Research Article

Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss)

Volume: 12 Number: 2026 October 24, 2026
EN TR

Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss)

Abstract

The aim of this research was to identify the number of alpha-naphthyl acetate esterase (ANAE) positive T lymphocytes in the peripheral blood of rainbow trout (Oncorhynchus mykiss). Peripheral blood was collected from 10 healthy rainbow trout obtained from a trout farm in Iğdır province. ANAE histochemical identification was performed on the prepared blood smear slides, and the slides were observed under a light microscope. ANAE-positive lymphocytes, which have brown-red reaction granules in their cytoplasm, were identified as T lymphocytes. ANAE positive cell rates were determined after counting 200 lymphocytes from each slide. As a result of this research, there was no positive ANAE reaction in basophilic, eosinophilic, and pseudo-eosinophilic cells, and a negative or weak reaction was identified in monocytes. While there was considerable ANAE positivity in some lymphocytes, there was no reaction in others. The ANAE-positive T lymphocytes rate in rainbow trout was found to be 61.45 ± 2.13 %. It can be concluded that ANAE determination is a reliable, economical, and applicable histochemical method for T lymphocyte detection in rainbow trout, and that it can give useful information regarding the immune system in fish, disease immunologic monitoring, and efficiency of vaccination studies.

Keywords

Supporting Institution

NO

Project Number

No financial support was received for the present study.

Ethical Statement

Permission to carry out this experiment was taken from the Animal Experiments Ethics Committee of Kafkas University under the code KAU-HADYEK/2026-112.

Thanks

NO

References

  1. Aştı, R. N. (1996). Farklı hayvan türlerinin perifer kan lökositlerinde alfa naftil asetat esteraz aktivitesinin belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 43(02), 129-133. https://doi.org/10.1501/Vetfak_0000000704
  2. Bayraktaroğlu, A. G., Şimşek, Ö., Kürüm, A., Arıkan, Ş., & Ergün, E. (2015). Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats. Turkish Journal of Veterinary & Animal Sciences, 39(1), 57-61. DOI: 10.3906/vet-1407-44
  3. Blaxhall, P. C., & Hood, K. (1985). Cytochemical enzyme staining of fish lymphocytes separated on a Percoll gradient. Journal of Fish Biology, 27(6), 749-755. https://doi.org/10.1111/j.1095-8649.1985.tb03217.x
  4. Çelik İ, Aştı, R. N., & Boyraz, M. Ü. (1992). Light microscopic studies on the alpha-naphthyl acetate esterase activity of the fetal bovine peripheral blood lymphocytes. Eurasian Journal of Veterinary Sciences, 8(2), 41-44.
  5. Dönmez, H. H., & Sur, E. (2007). Determination of the alpha-naphthyl acetate esterase (ANAE) and acid phosphatase (ACP) activity in the peripheral blood lymphocytes of the adult duck (Anas platryhynchos). Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2 (4), 122-128.
  6. Dönmez, H. H., Sur, E., & Boydak, M. (2007). Determination of alpha naphthyl acetate esterase activity in peripheral blood leucocytes of Kangal fish (Garra rufa). Eurasian Journal of Veterinary Sciences, 23(2), 81-84.
  7. Kadıralieva, N., Sur, E., Öznurlu, Y., & Özaydın, T. (2020). Kırgızistan dağ yaklarının (Bos grunniens) perifer kan lökositlerinde alfa naftil asetat esteraz (ANAE) pozitivitesinin belirlenmesi. Eurasian Journal of Veterinary Sciences, 36(3) https://doi.org/10.15312/EurasianJVetSci.2020.276
  8. Kılıçoğlu, B., Uyarlar, C., Tunç, A. C., Başer, D. F., Yipel, F. A., Birdane, F. M. & Acar, A. (2017). Taylara oral olarak uygulanan bitkisel yağ ekstraktı karışımının (nane, kekik, anason) immun sistem üzerine etkisi. Kocatepe Veterinary Journal, 10(4), 287-294. DOI: 10.5578/kvj.63933

Details

Primary Language

English

Subjects

Fish Biology

Journal Section

Research Article

Publication Date

October 24, 2026

Submission Date

July 12, 2026

Acceptance Date

September 30, 2026

Published in Issue

Year 2026 Volume: 12 Number: 2026

APA
Akgün, A. (2026). Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss). MEMBA Su Bilimleri Dergisi, 12(2026). https://doi.org/10.58626/memba.1993069
AMA
1.Akgün A. Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss). MEMBA Su Bilimleri Dergisi. 2026;12(2026). doi:10.58626/memba.1993069
Chicago
Akgün, Ayhan. 2026. “Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss)”. MEMBA Su Bilimleri Dergisi 12 (2026). https://doi.org/10.58626/memba.1993069.
EndNote
Akgün A (October 24, 2026) Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss). MEMBA Su Bilimleri Dergisi 12 2026
IEEE
[1]A. Akgün, “Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss)”, MEMBA Su Bilimleri Dergisi, vol. 12, no. 2026, Oct. 2026, doi: 10.58626/memba.1993069.
ISNAD
Akgün, Ayhan. “Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss)”. MEMBA Su Bilimleri Dergisi 12/2026 (October 24, 2026). https://doi.org/10.58626/memba.1993069.
JAMA
1.Akgün A. Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss). MEMBA Su Bilimleri Dergisi. 2026;12. doi:10.58626/memba.1993069.
MLA
Akgün, Ayhan. “Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss)”. MEMBA Su Bilimleri Dergisi, vol. 12, no. 2026, Oct. 2026, doi:10.58626/memba.1993069.
Vancouver
1.Ayhan Akgün. Determination And Evaluation Of Anae Positive T Lymphocytes In Raınbow Trout (Oncorhynchus Mykiss). MEMBA Su Bilimleri Dergisi. 2026 Oct. 24;12(2026). doi:10.58626/memba.1993069

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