Yetiştiriciliği Yapılan Mersin Balıklarından İzole Edilen Bakterilerin Antimikrobiyal Direnç Profillerinin Belirlenmesi

Year 2018, Volume: 4 Issue: 2, 7 - 13, 28.12.2018

Ertuğrul Terzi

Abstract

Türkiye'de mersin balıkları
yetiştiriciliği son yıllarda artmaya başlamıştır. Balıklar yetiştiricilik
aşamasında çeşitli hastalıklara maruz kalır ve bu hastalıklar ekonomik
kayıplara sebep olabilmektedir. Bu çalışmada karaca (Acipenser
gueldenstaedtii) ve sibirya (Acipenser baerii) mersin balıklarından
ortalama ağırlıkları 3 kg’a ulaşıncaya kadar geçen sürede izole edilen toplam
37 suşun antimikrobiyal maddelere karşı direnç profilleri ve direnç genlerin
varlığı araştırılmıştır. Bakterilerin en fazla dirençli oldukları
antibiyotikler sulfametoksazol ve ampisilin olarak tespit edilmiştir (%97.3).
Bunun yanında bakterilerin test edilen antibiyotikler arasında gentamisin,
oksolinik asit, trimetoprim+sulfametoksazol ve florfenikole karşı
hassasiyetleri sırasıyla %86.5, %59.5, %45.9 ve %40.5 olarak tespit edilmiştir.
Bakterilerde taranan genlerden tetA ve tetB genleri sırasıyla %
10.8 ve 21.6 olarak tespit edilmiştir. En fazla tespit edilen genler % 24
oranla ampC ve cmlA genleri olmuştur. Bu genleri % 19’luk oranla sul3 ve floR genleri
takip etmiştir. Gerçekleştirilen bu çalışmada,
bakterilerin antibiyotiklere karşı direnç seviyelerinin oksitetrasiklin,
streptomisin, enrofloksasin ve ampisilinde %50’den yüksek olması, mersin balığı
yetiştiriciliğinde bakteriyel hastalıkların tedavisinde gelecekte sorunlar
yaşanacağını söylenebilir.

Keywords

Antimikrobiyal, Antibiyogram, Direnç genleri, Mersin Balığı

Determination of Antimicrobial Resistance Profiles of the Bacteria Isolated from Cultured Sturgeons

Abstract

Sturgeon farming in Turkey has increased in recent years. Fish are
exposed to various diseases during the culturing period and these diseases can
cause economic losses. In this study, a total of 37 strains isolated from
diamond (Acipenser gueldenstaedtii) and siberian (Acipenser baerii)
sturgeons till their average weights reach up to 3 kg were used to determine
resistance profiles against antimicrobial substances and investigated their
resistance genes. The most resistance to antibiotics in bacteria were found as
sulphamethoxazole and ampicillin (97.3%). Moreover, the susceptibility of
bacteria to the tested antibiotics for gentamicin, oxolinic acid, trimethoprim
+ sulfamethoxazole and florfenicol was determined as 86.5%, 59.5%, 45.9% and
40.5%, respectively. tetA ve tetB genes in the tested bacteria were detected as 10.8% and 21.6%,
respectively. The most common genes were ampC and cmlA
genes (24%) followed by sul3 and floR genes with a ratio of 19%.
In this study, it can be said that there may be problems in the treatment of
bacterial diseases in sturgeon farming because of being higher than 50% of the
resistance levels of bacteria against antibiotics such as oxytetracycline,
streptomycin, enrofloxacin and ampicillin.

Keywords

Antimicrobial, Antibiogram, Resistance Genes

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