IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS

İmdat Aygül; Nuri Güleşçe

doi:10.18586/msufbd.81196

IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS

Abstract

Karbonik anhidraz enzimi (EC: 4.2.1.1; CA) sığır karaciğerinden saflaştırılarak bazı antibiyotiklerin inhibisyon etkileri incelendi. Saflaştırma prosedürü, homojenatın hazırlanması ve Sepharoz 4B-Tirozin-Sulfanamid Kromatografisinden oluştu. Birbirini takip eden bu iki yöntem ile
enzim 65,24 kat, % 50,86 verimle ve spesifik aktivitesi 1117.24 U/mg protein olarak saflaştırıldı. Enzim üzerine bazı antibiyotik ilaçların in vitro inhibisyon etkileri belirlendi. Bu inhibitörler için IC 50 değerleri, % aktiviteye karşı inhibitor konsantrasyon [I] grafiği çizilmek suretiyle belirlendi. Siprofloksazin ve seftazidim inhibitörlerinin IC değerleri sırasıyla 1.27 ve 5.29 mM olarak tespit 50 edildi.

Anahtar Kelimeler: Karbonik anhidraz(CA), Sığır, Karaciğer, İnhibitör, Antibiyotik

Keywords

Karbonik anhidraz(CA), Sığır, Karaciğer, İnhibitör, Antibiyotik

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

İmdat Aygül

Nuri Güleşçe This is me

Publication Date

January 1, 2015

Submission Date

August 6, 2015

Acceptance Date

-

Published in Issue

Year 2015 Volume: 3 Number: 1

DOI

https://doi.org/10.18586/msufbd.81196

IZ

https://izlik.org/JA78KH25WL

Cite

RIS / Bibtex

APA

Aygül, İ., & Güleşçe, N. (2015). IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Mus Alparslan University Journal of Science, 3(1), 334-339. https://doi.org/10.18586/msufbd.81196

AMA

1.Aygül İ, Güleşçe N. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Mus Alparslan University Journal of Science. 2015;3(1):334-339. doi:10.18586/msufbd.81196

Chicago

Aygül, İmdat, and Nuri Güleşçe. 2015. “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”. Mus Alparslan University Journal of Science 3 (1): 334-39. https://doi.org/10.18586/msufbd.81196.

EndNote

Aygül İ, Güleşçe N (November 1, 2015) IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Mus Alparslan University Journal of Science 3 1 334–339.

IEEE

[1]İ. Aygül and N. Güleşçe, “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”, Mus Alparslan University Journal of Science, vol. 3, no. 1, pp. 334–339, Nov. 2015, doi: 10.18586/msufbd.81196.

ISNAD

Aygül, İmdat - Güleşçe, Nuri. “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”. Mus Alparslan University Journal of Science 3/1 (November 1, 2015): 334-339. https://doi.org/10.18586/msufbd.81196.

JAMA

1.Aygül İ, Güleşçe N. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Mus Alparslan University Journal of Science. 2015;3:334–339.

MLA

Aygül, İmdat, and Nuri Güleşçe. “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”. Mus Alparslan University Journal of Science, vol. 3, no. 1, Nov. 2015, pp. 334-9, doi:10.18586/msufbd.81196.

Vancouver

1.İmdat Aygül, Nuri Güleşçe. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Mus Alparslan University Journal of Science. 2015 Nov. 1;3(1):334-9. doi:10.18586/msufbd.81196