Honamlı ve Kabakulak) ile 0.950 (Norduz) aralığında değişirken, C allel
frekansı 0.025 (Norduz) ile 0.500 (Kıl, Honamlı ve Kabakulak) aralığında
değişmiştir. NGF/MboII polimorfizminde A allel frekansı 0.213
(Kıl) ile 1.000 (Norduz) aralığında değişirken, G allel frekansı 0.000 (Norduz)
ile 0.787 (Kıl) aralığında değişmiştir. Honamlı populasyonunda HW dengesinden
sapma önemli bulunmuştur (P < 0.05).
Bu çalışmada, Türkiye’nin yerli keçi populasyonlarında keçi NGF gen
polimorfizmi ilk defa ortaya çıkarılmıştır. Bu
çalışmandan elde edilen sonuçlar, NGF/SacII polimorfizminin Norduz popuslayonunda vücut uzunluğu, için, NGF/MboII polimorfizminin ise Kıl, Honamlı
ve Kabakulak populasyonlarında bir batında doğan yavru sayısı için markör
destekli seleksiyon (MAS) çalışmalarında kullanılabileceğini göstermiştir.

Keywords

Vücut uzunluğu, Yavru sayısı, Sinir Büyüme Faktörü, NGF, PCR-RFLP

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