Identification and Prevalence of Ralstonia Solanacearum from Potato Fields of Kyrgyzstan

Year 2014, Volume: 4 Issue: 1, 1 - 9, 01.06.2014

Tinatin Döölotkeldieva Saykal Bobuşeva

Abstract

For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed during storage. For ELISA was used Ralstonia solanacerium PathoScreen R Kit DAS ELISA (Agdia product, USA).Isolated races of Ralstonia solanacearum by biochemical characteristics were classified as a 3-biotype.

Keywords

the potato (Solanum tuberosum) cultivars, identification of Ralstonia solanacearum , ELISA assay, biochemical tests

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