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Iris kerneriana (Çalı süseni) bitkisinin farklı kısımlarından elde edilen ekstraktların antioksidan özellikleri ve fenolik bileşenleri

Yıl 2025, Sayı: Advanced Online Publication, 703 - 713
https://doi.org/10.37908/mkutbd.1688669

Öz

Iris kerneriana Asch. & Sint. ex Baker bitkisinin farklı kısımlarının farklı çözücülerdeki antioksidan özellikleri ortaya koyulacak ve fenolik içeriği belirlenmiştir. Çalışma sonucunda bitki hakkında elde edilen bilgiler farklı sektörlerde kullanılmak üzere (gıda, kozmatik, ilaç vb) öncü olacaktır. Bitkinin içeriğinin ortaya konması; hem bitkinin içeriğinin aydınlatılması hem de insan sağlığına pozitif etkilerinin olduğu bilinen fenolik maddelerin varlığının tespiti, bitkinin tıbbi aromatik bir bitki olarak kullanımı için ön çalışma niteliğindedir. Araştırmada, I. kerneriana bitkisinin farklıkısımlarından elde edilen ekstraktların farklı çözücülerdeki fenolik bileşikleri tespit edildi ve antioksidan özellikleri analiz edildi. I. kerneira bitkisinin üç farklı kısmı üç farklı çözücü ile ekstre edildi. Bitki kısımlarına ve çözücülere göre dört farklı analiz yöntemine göre değerlendirildi. LC-MS/MS analizleriyle en yüksek düzeyde tespit edilen bileşiklerin, kateşin (187.87 mg/kg), klorojenik asit (116.07 mg/kg), kafeik asit (21.36 mg/kg) ve vanilin (8.77 mg/kg) gibi fenolik bileşikler olduğu saptanmıştır. Bu çalışmadan elde edilen sonuçlara göre bitkinin antioksidan kapasitesi ve fenolik madde içeriğinin belirlenmesi, bu bitkinin farmakolojik açıdan ve gıda endüstrisinde önemli bir potansiyele sahip olabileceğini ortaya koymuştur.

Proje Numarası

1919B012318822

Kaynakça

  • A Handbook of Garden Irises. (2009). The Group for Beardless Irises. http://www.beardlessiris.org Albuquerque, B.R., Heleno, S.A., Oliveira, M.B.P.P., Barros, L., & Ferreira, I.C.F.R. (2021). Phenolic compounds: Current industrial applications, limitations and future challenges. Food & Function, 12 (1), 14-29. https://doi.org/10.1039/D0FO02324H
  • Başer, K.H.C., Demirci, B., Orhan, I.E., Kartal, M., Şekeroglu, N., & Şener, B. (2011). Composition of volatiles from three Iris species of Turkey. Journal of Essential Oil Research, 23 (4), 66-71. https://doi.org/10.1080/10412905.2011.9700471
  • Basgedik, B., Uğur, A., & Saraç, N. (2014). Antimicrobial, antioxidant, antimutagenic activities, and phenolic compounds of Iris germanica. Industrial Crops and Products, 61, 526-530. https://doi.org/10.1016/j.indcrop.2014.07.022
  • Baytop, T. (1999). Therapy with medicinal plants in Turkey (Past and Present). Nobel Tıp Kitabevleri.
  • Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181 (4617), 1199-1200. https://doi.org/10.1038/1811199a0
  • Číž, M., Dvořáková, A., Skočková, V., & Kubala, L. (2020). The role of dietary phenolic compounds in epigenetic modulation involved in inflammatory processes. Antioxidants, 9 (8), 691. https://doi.org/10.3390/antiox9080691
  • Elmastaş, M., Gülçin, İ., Beydemir, Ş., Küfrevioğlu, Ö.İ., & Aboul‐Enein, H.Y. (2006). A study on the in vitro antioxidant activity of juniper (Juniperus communis L.) fruit extracts. Analytical Letters, 39 (1), 47-65. https://doi.org/10.1080/00032710500423385
  • Favaro, L., Balcão, V., Rocha, L., Silva, E., Oliveira Jr., J., Vila, M., & Tubino, M. (2018). Physicochemical characterization of a crude anthocyanin extract from the fruits of jussara (Euterpe edulis Martius): Potential for food and pharmaceutical applications. Journal of the Brazilian Chemical Society. https://doi.org/10.21577/0103-5053.20180082
  • Ge, L., Li, S.-P., & Lisak, G. (2020). Advanced sensing technologies of phenolic compounds for pharmaceutical and biomedical analysis. Journal of Pharmaceutical and Biomedical Analysis, 179, 112913. https://doi.org/10.1016/j.jpba.2019.112913
  • Kayır, Ö., Doğan, H., Alver, E., & Bilici, İ. (2023). Quantification of phenolic components by LC-HESI-MS/MS and evaluation of antioxidant activities of Crocus ancyrensis (Ankara çiğdemi) extracts obtained with different solvents. Chemistry & Biodiversity, 20 (4). https://doi.org/10.1002/cbdv.202201186
  • Khatib, S., Faraloni, C., & Bouissane, L. (2022). Exploring the use of Iris species: Antioxidant properties, phytochemistry, medicinal and industrial applications. Antioxidants, 11 (3), 526. https://doi.org/10.3390/antiox11030526
  • Machalska, A., Skalicka-Woźniak, K., Widelski, J., Głowniak, K., Purevsuren, G., Oyun, Z., Khishgee, D., & Urjin, B. (2008). Screening for phenolic acids in five species of Iris collected in Mongolia. Acta Chromatographica, 20 (2), 259-267. https://doi.org/10.1556/AChrom.20.2008.2.10
  • Maheshwari, N., & Sharma, M. C. (2023). Anticancer properties of some selected plant phenolic compounds: Future leads for therapeutic development. Journal of Herbal Medicine, 42, 100801. https://doi.org/10.1016/j.hermed.2023.100801
  • Mutha, R.E., Tatiya, A.U., & Surana, S.J. (2021). Flavonoids as natural phenolic compounds and their role in therapeutics: An overview. Future Journal of Pharmaceutical Sciences, 7 (1), 25. https://doi.org/10.1186/s43094-020-00161-8
  • Oyaizu, M. (1986). Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44 (6), 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  • Özderin, S. (2021). Determination of phenolic components of Vitex agnus-castus L. (Verbenaceae) from Muğla-Ula region in Turkey. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 26 (3), 692-699. https://doi.org/10.37908/mkutbd.937814
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26 (9-10), 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  • Sevindik, M., Uygun, A.E., Uysal, I., & Sabik, A.E. (2025). A comprehensive review on the biological activities, usage areas, chemical and phenolic compositions of Lycium barbarum used in traditional medicine practices. Science and Technology Indonesia, 10 (1), 173-182. https://doi.org/10.26554/sti.2025.10.1.173-182
  • Slinkard, K., & Singleton, V.L. (1977). Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28 (1), 49-55. https://doi.org/10.5344/ajev.1977.28.1.49
  • Sun, W., & Shahrajabian, M.H. (2023). Therapeutic potential of phenolic compounds in medicinal plants—Natural health products for human health. Molecules, 28 (4), 1845. https://doi.org/10.3390/molecules28041845
  • Uçan Türkmen, F., Özkan, K., Koyuncu, G., & Erol, Ü.H. (2024). Laser trilobum L., Hypericum scabrum L., ve Teucrium polium L. bitkilerinin metanol ekstraktlarının antioksidan aktiviteleri, mikrobiyolojik özellikleri ve fenolik bileşen miktarlarının belirlenmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 29 (2), 353-365. https://doi.org/10.37908/mkutbd.1398330
  • Unver, T., Uslu, H., Gurhan, I., & Goktas, B. (2024). Screening of phenolic components and antimicrobial properties of Iris persica L. subsp. persica extracts by in vitro and in silico methods. Food Science & Nutrition, 12 (9), 6578-6594. https://doi.org/10.1002/fsn3.4251
  • Yazar, M., Sevindik, M., Uysal, I., & Polat, A.O. (2025). Effects of caffeic acid on human health: Pharmacological and therapeutic effects, biological activity and toxicity. Pharmaceutical Chemistry Journal, 59 (1), 49-55. https://doi.org/10.1007/s11094-025-03363-7

Antioxidant properties and phenolic components of extracts obtained from different parts of the Iris kerneriana (Çali süseni) plant

Yıl 2025, Sayı: Advanced Online Publication, 703 - 713
https://doi.org/10.37908/mkutbd.1688669

Öz

The antioxidant properties of different parts of the Iris kerneriana Asch. & Sint. ex Baker plant in different solvents will be revealed and their phenolic content will be determined. The information obtained about the plant as a result of the study will be pioneering for use in different sectors (food, cosmetics, medicine, etc.). Revealing the content of the plant; both the elucidation of the content of the plant and the detection of the presence of phenolic substances known to have positive effects on human health are preliminary studies for the use of the plant as a medicinal aromatic plant. In the research, phenolic compounds of extracts obtained from different parts of I. kerneriana plant in different solvents were determined and their antioxidant properties were analyzed. Three different parts of the I. kerneira plant were extracted with three different solvents. The plant was evaluated according to four different analysis methods according to parts and solvents. The highest detected compounds by LC-MS/MS were found to be phenolic compounds such as catechin (187.87 mg/kg), chlorogenic acid (116.07 mg/kg), caffeic acid (21.36 mg/kg) and vanillin (8.77 mg/kg). According to the results obtained from this study, the determination of the antioxidant capacity and phenolic substance content of the plant revealed that this plant may have significant potential in terms of pharmacology and in the food industry.

Destekleyen Kurum

TUBİTAK (2209-A, Project No; 1919B012318822)

Proje Numarası

1919B012318822

Teşekkür

This study was financially supported by TUBITAK (2209-A, Project No; 1919B012318822). The authors thank for the financial support. In addition, this study has been supported by Anadolu University Scientific Research Projects Coordination Unit under grant number 2206S046.

Kaynakça

  • A Handbook of Garden Irises. (2009). The Group for Beardless Irises. http://www.beardlessiris.org Albuquerque, B.R., Heleno, S.A., Oliveira, M.B.P.P., Barros, L., & Ferreira, I.C.F.R. (2021). Phenolic compounds: Current industrial applications, limitations and future challenges. Food & Function, 12 (1), 14-29. https://doi.org/10.1039/D0FO02324H
  • Başer, K.H.C., Demirci, B., Orhan, I.E., Kartal, M., Şekeroglu, N., & Şener, B. (2011). Composition of volatiles from three Iris species of Turkey. Journal of Essential Oil Research, 23 (4), 66-71. https://doi.org/10.1080/10412905.2011.9700471
  • Basgedik, B., Uğur, A., & Saraç, N. (2014). Antimicrobial, antioxidant, antimutagenic activities, and phenolic compounds of Iris germanica. Industrial Crops and Products, 61, 526-530. https://doi.org/10.1016/j.indcrop.2014.07.022
  • Baytop, T. (1999). Therapy with medicinal plants in Turkey (Past and Present). Nobel Tıp Kitabevleri.
  • Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181 (4617), 1199-1200. https://doi.org/10.1038/1811199a0
  • Číž, M., Dvořáková, A., Skočková, V., & Kubala, L. (2020). The role of dietary phenolic compounds in epigenetic modulation involved in inflammatory processes. Antioxidants, 9 (8), 691. https://doi.org/10.3390/antiox9080691
  • Elmastaş, M., Gülçin, İ., Beydemir, Ş., Küfrevioğlu, Ö.İ., & Aboul‐Enein, H.Y. (2006). A study on the in vitro antioxidant activity of juniper (Juniperus communis L.) fruit extracts. Analytical Letters, 39 (1), 47-65. https://doi.org/10.1080/00032710500423385
  • Favaro, L., Balcão, V., Rocha, L., Silva, E., Oliveira Jr., J., Vila, M., & Tubino, M. (2018). Physicochemical characterization of a crude anthocyanin extract from the fruits of jussara (Euterpe edulis Martius): Potential for food and pharmaceutical applications. Journal of the Brazilian Chemical Society. https://doi.org/10.21577/0103-5053.20180082
  • Ge, L., Li, S.-P., & Lisak, G. (2020). Advanced sensing technologies of phenolic compounds for pharmaceutical and biomedical analysis. Journal of Pharmaceutical and Biomedical Analysis, 179, 112913. https://doi.org/10.1016/j.jpba.2019.112913
  • Kayır, Ö., Doğan, H., Alver, E., & Bilici, İ. (2023). Quantification of phenolic components by LC-HESI-MS/MS and evaluation of antioxidant activities of Crocus ancyrensis (Ankara çiğdemi) extracts obtained with different solvents. Chemistry & Biodiversity, 20 (4). https://doi.org/10.1002/cbdv.202201186
  • Khatib, S., Faraloni, C., & Bouissane, L. (2022). Exploring the use of Iris species: Antioxidant properties, phytochemistry, medicinal and industrial applications. Antioxidants, 11 (3), 526. https://doi.org/10.3390/antiox11030526
  • Machalska, A., Skalicka-Woźniak, K., Widelski, J., Głowniak, K., Purevsuren, G., Oyun, Z., Khishgee, D., & Urjin, B. (2008). Screening for phenolic acids in five species of Iris collected in Mongolia. Acta Chromatographica, 20 (2), 259-267. https://doi.org/10.1556/AChrom.20.2008.2.10
  • Maheshwari, N., & Sharma, M. C. (2023). Anticancer properties of some selected plant phenolic compounds: Future leads for therapeutic development. Journal of Herbal Medicine, 42, 100801. https://doi.org/10.1016/j.hermed.2023.100801
  • Mutha, R.E., Tatiya, A.U., & Surana, S.J. (2021). Flavonoids as natural phenolic compounds and their role in therapeutics: An overview. Future Journal of Pharmaceutical Sciences, 7 (1), 25. https://doi.org/10.1186/s43094-020-00161-8
  • Oyaizu, M. (1986). Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44 (6), 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  • Özderin, S. (2021). Determination of phenolic components of Vitex agnus-castus L. (Verbenaceae) from Muğla-Ula region in Turkey. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 26 (3), 692-699. https://doi.org/10.37908/mkutbd.937814
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26 (9-10), 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  • Sevindik, M., Uygun, A.E., Uysal, I., & Sabik, A.E. (2025). A comprehensive review on the biological activities, usage areas, chemical and phenolic compositions of Lycium barbarum used in traditional medicine practices. Science and Technology Indonesia, 10 (1), 173-182. https://doi.org/10.26554/sti.2025.10.1.173-182
  • Slinkard, K., & Singleton, V.L. (1977). Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28 (1), 49-55. https://doi.org/10.5344/ajev.1977.28.1.49
  • Sun, W., & Shahrajabian, M.H. (2023). Therapeutic potential of phenolic compounds in medicinal plants—Natural health products for human health. Molecules, 28 (4), 1845. https://doi.org/10.3390/molecules28041845
  • Uçan Türkmen, F., Özkan, K., Koyuncu, G., & Erol, Ü.H. (2024). Laser trilobum L., Hypericum scabrum L., ve Teucrium polium L. bitkilerinin metanol ekstraktlarının antioksidan aktiviteleri, mikrobiyolojik özellikleri ve fenolik bileşen miktarlarının belirlenmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 29 (2), 353-365. https://doi.org/10.37908/mkutbd.1398330
  • Unver, T., Uslu, H., Gurhan, I., & Goktas, B. (2024). Screening of phenolic components and antimicrobial properties of Iris persica L. subsp. persica extracts by in vitro and in silico methods. Food Science & Nutrition, 12 (9), 6578-6594. https://doi.org/10.1002/fsn3.4251
  • Yazar, M., Sevindik, M., Uysal, I., & Polat, A.O. (2025). Effects of caffeic acid on human health: Pharmacological and therapeutic effects, biological activity and toxicity. Pharmaceutical Chemistry Journal, 59 (1), 49-55. https://doi.org/10.1007/s11094-025-03363-7
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyokimya ve Hücre Biyolojisi (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Hacer Doğan 0000-0001-5790-8739

Ömer Kayır 0000-0002-4091-9033

Ömer Koray Yaylacı 0000-0002-1846-9646

Bahar Cömert 0009-0001-4278-4267

Proje Numarası 1919B012318822
Gönderilme Tarihi 1 Mayıs 2025
Kabul Tarihi 29 Temmuz 2025
Erken Görünüm Tarihi 3 Aralık 2025
Yayımlandığı Sayı Yıl 2025 Sayı: Advanced Online Publication

Kaynak Göster

APA Doğan, H., Kayır, Ö., Yaylacı, Ö. K., Cömert, B. (2025). Antioxidant properties and phenolic components of extracts obtained from different parts of the Iris kerneriana (Çali süseni) plant. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi(Advanced Online Publication), 703-713. https://doi.org/10.37908/mkutbd.1688669

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