Iris kerneriana (Çalı süseni) bitkisinin farklı kısımlarından elde edilen ekstraktların antioksidan özellikleri ve fenolik bileşenleri

Öz

Iris kerneriana Asch. & Sint. ex Baker bitkisinin farklı kısımlarının farklı çözücülerdeki antioksidan özellikleri ortaya koyulacak ve fenolik içeriği belirlenmiştir. Çalışma sonucunda bitki hakkında elde edilen bilgiler farklı sektörlerde kullanılmak üzere (gıda, kozmatik, ilaç vb) öncü olacaktır. Bitkinin içeriğinin ortaya konması; hem bitkinin içeriğinin aydınlatılması hem de insan sağlığına pozitif etkilerinin olduğu bilinen fenolik maddelerin varlığının tespiti, bitkinin tıbbi aromatik bir bitki olarak kullanımı için ön çalışma niteliğindedir. Araştırmada, I. kerneriana bitkisinin farklıkısımlarından elde edilen ekstraktların farklı çözücülerdeki fenolik bileşikleri tespit edildi ve antioksidan özellikleri analiz edildi. I. kerneira bitkisinin üç farklı kısmı üç farklı çözücü ile ekstre edildi. Bitki kısımlarına ve çözücülere göre dört farklı analiz yöntemine göre değerlendirildi. LC-MS/MS analizleriyle en yüksek düzeyde tespit edilen bileşiklerin, kateşin (187.87 mg/kg), klorojenik asit (116.07 mg/kg), kafeik asit (21.36 mg/kg) ve vanilin (8.77 mg/kg) gibi fenolik bileşikler olduğu saptanmıştır. Bu çalışmadan elde edilen sonuçlara göre bitkinin antioksidan kapasitesi ve fenolik madde içeriğinin belirlenmesi, bu bitkinin farmakolojik açıdan ve gıda endüstrisinde önemli bir potansiyele sahip olabileceğini ortaya koymuştur.

Anahtar Kelimeler

• Antioksidan
• Fenolik bileşik
• Iris Kerneriana
• LC-MS/MS

Antioxidant properties and phenolic components of extracts obtained from different parts of the Iris kerneriana (Çali süseni) plant

Abstract

The antioxidant properties of different parts of the Iris kerneriana Asch. & Sint. ex Baker plant in different solvents will be revealed and their phenolic content will be determined. The information obtained about the plant as a result of the study will be pioneering for use in different sectors (food, cosmetics, medicine, etc.). Revealing the content of the plant; both the elucidation of the content of the plant and the detection of the presence of phenolic substances known to have positive effects on human health are preliminary studies for the use of the plant as a medicinal aromatic plant. In the research, phenolic compounds of extracts obtained from different parts of I. kerneriana plant in different solvents were determined and their antioxidant properties were analyzed. Three different parts of the I. kerneira plant were extracted with three different solvents. The plant was evaluated according to four different analysis methods according to parts and solvents. The highest detected compounds by LC-MS/MS were found to be phenolic compounds such as catechin (187.87 mg/kg), chlorogenic acid (116.07 mg/kg), caffeic acid (21.36 mg/kg) and vanillin (8.77 mg/kg). According to the results obtained from this study, the determination of the antioxidant capacity and phenolic substance content of the plant revealed that this plant may have significant potential in terms of pharmacology and in the food industry.

Keywords

• Antioxidant
• Iris Kerneriana
• LC-MS/MS
• Phenolic compound

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