Kompostlardan elde edilen antagonist bakteri izolatlarının kavun solgunluk hastalığı etmeni Fusarium oxysporum f.sp. melonis’e karşı in vitro antagonistik etkilerinin belirlenmesi

Year 2022, , 565 - 577, 01.12.2022

Senem Özkaya , Emine Mine Soylu

https://doi.org/10.37908/mkutbd.1139502

Cited By: 1

Abstract

Amaç: Bu çalışmada zeytin, pamuk ve hayvansal atıklardan yapılmış 5 farklı komposttan antagonistik etkinliğe sahip bakterilerin izolasyonu, tanılanması ve kavun solgunluk hastalığı etmeni Fusarium oxysporum f. sp. melonis (Fom)’in misel gelişimini engellemeleri üzerine olan etkinlikleri in vitro koşullarda araştırılmıştır.

Yöntem ve Bulgular: Kompostların fermentasyon süresine bağlı olarak bakteriyel popülasyon sayısında artış görülmüştür. En yüksek bakteri popülasyonu fermentasyonun 3 ve 4. aylarında belirlenmiştir. Farklı kompost materyallerinden izole edilen antagonist adayı bakteri izolatları Yağ Asit-Metil Ester (FAME) profillerine göre Mikrobiyal Tanılama Sistemi (MIS) ile teşhis edilmiştir. Aday bakterilerin Fom’in misel gelişimini engelleme potansiyelleri ikili kültür testleri ile belirlenmiştir. Elde edilen aday bakteriyel izolatlar arasında 31 bakteri izolatı ikili kültür denemelerinde, Fom’in misel gelişimini engellenmesi üzerine değişik oranlarda antagonistik etkinlik göstermiştir. Antagonistik potansiyele sahip bakteri izolatlarının çoğunluğunu farklı Bacillus spp.’a ait izolatlar (% 73.3) oluşturmuştur. Farklı kompost materyallerinden izole edilen bakteriler arasında en yüksek antagonistik etki %48.33 engelleme oranı ile Entereobacter gergoviae K4B:4:7:1 izolatı tarafından gösterilmiş olup, bu izolatı sırasıyla Bacillus cereus K1B:4:8:1 (%47.5), Salmonella typhimurium K5B:1:4:3 (% 46.67), Bacillus amyloliquefaciens K5B:0:5:1 (%43.33) ve Bacillus subtilis K3B:4:8:1 (%40.83) izolatları izlemiştir.

Genel Yorum: Organik atıklardan hazırlanmış kompostlar toprak kökenli hastalıklara karşı etkin antagonistik etkiye sahip bakterilere konukçuluk etmektedir. Kompostların in vivo koşullarda Fom’e karşı kullanılma potansiyellerinin araştırılmasının yanı sıra buralardan biyolojik preparat olarak kullanıma en uygun olan bakteri izolatların seçimi ve hastalığı engellemede kullandıkları mekanizmaların belirlenmesi üzerine çalışmalar gelecekte sürdürülmelidir.

Çalışmanın Önemi ve Etkisi: Elde edilen sonuçlar, Fom’in biyolojik mücadelesinde antagonistik potansiyele sahip mikroorganizmaların yoğun olarak geliştiği kompostların seralar gibi sınırlı alana sahip yetiştirme alanlarında kullanılmasının etkin bir rol oynayabileceğini göstermiştir. 

Keywords

Fusarium oxysporum f. sp. melonis, kavun, kompost, antagonist, biyolojik mücadele

Supporting Institution

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı

Project Number

MKU BAP 1101Y0110

Thanks

Bu çalışma Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı tarafından finansal olarak desteklenmiştir (Proje Numarası: MKU BAP 1101Y0110).

Determination of antagonistic effects antagonist bacterial isolates obtained from composts against melon wilt disease agent Fusarium oxysporum f.sp. melonis in vitro conditions

Abstract

Aims: Isolation, identification and antagonistic potentials of bacteria obtained from 5 different composts, which were made from olive cake, cotton seed cake and animal manure, were investigated on inhibition of mycelial growth of the melon wilt disease agent Fusarium oxysporum f. sp. melonis (Fom) in vitro conditions.

Methods and Results: An increase in the number of bacterial populations was observed depending on the fermentation of the composts. The highest bacterial population was determined in 3rd and 4th months of fermentation. The putative antagonist bacterial isolates obtained from different compost materials were identified by Microbial Identification System (MIS) according to their Fatty Acid-Methyl Ester (FAME) profiles. The antagonistic potentials of bacteria against Fom were determined by dual culture tests. Among the putative bacterial isolates obtained, 31 bacterial isolates showed antagonistic activity on inhibition of mycelial growth of Fom at varying rates in dual culture tests. Most of the bacterial isolates having antagonistic potential were identified as Bacillus spp. (73.3%). Among the antagonistic bacteria isolated from different compost materials, the highest antagonistic effect was displayed by Enterobacter gergoviae K4B:4:7:1 with a 48.33% inhibition rate, and this isolate was followed by Bacillus cereus K1B:4:8:1 (47.5%), Salmonella typhimurium K5B:1:4:3 (46.67%), Bacillus amyloliquefaciens K5B:0:5:1 (43.33%) and Bacillus subtilis K3B:4:8:1 (40.83%) isolates, respectively.

Conclusions: Composts prepared from organic wastes host effective bacteria that have an antagonistic effect against soil-borne diseases. Future studies should be conducted to investigate the in vivo potential of composts against Fom, to select the most suitable bacterial isolates for use as biological preparations, and to identify mechanisms used to prevent disease development.

Significance and Impact of the Study: The results indicated that the use of organic waste composts, where microorganisms with antagonistic potential are intensively developed, can play an effective role in growing areas with limited space such as greenhouses.

Keywords

Fusarium oxysporum f. sp. melonis, melon, compost, antagonist, biological control

Project Number

MKU BAP 1101Y0110

References

• Alimi M, Soleimani MJ, Darzi MT (2012) Characterization and application of microbial antagonists for control of Fusarium head blight of wheat caused by Fusarium graminearum using single and mixture strain of antagonistic bacteria on resistance and susceptible cultivars. African J. Microbiol. Res. 6: 326-334.
• Angelopoulou DJ, Naska EJ, Paplomatas EJ, Tjamos SE (2014) Biological control agents (BCAs) of V. dahliae wilt: influence of application rates and delivery method on plant protection, triggering of host defense mechanisms and rhizosphere populations of BCAs. Plant Pathol. 63: 1062–1069.
• Anonymous (2020) Food and Agriculture Organization of the United Nations. https://www.fao.org Erişim tarihi 10.03.2022.
• Avilés M, Borrero C, Trillas MI (2011) Review on compost as an inducer of disease suppression in plants grown in soilless culture. Dyn. Soil Dyn. Plant 5 (2): 1–11.
• Bonanomi G, Antignani V, Pane C, Scala F (2007) Suppression of soil borne fungal diseases with organic amendments. J. Plant Pathol. 89: 311–324.
• Bora T, Ozaktan H, Göre E, Aslan E (2004) Biological control of Fusarium oxysporum f. sp. melonis by Wettable Powder formulations of the two strains of Pseudomonas putida. J. Phytopathol. 152: 471-475.
• Bozkurt İA, Soylu S (2019) Elma kök uru hastalığı etmeni Rhizobium radiobacter’e karşı epifit ve endofit bakteri izolatlarının antagonistik potansiyellerinin belirlenmesi. Tekirdağ Zir. Fak. Derg. 16: 348-361.
• Cavigelli MA, Thien SJ (2003) Phosphorus bioavailability following incorporation of green manure crops. Soil Sci. Soc. America J. 67: 1186-1194.
• Chakraborty N, Chakraborty N, Acharyya P, Acharya K (2021) Isolation, characterization and identification of novel broad spectrum bacterial antagonist(s) to control Fusarium wilt of eggplant. Physiol. Molecular Plant Pathol. 116: 101711.
• Cheuk W, Kwang VL, Copeman R, Joliffe P, Fraser S (2005) Disease suppression on greenhouse tomatoes using plant waste compost. J. Environ. Sci. Health Part B, 40: 449-461.
• Chung YR, Hoitink HAJ (1990) Interactions between thermophilic fungi and Trichoderma hamatum in suppression of Rhizoctonia damping-off in a bark compost-amended container medium. Phytopathology, 80 : 73-77. Conklin AE, Susan ME, Liebman M, Lambert ED, Gallandt R, William Halteman A (2002) Effects of red clover (Trifolium pratense) green manure and compost soil amendments on wild mustard (Brassica kaber) growth and incidence of disease. Plant Soil. 238: 245-256.
• Corato UD, Salimbeni R, Pretis AD, Patruno L, Avella N, Lacolla G, Cucci G (2018) Microbiota from ‘next-generation green compost’ improves suppressiveness of composted Municipal-Solid-Waste to soil-borne plant pathogens. Biological Control 124: 1–17.
• Cruz-Martin M, Leyva L, Acosta-Suarez M, Pichardo T, Bermudez-Caraballoso I, Alvarado- Capo Y (2021) Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1. Agronom. Mesoameric. 32: 466-478.
• Delisoy K, Altınok HH 2019. Kavunda Fusarium solgunluk hastalığına karşı bazı rizobakterilerin ve bitki aktivatörlerinin etkinliklerinin belirlenmesi. Anadolu Tar. Bil. Derg. 34: 135-145.
• Dukare AS, Prasanna R, Dubey SC, Nain L, Chaudhary V, Singh R, Saxena AK (2011) Evaluating novel microbe amended composts as biocontrol agents in tomato. Crop Protection, 30: 436-442.
• Duniway JM (2002) Status of chemical alternatives to methyl bromide for preplant fumigation of soil. Phytopathology, 92: 1337- 1343.
• Emmert EAB, Handelsman J (1999) Biocontrol of plant disease: a (Gram -) positive perspective. FEMS Microbiol. Lett. 171: 1-9.
• Erzurum K, Taner Y, Secer E, Yanmaz R, Maden S (1999) Occurrence of races of F. oxysporum f.sp. melonis causing wilt on melon in Central Anatolia. J. Turk. Phytopathol. 28(3): 87-97.
• Evcil F, Yalçın O (1977) Ege Bölgesinde kavunlarda görülen solgunluk etmeni fungusların tespiti üzerinde ön araştırmalar. Zirai Mücadele Araştırma Yıllığı, 11:78.
• Ferraz HGM, Resende RS, Silveira PR, Andrade CCL, Milagres EA, Oliveira JR, Rodrigues FD (2014) Rhizobacteria induces resistance against Fusarium wilt of tomato by increasing the activity of defense enzymes. Bragantia, 73: 274-283.
• Hadar Y, Gorodecki B (1991) Suppression of germination of sclerotia of Sclerotium rolfsii in compost. Soil Biol. Biochem. 23: 303-306. Hardy G, Sivasithamparam K (1991) Suppression of Phytophthora root rot by a composted cucalyptus bark mix. Aust. J. Bot. 39: 153-159.
• Hariprasad P, Divakara ST, Niranjana SR (2011) İsolation and characterization of chitinolytic rhizobacteria for the management of Fusarium wilt in tomato. Crop Protection, 30: 1606-1612.
• Hoitink HAJ, Boehm MJ (1999) Biocontrol within the context of soilmicrobial communities: a substrate-dependent phenomenon. Annu. Rev. Phytopathol. 37: 427–446.
• Hoitink HAJ, Grebus ME (1997) Composts and the control of plant diseases. In: Humic Substances in Soils, Peats and Waters: Health and Enviromental Aspects (Hayes MHB, Wilson WS (eds), Royal Society of Chemistry, Cambridge, UK, pp. 359-366.
• Hoitink HAJ, Krause MS, Han DY (2001) Spectrum and mechanisms of plant disease control with compost. In: Stofella, P.J,. Kahn, B.A., (eds), Compost utilization in horticultural cropping systems. Lewis Publishers, Boca Raton, Florida, p. 263.
• Kara M, Soylu S (2022) Isolation of endophytic bacterial isolates from healthy banana trees and determination of their in vitro antagonistic activities against crown rot disease agent Fusarium verticillioides. MKU. Tar. Bil. Derg. 27(1): 36-46.
• Kefalogianni I, Gkizi D, Pappa E, Dulaj L, Tjamos SE, Chatzipavlidis I (2017) Combined use of biocontrol agents and zeolite as a management strategy against F. oxysporum f. sp. melonis and V. dahliae wilt. Biocontrol, 62: 139–150.
• Khalil MMR, Fierro-Coronado RA, Penuelas-Rubio O, Villa-Lerma AG, Plascencia- Jatomea R, Felix-Gastelum R, Maldonado-Mendoza IE, (2021) Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato. Saudi J. Biol. Sci. 28: 7460-7471.
• Kurt S, Baran B, Sarı N, Yetişir H (2002) Physiologic races of Fusarium oxysporum f.sp. melonis in the Southeastern Anatolia Region of Turkey and varietal reactions to races of the pathogen. Phytoparasitica, 30(4): 395-402.
• Larkin RP, Hopkins DL, Martin FN (1996) Suppression of Fusarium oxysporum and other microorganisms recovered from a disease-suppressive soil. Phytopathology, 86: 812-819.
• Lelliott RA, Stead DE (1987) Methods for the diagnosis of bacterial disease of plants. In: Methods in Plant Pathology, (Preece TF, Ed.), Blackwell Scientific Publications, Oxford. pp 176-177,
• Li LL, Cheng H, Hua J, Yuan HH, Chen XL, Cheng SY (2015) Biological control of postharvest diseases of chestnut fruit by microbial antagonists-endophytic bacteria CE3. J. Pure Appl. Microbiol. 9: 57-67.
• Litterick AM, Harrier L, Wallace P, Watson CA, Wood M (2004) The role of uncomposted materials, composts, manures, and compost extracts in reducing pest and disease incidence and severity in sustainable temperate agricultural and horticultural crop production- a review. Crit. Rev. Plant Sci. 23: 453-479.
• Magid J, Kjaergaard C (2001) Recovering decomposing plant residues from the particulate soil organic matter fraction: size versus sepdensity separation. Biol. Fertil. Soils 33: 252-257.
• McKellar ME, Nelson EB (2003) Compost-induced suppression of Pythium damping-off is mediated by fatty-acid-metabolizing seed-colonizing microbial communities. Appl. Environ. Microbiol. 69: 452–460.
• Mendes R, Garbeva P, Raaijmakers JM (2013) The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms. FEMS Microbiol. Rev. 37: 634–663.
• Morales-Ruiz E, Priego-Rivera R, Figueroa-Lopez AM, Cazares-Alvarez JE, Maldonado-Mendoza IE (2021) Biochemical characterization of two chitinases from Bacillus cereus sensu lato B25 with antifungal activity against Fusarium verticillioides P03. FEMS Microbiol. Lett. 368: fnaa218.
• Noble R, Coventry E (2005) Suppression of soil-borne plant diseases with composts: A Rewiew. Biol. Sci. Technol. 15: 3-20.
• Ntougias S, Papadopoulou KK, Zervakis GI, Kavroulakis N, Ehaliotis C (2008) Suppression of soil-borne pathogens of tomato by composts derived from agroindustrial wastes abundant in Mediterranean regions. Biol. Fertil. Soils. 44, 1081–1090.
• Oztopuz O, Pekin G, Park RD, Eltem, R (2018) Isolation and evaluation of new antagonist Bacillus strains for the control of pathogenic and mycotoxigenic fungi of fig orchards. Appl. Biochem. Biotechnol. 186: 692-711.
• Pane C, Spaccini R, Piccolo A, Scala F, Bonanomi G (2011) Compost amendments enhance peat suppressiveness to Pythium ultimum, Rhizoctonia solani and Sclerotinia minor. Biol. Control 56: 115–124.
• Pascual JA, Hernandez T, Garcia C, De Leij FAAM, Lynch JM (2000) Long-term suppression of Pythium ultimum in arid soils using fresh and composted municipal wastes. Biol. Fert. Soils 30: 478-484.
• Pugliese M, Liu BP, Gullino ML, Garibaldi A (2011) Microbial enrichment of compost with biological control agents to enhance suppressiveness to four soilborne diseases in greenhouse. J. Plant Dis. Prot. 118 (2): 45–50.
• Ramarathnam R, Bo S, Chen Y, Fernando WGD, Gao XW, de Kievit T (2007) Molecular and biochemical detection of fengycin- and bacillomycin D-producing Bacillus spp., antagonistic to fungal pathogens of canola and wheat. Canadian J. Microbiol. 53: 901-911.
• Raviv M (2005) Production of high-quality composts for horticultural purposes a mini-rewiew. Hort Technol. 15: 52-57.
• Raviv M, Reuveni R, Zaidman BZ (1998) Improved medium for organic transplant. Biol. Agric. Hort. 16: 53-64.
• Reuveni R, Raviv M, Krasnovsky A, Freiman L, Medina S, Bar A, Orion D (2002) Compost induces protection against Fusarium oxysporum in sweet basil. Crop Protect. 21: 583-587.
• Sadfi N, Cherif M, Hajlaoui MR, Boudabbous A (2002) Biological control of the potato tubers dry rot caused by Fusarium roseum var. sambucinum under greenhouse, field and storage conditions using Bacillus spp. isolates. J. Phytopathol. 150: 640-648.
• San-Blas E, Carillo Z, Parra Y (2012) Effect of Xenorhabdus and Photorhabdus bacteria and their exudates on Moniliophthora roreri. Arch. Phytopathol. Plant Protect. 45: 1950-1967.
• Sasser, M. 1990. Identification of bacteria through fatty acid analyses. In: Methods in Phytobacteriology, Klement Z, Rudolph K, Sands DC (Eds.), Academiai Kiado, Budapest, Hungary, pp. 199– 204.
• Schreuder W, Lamprecht SC, Holz G (2000) Race determination and vegetative compatibility grouping of Fusarium oxysporum f.sp. melonis from South Africa. Plant Dis. 84:231-234.
• Singh N, Raina S, Singh D, Ghosh M, Heflish AIAI, (2017) Exploitation of promising native strains of bacillus subtilis with antagonistic properties against fungal pathogens and their PGPR characteristics. J. Plant Pathol. 99: 27-35.
• Soran H (1975) Ankara, Edirne, Sakarya illerinde kavun solgunluk hastalığı, fungal etmenlerinin tesbiti, dağılışları, bunlardan Fusarium türlerinin tanımı ve patojenisiteleri üzerinde araştırmalar (Doçentlik tezi) Ankara Üniversitesi, Ziraat Fakültesi (Basılmamış).
• Soylu EM, Soylu S, Kara M, Kurt Ş (2020) Sebzelerde sorun olan önemli bitki fungal hastalık etmenlerine karşı vermikomposttan izole edilen mikrobiyomların in vitro antagonistik etkilerinin belirlenmesi. KSU Tar. Doğa Derg. 23: 7-18.
• Soylu S, Kara M, Uysal A, Kurt Ş, Soylu EM (2021) Determination of antagonistic potential of endophytic bacteria isolated from lettuce against lettuce white mould disease caused by Sclerotinia sclerotiorum. Zemdirbyste-Agriculture, 108: 303-312.
• Suarez-Estrella F, Arcos-Nievas MA, Lopez MJ, Vargas-Garcia MC, Moreno J (2013) Biological control of plant pathogens by microorganisms isolated from agro-industrial composts. Biological Control 67: 509-515.
• Suarez-Estrella F, Elorrieta MA, Vargas-Garcia MC, Lopez MJ, Moreno J (2001) Selective isolation of antagonist micro-organisms of Fusarium oxysporum f. sp. melonis. Biological Control of Fungal and Bacterial plant Pathogens. IOBC Wprs Bullet. 24 (3): 109-112.
• Suarez-Estrella F, Vargas-Garcıa C, Lo´pez MJ, Capel C, Moreno J (2007) Antagonistic activity of bacteria and fungi from horticultural compost against Fusarium oxysporum f. sp. melonis. Crop Protect. 26: 46–53. Sülü SM, Bozkurt İA, Soylu S (2016) Bitki Büyüme Düzenleyici ve Biyolojik Mücadele Etmeni Olarak Bakteriyel Endofitler. MKÜ Zir. Fak. Derg. 21: 103-111.
• Tariq M, Khan A, Asif M, Khan F, Ansari T, Shariq M, Siddiqui MA 2020. Biological control: a sustainable and practical approach for plant disease management. Acta Agric Scand B Soil Plant Sci. 70: 507-524.
• Termorshuizen AJ, van Rijn E, van der Gaag DJ, Alabouvette C, Chen Y, Lagerlöf J, Malandrakis AA, Paplomatas EJ, Rämert B, Ryckeboer J, Steinberg C, Zmora-Nahum S (2006) Suppressiveness of 18 composts against 7 pathosystems: variability in pathogen response. Soil Biol. Biochem. 38: 2461–2477.
• Tuiter G, Szeech M, Bollen GJ (1998) Suppression of Rhizoctonia solani in potting mixtures amended with compost made from organic household waste. Phytopathology 88: 764-763.
• Weller DM, Raaaijmakers JM, McSpadden Gardener BB, Thomashow LS (2002) Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu. Rev. Phytopathol. 40, 309–348.
• Workneh F, Bruggen V, Drinkwater AHC, Sherman LEC (1993) Variables associated with a reduction in corky root and Phytophthora root rot of tomatoes in organic compared to conventional farms. Phytopathology 83: 581-589.
• Yazıcı Y, Aydın F, Tosun I, Kaklıkkaya N, Çaylan R, Köksal I (2004) Klinik örneklerden izole edilen Enterobacter suşlarının çeşitli antibiyotiklere direnç oranları. Türk. Mikrobiyal Cem. Derg. 34:29-32.
• Yıldız M (1977) Ege Bölgesinde kavun solgunluk etmeninin patojenisitesi, ırkları ve yerli çeşitlerin dayanıklılıklarının saptanması üzerinde araştırmalar (Doçentlik Tezi), E.Ü. Ziraat Fakültesi, Fitopatoloji ve Zirai Botanik Kürsüsü (Basılmamış).
• Yogev A, Raviv M, Hadar Y, Cohen R, Wolf S, Gil L, Katan J (2010) Induced resistance as a putative component of compost suppressiveness. Biological Control 54: 46-51.
• Yu X, Chengxiang A, Li X, Guangfang Z (2011) The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper. Eur. J. Soil Biol. 47: 138-145.
• Yücel S, Elekcioglu İH, Can C, Sogut MA, Ozarslandan A (2007) Alternative treatments to methyl bromide in Eastern Mediterranean Region of Turkey. Turk. J. Agric.For. 31: 47-53.
• Yücel S, Pala H, Sarı N, Abak K (1994) Determination of F. oxysporum f.sp. melonis races in the East Mediterranean Region of Türkiye and response of some melon genotypes to the disease. 9th Congress of the Mediterranean Phytopathological Union, Kuşadası-Türkiye. pp:87-89.
• Zhao Q, Dong C, Yang X, Mei X, Ran W, Shen Q, Xu Y (2011) Biocontrol of Fusarium wilt disease for Cucumis melo melon using bio-organic fertilizer. Appl. Soil Ecol. 47: 67-75.
• Zitter TA, Hopkins DL, Thomas CE (1996) Compendium of cucurbit diseases. APS Press, St. Paul.