The Relationship between IL-1β [-31 T/C] Gene Polymorphism and Symptomatic Lumbar Disc Herniation

Year 2023, , 62 - 64, 10.04.2023

Veli Çıtışlı , Aylin Koseler

https://doi.org/10.47572/muskutd.1186191

Abstract

In this study, the relationship between IL-1β [-31 C / T] gene polymorphisms in the herniated lumbar disc is offered to be investigated. IL-1β [-31 C / T] gene was amplified by the PCR [Polymerase Chain Reaction] method. Polymorphic site cut with the restriction enzyme Alu I and genotyping were performed. Fifteen subjects (15%) were homozygous for the C/C allele at -31 position of IL-1β gene. Of the remaining 85 subjects, 64 (64%) were heterozygous for C/T and 21 (21%) were homozygous for T/T. In LDH group, fifteen subjects (30%) were homozygous for the T/T allele at -31 position of IL-1β gene. 35 subjects were heterozygous for C/T (70%). There were significant differences between control and LDH group. In LDH group, C/T and T/T genotype were higher than the control group, however in control group C / C genotype was higher. C/C genotype was not determined in LDH group. In this study, IL-1β -31T allele is shown to be associated with the Lumbar herniated disc.

Keywords

Genotype, IL-1β Polymorphism, Lumbar Disc Herniation

Supporting Institution

The authors declared that this study received no financial support.

Semptomatik Lomber Disk Hernisi ile IL-1β [-31 T/C] Gen Polimorfizm İlişkisi

Abstract

Bu çalışmada fıtıklaşmış lomber diskte IL-1β [-31 C/T] gen polimorfizmleri arasındaki ilişkinin araştırılması önerilmektedir. IL-1β [-31 C/T] geni PCR [Polimeraz Zincir Reaksiyonu] yöntemi ile amplifiye edildi. Alu I restriksiyon enzimi ile polimorfik site Cut ve genotiplendirme yapıldı. On beş denek (%15), IL-1β geninin -31 konumunda C/C aleli için homozigottu. Kalan 85 denekten 64'ü (%64) C/T için heterozigot ve 21'i (%21) T/T için homozigottu. LDH grubunda on beş denek (%30) IL-1β geninin -31 pozisyonunda T/T alleli için homozigottu. 35 denek C/T için heterozigottu (%70). Kontrol ve LDH grubu arasında önemli farklılıklar vardı. LDH grubunda C/T ve T/T genotipi kontrol grubunagöre daha yüksek, kontrol grubunda ise C/C genotipi daha yüksekti. LDH grubunda C/C genotipi belirlenmedi. Bu çalışmada IL-1β -31T allelinin Lomber herniye disk ile ilişkili olduğu gösterilmiştir

Keywords

Genotip, IL-1β Polimorfizmi, Lomber Disk Hernisi

References

• 1. Andersson GB, An HS, Oegema Jr TR, et al. Directions for future research. J Bone Joint Surg Am. 2006;2:110–4.
• 2. Andersson GB. What are the age-related changes in the spine? Baillieres Clin Rheumatol. 1998;12:159–69.
• 3. Wang Z, Qu Z, Fu C, et al. İnterleukin 1 polymorphism contribute intervertebral disc degeneration risk: a meta analysis. PLos One. 2016;6:11.
• 4. Jiang H, Xiao Q, Zhang R, et al. Gene locus polymorphisms and expression levels of interleukin-1 in lumbar disc disease: a meta- analysis and immunohistochemical study. Res Sq. 2020.
• 5. Bensi G, Raugei G, Palla E, et al. Human interleukin-1 beta gene. Gene. 1987;52:95–101.
• 6. Coussens LM, Werb Z. Inflammation and cancer. Nature. 2002;420:860–7.
• 7. Aggarwal BB, Shishodia S, Sandur SK, et al. Inflammation and cancer: How hot is the link? Biochem Pharmacol. 2006;72:1605–21.
• 8. Videman T, Saarela J, Kaprio J, et al. Associations of 25 structural, degradative, and inflammatory candidate genes with lumbar disc desiccation, bulging, and height narrowing. Arthritis Rheum. 2009;60:470–481.
• 9. Solavieva S, Kouhia S, Leino-Arjas P, et al. Interleukin-1 polymorphisms and intervertebral disc degeneration. Epidemiology. 2004;15:626–33.
• 10. Aparicio JP, Bances IF, Ferna´ndez ELA, et al. The IL-1b (+3953 T/C) gene polymorphism associates to symptomatic lumbar disc herniation. Eur Spine J. 2011;3:383–9.
• 11. Pociot F, Molvig J, Wogensen L, et al. A TaqI polymorphism in the human interleukin-1 beta [IL-1 beta] gene correlates with IL-1 beta secretion in vitro. Eur J Clin Invest. 1992;22:396–402.
• 12. Guasch JF, Bertina RM, Reitsma PH. Five novel intragenic dimorphisms in the human interleukin-1 genes combine to high informativity. Cytokine. 1996;8:598–602.
• 13. Le Maitre CL, Freemont AJ, Hoyland JA. The role of interleukin-1 in the pathogenesis of human intervertebral disc degeneration. Arthritis Res Ther. 2005;7:732–45.
• 14. Karppinen J, Solovieva S, Luoma K, et al. Modic changes and interleukin 1 gene locus polymorphisms in occupational cohort of middle-aged men. Eur Spine J. 2009;18:1963–70.
• 15. Chen Y, Ma H, Bi D, et al. Association of intervertebral disc degeration risk in the Chinese Han population. Biosci Rep. 2018;4:38.
• 16. El-Omar EM, Carrington M, Chow WH, et al. Interleukin-1 polymorphisms associated with increased risk of gastric cancer. Nature. 2000;404:398–402.
• 17. Rigal J, Leglise A, Barnetche T, et al. Meta- analysis of the effects of genetic polymorphisms on intervertebral disc degeration. Eur. Spine J. 2017;26:2045–205.