AbstractInvestigation of Cryptosporidium Parvum by ELISA and Modified Ehrlich-Ziehl-Neelsen Stain Methods in Patients With Diarrhea Objective: Cryptosporidium spp are important agents causing diarrhea in developing and developed countries. The agent causes severe life-threatening diarrhea especially in immunocompromised hosts. Furthermore, transmission of Cryptosporidium parvum by of drinking water threats public health. The aim of this study was to compare the two methods used in determining Cryptosporidium parvum. Method: In this study 92 diarrheic stool specimens from inhabitants of Malatya Rest Home and patients with diarrhea applied to Inonu University Turgut Ozal Medical Center in Malatya are investigated for Cryptosporidium parvum copro-antigens by Enzyme-Linked Immunosorbent Assay (ELISA) and oocysts via modified Ehrlich-Ziehl-Neelsen (m-EZN) staining and both methods are compared statistically. Results: Five specimens are found to be positive by both ELISA and m-EZN. The sensivity and spesifity of ELISA was 100% in this study. Conclusion: m-EZN staining method can be used especially in immuncompromised patients with diarrhea as it is a rapid, cost-effective and easily applicable method for routinely diagnosis of Cryptosporidium parvum.
Türkçe Özet:Amaç: Cryptosporidium türleri gelişmekte olan ve gelişmiş ülkelerde ishale neden olan parazitler arasında önemli bir yere sahiptir. Özellikle Cryptosporidium parvum, immün sistemi baskılanmış kişilerde ölümcül ishallere neden olabilmektedir ve ayrıca içme sularıyla taşınabildiği için halk sağlığını tehdit edebilmektedir. Bu çalışmada Cryptosporidium parvum\'un saptanması için kullanılan iki yöntemin karşılaştırılması amaçlandı. Yöntem: Bu çalışmada, Malatya Huzurevi sakinleri ile İnönü Üniversitesi Turgut Özal Tıp Merkezi\'ne başvuran ishalli toplam 92 kişiden alınan ishalli dışkı örneklerinde, Enzyme-Linked Immunosorbent Assay (ELISA) yöntemiyle Cryptosporidium parvum koproantijenleri ve modifiye Ehrlich-Ziehl-Neelsen (m-EZN) boyama yöntemiyle ookistleri araştırılmış; iki yöntem istatistiksel olarak karşılaştırılmıştır. Bulgular: Toplam beş örnekte hem ELISA ile hem de m-EZN yöntemiyle pozitiflik saptanmıştır. ELISA yönteminin duyarlılığı ve özgüllüğü bu çalışmada %100 olarak saptanmıştır. Sonuç: Özellikle immün yetmezlikli ishalli hastalarda Cryptosporidium parvum tanısında hızlı, ucuz, kolay uygulanabilen bir yöntem olan m-EZN yönteminin rutin uygulamalarda kullanılabileceği düşünülmektedir.
Primary Language | Turkish |
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Journal Section | Articles |
Authors | |
Publication Date | March 1, 2008 |
Submission Date | June 13, 2014 |
Published in Issue | Year 2008 Volume: 1 Issue: 1 |
MEU Journal of Health Sciences Assoc was began to the publishing process in 2008 under the supervision of Assoc. Prof. Gönül Aslan, Editor-in-Chief, and affiliated to Mersin University Institute of Health Sciences. In March 2015, Prof. Dr. Caferi Tayyar Şaşmaz undertook the Editor-in Chief position and since then he has been in charge.
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