IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS

İmdat Aygül; Nuri Güleşçe

doi:10.18586/msufbd.81196

IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS

Öz

Karbonik anhidraz enzimi (EC: 4.2.1.1; CA) sığır karaciğerinden saflaştırılarak bazı antibiyotiklerin inhibisyon etkileri incelendi. Saflaştırma prosedürü, homojenatın hazırlanması ve Sepharoz 4B-Tirozin-Sulfanamid Kromatografisinden oluştu. Birbirini takip eden bu iki yöntem ile
enzim 65,24 kat, % 50,86 verimle ve spesifik aktivitesi 1117.24 U/mg protein olarak saflaştırıldı. Enzim üzerine bazı antibiyotik ilaçların in vitro inhibisyon etkileri belirlendi. Bu inhibitörler için IC 50 değerleri, % aktiviteye karşı inhibitor konsantrasyon [I] grafiği çizilmek suretiyle belirlendi. Siprofloksazin ve seftazidim inhibitörlerinin IC değerleri sırasıyla 1.27 ve 5.29 mM olarak tespit 50 edildi.

Anahtar Kelimeler: Karbonik anhidraz(CA), Sığır, Karaciğer, İnhibitör, Antibiyotik

Anahtar Kelimeler

Karbonik anhidraz(CA), Sığır, Karaciğer, İnhibitör, Antibiyotik

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

-

Yazarlar

İmdat Aygül

Nuri Güleşçe Bu kişi benim

Yayımlanma Tarihi

1 Ocak 2015

Gönderilme Tarihi

6 Ağustos 2015

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2015 Cilt: 3 Sayı: 1

DOI

https://doi.org/10.18586/msufbd.81196

IZ

https://izlik.org/JA78KH25WL

Kaynak Göster

RIS / Bibtex

APA

Aygül, İ., & Güleşçe, N. (2015). IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Mus Alparslan University Journal of Science, 3(1), 334-339. https://doi.org/10.18586/msufbd.81196

AMA

1.Aygül İ, Güleşçe N. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. MAUN Fen Bil. Dergi. 2015;3(1):334-339. doi:10.18586/msufbd.81196

Chicago

Aygül, İmdat, ve Nuri Güleşçe. 2015. “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”. Mus Alparslan University Journal of Science 3 (1): 334-39. https://doi.org/10.18586/msufbd.81196.

EndNote

Aygül İ, Güleşçe N (01 Kasım 2015) IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. Mus Alparslan University Journal of Science 3 1 334–339.

IEEE

[1]İ. Aygül ve N. Güleşçe, “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”, MAUN Fen Bil. Dergi., c. 3, sy 1, ss. 334–339, Kas. 2015, doi: 10.18586/msufbd.81196.

ISNAD

Aygül, İmdat - Güleşçe, Nuri. “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”. Mus Alparslan University Journal of Science 3/1 (01 Kasım 2015): 334-339. https://doi.org/10.18586/msufbd.81196.

JAMA

1.Aygül İ, Güleşçe N. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. MAUN Fen Bil. Dergi. 2015;3:334–339.

MLA

Aygül, İmdat, ve Nuri Güleşçe. “IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS”. Mus Alparslan University Journal of Science, c. 3, sy 1, Kasım 2015, ss. 334-9, doi:10.18586/msufbd.81196.

Vancouver

1.İmdat Aygül, Nuri Güleşçe. IN VITRO INHIBITION OF BOVINE LIVER CARBONIC ANHYDRASE II BY SOME DRUGS. MAUN Fen Bil. Dergi. 01 Kasım 2015;3(1):334-9. doi:10.18586/msufbd.81196