İleri Evre Mide Kanserli Türk Hastalarda XPD/ERCC2, RAD51 ve hOGG1 Gen Polimorfizmi

Year 2021, Volume: 8 Issue: 1, 13 - 17, 30.04.2021

Özgür Tanrıverdi , Gamze Gokoz Dogu , Ayşegul Kargı Hakan Akça , Aydın Demiray , Burcu Yapar , Atike Gökçen Demiray , Arzu Yaren , Can Özlü , Ahmet Ergin

https://doi.org/10.47572/muskutd.676049

Abstract

Bu çalışmada ileri evre mide kanserli Türk hastalarda XPD/ERCC2, RAD51 ve hOGG1 gen polimorfizminin prognoz üzerine etkisinin olup olmadığını değerlendirdik. Çalışmaya performans durumu 0-2 olan, inoperabl lokal ileri veya uzak metastazlı 31 mide kanseri hastası ile kontrol grubu olarak 29 sağlıklı birey dahil edildi. DNA serumdan izole edildikten sonra tek nükleotid gen polimorfizmi MassARRAY Analyzer 4 System [Sequenom] kullanılarak analiz edildi. Gözlenen genotip frekanslarının istatistiksel değeri beklenen genotip frekanslarına kıyasla Hardy-Weinberg kuralına göre belirlendi. Hasta grubunda ortanca yaş 68 (yıl) ve hastaların %74’ü erkek, %26’sı kadın idi. XPD/ERCC2 Asp312Asn [rs1799793] tek nükleotit gen polimorfizmi G23591A, RAD51 [rs1801320] tek nükleotit gen polimorfizmi G135C ve hOGG1 Ser 326 Cys [rs1052133] tek nükleotid gen poliforfizmi tespit edildi. Mide kanserli Türk hastalarda genotipler ile prognoz arasında anlamlı bir ilişki kuramadık.

Keywords

Gen Polimorfizmi , Mide Kanseri , Prognoz , Tek Nükleotid Gen Polimorfizmi

Project Number

-

The XPD/ERCC2, RAD51 and hOGG1 Gene Polymorphisms in Turkish Patients with Advanced-Stage Gastric Cancer

Abstract

In this study, we evaluated the effects of XPD/ERCC2, RAD51 and hOGG1 gene polymorphisms on prognosis in Turkish patients with advanced gastric cancer. A total of 31 gastric cancer patients with locally inoperable or distant metastasis and performance status of 0–2 and 29 healthy volunteers as the control group were enrolled in this study. DNA was isolated from serum and then single nucleotide gene polymorphism analyses were done by MassARRAY Analyzer 4 System [Sequenom]. Statistical significance of the observed genotype frequencies was evaluated according to Hardy–Weinberg rule compared to the expected genotype frequencies. Median age was 68 years: 74% male, 26% female in the study group. We determined the XPD/ERCC2 Asp312Asn [rs1799793] single nucleotide gene polymorphism G23591A, RAD51 [rs1801320] single nucleotide gene polymorphism G135C and hOGG1 Ser 326 Cys [rs1052133] single nucleotide gene polymorphism. We could not find any significant association between the genotypes and prognosis in Turkish patients with gastric cancer.

Keywords

Gastric Cancer , Gene Polymorphism , Single Nucleotide Gene Polymorphism

Supporting Institution

-

Project Number

-

Thanks

-

References

• 1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2020. CA Cancer J Clin. 2020;70(1):7-30.
• 2. Chow WH, Swanson CA, Lissowska J, et al. Risk of stomach cancer in relation to consumption of cigarettes, alcohol, tea and coffee in Warsaw, Poland. Int J Cancer. 1999;81(6):871-6.
• 3. Stadtlander CT, Waterbor JW. Molecular epidemiology, pathogenesis and prevention of gastric cancer. Carcinogenesis. 1999;20(12):2195-208.
• 4. Canbay E, Agachan B, Gulluoglu M, et al. Possible associations of APE1 polymorphism with susceptibility and HOGG1 polymorphism with prognosis in gastric cancer. Anticancer Res. 2010;30(4):1359-64.
• 5. González CA, Sala N, Capellá G. Genetic susceptibility and gastric cancer risk. Int J Cancer. 2002;100(3):249-60.
• 6. Yasui W, Oue N, Kitadai Y, et al. Recent advances in molecular pathobiology of gastric carcinoma. In: The Diversity of Gastric Carcinoma. Kaminishi M, Takubo K, Mafune K [eds]. Tokyo Springer-Verlag, pp. 51-71, 2005.
• 7. Wood RD, Mitchell M, Sgouros J, et al. Human DNA repair genes. Science. 2001;291(5507):1284-9.
• 8. Oliver FJ, Menissier-de Murcia J, de Murcia G. Poly [ADP-ribose] polymerase in the cellular response to DNA damage, apoptosis, and disease. Am J Hum Genet. 1999;64(5):1282-8.
• 9. Thompson LH, Schild D. Homologous recombinational repair of DNA ensures mammalian chromosome stability. Mutat Res. 2001;477(1-2):131–53.
• 10. Neumann AS, Sturgis EM, Wei Q. Nucleotide excision repair as a marker for susceptibility to tobacco-related cancers: a review of molecular epidemiological studies. Mol Carcinog. 2005;42(2):65-92.
• 11. Sancar A, Tang MS. Nucleotide excision repair. Photochem Photobiol. 1993;57(5):905-21.
• 12. Kuznetsov SG, Haines DC, Martin BK, et al. Loss of Rad51c leads to embryonic lethality and modulation of Trp53- dependent tumorigenesis in mice. Cancer Res. 2009;69(3):863–72.
• 13. Thacker J. The RAD51 gene family, genetic instability and cancer. Cancer Lett. 2005;219(2):125–35.
• 14. Engin AB, Karahalil B, Engin A, et al. DNA repair enzyme polymorphisms and oxidative stress in a Turkish population with gastric carcinoma. Mol Biol Rep. 2011;38(8):5379-86.
• 15. Hanaoka T, Sugimura H, Nagura K, et al. hOGG1 exon7 polymorphism and gastric cancer in case-control studies of Japanese Brazilians and non-Japanese Brazilians. Cancer Lett. 2001;170(1):53-61.
• 16. Li BR, Zhou GW, Bian Q, et al. Lack of association between the hOGG1 Ser326Cys polymorphism and gastric cancer risk: a meta-analysis. Asian Pac J Cancer Prev. 2012;13(4):1145-9.
• 17. Poplawski T, Arabski M, Kozirowska D, et al. DNA damage and repair in gastric cancer-a correlation with the hOGG1 and RAD51 genes polymorphisms. Mutat Res. 2006;601(1-2):83-91.
• 18. Tsukino H, Hanaoka T, Otani T, et al. hOGG1 Ser326Cys polymorphism, interaction with environmental exposures, and gastric cancer risk in Japanese populations. Cancer Sci. 2004;95(12):977-83.
• 19. Xue H, Lu Y, Lin B, et al. The effect of XPD/ERCC2 polymorphisms on gastric cancer risk among different ethnicities: a systematic review and meta-analysis. PLoS One. 2012;7:e43431.
• 20. Capellá G, Pera G, Sala N, et al. DNA repair polymorphisms and the risk of stomach adenocarcinoma and severe chronic gastritis in the EPIC-EURGAST study. Int J Epidemiol. 2008;37(6):1316-25.
• 21. Takezaki T, Gao CM, Wu JZ, et al. hOGG1 Ser [326]Cys polymorphism and modification by environmental factors of stomach cancer risk in Chinese. Int J Cancer. 2002;99(4):624-7.
• 22. Kawabata M, Kawabata T, Nishibori M. Role of recA/RAD51 family proteins in mammals. Acta Med Okayama. 2005;59(1):1-9.
• 23. Thompson LH, West MG. XRCC1 keeps DNA from getting stranded. Mutat Res. 2000;459(1):1-18.
• 24. Lunn RM., Helzlsouer KJ, Parshad R, et al. XPD polymorphisms: effects on DNA repair proficiency. Carcinogenesis. 2000;21(4):551–5.
• 25. Chen Z, Zhang C, Xu C, et al. Effects of selected genetic polymorphisms in xeroderma pigmentosum complementary group D on gastric cancer. Mol Biol Rep. 2011;38(3):1507–13.
• 26. Yuan T, Deng S, Chen M, et al. Association of DNA repair gene XRCC1 and XPD polymorphisms with genetic susceptibility to gastric cancer in a Chinese population. Cancer Epidemiol. 2011;35(2):170–4.