An overview to the cytological, physiological, genetic and morphological effects of essential oils of Mentha longifolia

Abstract

The present study was aimed to impress the cytological, physiological, genetic, and morphological effects of the essential oil obtained by hydro-distillation from Mentha longifolia (L.) HUDSON (L.) HUDSON against maize seeds. These naturally occurred compounds can be used instead of synthetic chemicals. To determine their biological activity, the oil was applied to Zea mays L. seeds at different doses. It was observed that the germination rate and mitotic division of the root tips were decreased with the increasing of the applied doses. Genomic stability was established by RAPD and the genetic differences between doses with AFLP technique. The alterations of RAPD and AFLP profiles did not support germination rate and mitotic index results while supporting each other. To determine the effects of the volatile on gene expression, the soluble protein level, and hormone levels were measured and the changes in the protein profile were determined by SDS-PAGE analyses. The independent changes were observed at hormones, soluble protein level, and protein profiles. It was concluded that the different content of oils showed a complicated effect on maize seeds. The maize seeds showed different reactions for protection.

Keywords

• AFLP
• RAPD
• SDS-Page
• Essential oil
• Lamiaceae
• Plant growth regulator

Mentha longifolia uçucu yağlarının sitolojik, fizyolojik, genetik ve morfolojik etkilerine genel bir bakış

Abstract

Bu çalışma, Mentha longifolia (L.) HUDSON (L.) HUDSON'dan hidrodistilasyon ile elde edilen uçucu yağların mısır tohumlarına karşı sitolojik, fizyolojik, genetik ve morfolojik etkilerini belirlemeyi amaçlamıştır. Doğal olarak oluşan bu bileşikler, sentetik kimyasallar yerine kullanılabilir. Uçucu yağlar, biyolojik aktivitelerinin belirlenmesi için, Zea mays L. tohumlarına farklı dozlarda uygulandı. Uygulanan yağların dozlarının artmasıyla kök ucu uzunluğunun, çimlenme hızının ve mitotik bölünmenin azaldığı gözlemlendi. Yağların neden olduğu genomik stabilite RAPD, dozlar arasındaki genetik farklılıklar ise AFLP tekniği ile belirlendi. RAPD ve AFLP profillerindeki değişikliklerin benzer olduğu ancak çimlenme oranı ve mitotik indeks sonuçlarıyla uyuşmadığı görüldü. Uçucu bileşenlerin gen ekspresyonu üzerindeki etkilerini belirlemek için çimlendirilen tohumların çözünür protein seviyesi ve bitki büyüme düzenleyicileri seviyeleri ölçüldü. Protein profillerindeki değişiklikler SDS-PAGE ile belirlendi. Bitki büyüme düzenleyicileri seviyeleri, çözünür protein seviyesi ve protein profillerinde birbirinden bağımsız değişimler gözlendi. Uygulanan uçucu yağların mısır tohumları üzerine farklı parametrelerde birbirinden bağımsız etkiler gösterdiği belirlendi. Çimlenen tohumların, ekzojen maddelerden korunmak için farklı mekanizmalar geliştirdiği kanısına varıldı.

Keywords

• Allelopati
• Bitki büyüme düzenleyicileri
• AFLP
• RAPD
• SDS-Page
• Lamiaceae
• Uçucu Yağlar

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