ANTIMICROBIAL AND ANTIFUNGAL ACTIVITIES OF ADANA PROPOLIS

Abstract

Introduction and Aim: Propolis is a hive product that contain more than 300 active components, and the chemical compositions
and the rates of constituents varies to the geographical origin, and the plant source. The aim of this study was to investigate the
activity of Adana propolis samples against some Gram negative and Gram positive bacteria and yeastlike fungi.
Materials and Methods: The following strains were included in the peresent study: Staphylococcus aureus (ATCC 25923),
Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 12228), Enterococcus faecalis (ATTCC 29212),
Streptococcus pyogenes, Esherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Enterobacter cloacae (ATCC
13047), Proteus vulgaris, Enterobacter aerogenes, Candida albicans (ATCC 90028), Candida krusei (ATCC 6258), Candida
glabrata (ATCC 32554), Candida tropicalis (ATCC 22019) and Candida parapsilosis. The macrodilution method for antimicrobial
activity studies was performed according to the procedure of the Clinical and Laboratory Standards Institute (CLSI).
Results and Conclusion: The antimicrobial activity of propolis against to Gram positive bacteria (MIC 64-1024 ȝg/ml) was higher
both in Gram negative bacteria (MIC 256-1024 ȝg/ml) and yeast like fungi (MIC 128-1024 ȝg/ml). In addition, it was determined that
the activity of propolis against to yeast like fungi more than Gram negative bacteria. 

Keywords

• Propolis
• antibacterial activity
• antifungal activity

ADANA PROPOLİS ÖRNEKLERİNİN ANTİBAKTERİYEL VE ANTİFUNGAL ETKİSİNİN ARAŞTIRILMASI

Abstract

Giriş ve Amaç: Propolis, yapısında 300’den fazla biyolojik olarak aktif bileşeni olan ve kompozisyonu ile bileşenlerinin oranları bölgeden bölgeye ve bitki türlerine bağlı olarak değişkenlik gösteren bir arı ürünüdür. Bu çalışmada Adana orijinli propolisin bazı Gram pozitif ve Gram suşlara karşı antibakteriyel, maya formundaki mantarlara karşı ise antifungal antifungal aktivitelerinin varlığı araştırılmıştır. Gereç ve Yöntem: Çalışmada Gram pozitif suşlardan Staphylococcus aureus (ATCC 25923), Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 12228), Enterococcus faecalis (ATTCC 29212), Streptococcus pyogenes ve Gram negatif suşlardan Esherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Enterobacter cloacae (ATCC 13047), Proteus vulgaris, Enterobacter aerogenes ve maya formundaki mantarlardan ise Candida albicans (ATCC 90028), Candida krusei (ATCC 6258), Candida glabrata (ATCC 32554), Candida tropicalis (ATCC 22019) ve Candida parapsilosis kullanılmıştır. Antimikrobiyal aktivite çalışmaları makrodilüsyon yöntemi kullanılarak CLSI önerileri doğrultusunda yapılmıştır. Bulgular ve Sonuç: Propolis örneklerinin Gram pozitif bakterilere karşı antibakteriyal etkileri (MIK değeri 64-1024 ȝg/ml), Gram negatif (MIK değeri 256-1024 ȝg/ml) ve maya suşlarına karşı elde edilen antimikrobiyal aktiviteden (MIK değeri 128-1024 ȝg/ml) daha yüksek olarak bulunmuştur. Mayalara karşı etkinliğin Gram negatif bakterilere nazaran daha yüksek olduğu saptanmıştır.

Keywords

• Propolis
• antibakteriyal aktivite
• antifungal aktivite

References

1. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12: 564-82.
2. Cragg GM, Newman DJ, Snader KM.al products in drug discovery and development. Journal of Natural Products. 1997; 60: 52-60.
3. Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia. 2002; 73: 1-6.
4. Ghisalberti EL. Propolis: a review. Bee World. 1979; 60: 59-84.
5. Hegazi AG, El-Hady FKA, Abd Allah FAM.Chemical composition and antimicrobial activity of European propolis. Z. Naturforsch. 2000; 55: 70-75.
6. Sforcin JM, Fernandes AJr, Lopes CA, Bankova V, Funari SR. Seasonal effect on Brazilian propolis antibacterial activity. J. Ethnopharmacol.
7. ; 7:, 243-9.
8. Amoros M, Sauvager F, Girre L, Cormier M. In vitro antiviral activity of propolis. Apidologie. 1997; 23: 231-40.

9. Starzyk J, Scheller S, Szaflarski J, Moskwa M, Stojko A. Biological properties and clinical application of propolis. II. Studies on the antiprotozoan
10. activity of ethanol extract of propolis. Arzneimittelforschung. 1977; 27: 1198-9.
11. Banskota AH, Nagaoka T, Sumioka LY, Tezuka Y, Awale S, Midorikawa K, Matsushige K, Kadota S. Antiproliferative activity of the Netherlands
12. propolis and its active principles in cancer cell lines. J Ethnopharmacol. 2002; 80: 67-73.
13. Dimov V, Ivanovska N, Bankova V, Nikolov N, Popov S. Immunomodulatory action of propolis: IV. Prophylatic activity against Gram-negative
14. infections and adjuvant effect of water soluble derivative. Vaccine. 1992; 10: 817-23.
15. Dobrowolski JW, Vohora SB, Sharma K, Shah SA, Naqvi SAH, Dandiya PC. Antibacterial, antifungal, antiamoebic, antiinflammatory and
16. antipyretic studies on propolis bee products. J Ethnopharmacol. 1991; 35: 77-82.
17. Nagai T, Inoue R, Inoue H, Suzuki N. Preparation and antioxidant properties of water extract of propolis. Food Chem. 2003; 80: 29-33.
18. Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different
19. geographic origin. J. Ethnopharmacol. 1999; 64: 235-40.
20. Machado GM, , Leon LL, De Castro SL. Activity of Brazilian and Bulgarian propolis against different species of Leishmania. Mem Inst Oswaldo
21. Cruz. 2007; 102: 73-7.
22. -
23. Rates SMK. Plants as source of drugs. Toxicon. 2001; 39: 603-13.
24. Bankova VS, de Castro SL, Marcucci MC. Propolis; recent advantages in chemistry and plant origin. Apidologie. 2000; 31: 3-15.
25. Hegazi AG, El Hady FK. Egyptian propolis: 1- antimicrobial activity and chemical composition of Upper Egypt propolis. Z. Naturforsch. 2001; 56:
26. -8.
27. Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M, Guclu AU, Akca E, Tuylu AO, Sorkun K, Tanyuksel M, Salih B. In vitro antimicrobial activity
28. of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 2007; 13: 140-5.
29. Bosio K, Avanzini C, D’Avolio A, Ozino O, Savoia D. In vitro activity of propolis against Streptococcus pyogenes. Lett. Appl. Microbiol. 2000; 31:
30. -7.
31. Miorin PL, Levy Junior NC, Custodio AR, Bretz WA, Marcucci MC. Antibacterial activity of honey and propolis from Apis mellifera and
32. Tetragonisca angustula against Staphylococcus aureus. J. Appl. Microbiol. 2003; 95: 913-20
33. Granje JM, Davey RW. Antibakteriyal properties of propolis (bee gleu). J. of the Royal Society of Medicine. 1990; 83: 159-60.
34. Duran N, Koc A, Oksuz H, Tamer C, Akaydin Y, Kozlu T, Celik M. The protective role of topical propolis on experimental keratitis via nitric oxide
35. levels in rabbits, Mol Cell Biochem. 2006; 281: 153-61.