The XPD/ERCC2, RAD51 and hOGG1 Gene Polymorphisms in Turkish Patients with Advanced-Stage Gastric Cancer

Yıl 2021, Cilt: 8 Sayı: 1, 13 - 17, 30.04.2021

Özgür Tanrıverdi , Gamze Gokoz Dogu , Ayşegul Kargı Hakan Akça , Aydın Demiray , Burcu Yapar , Atike Gökçen Demiray , Arzu Yaren , Can Özlü , Ahmet Ergin

https://doi.org/10.47572/muskutd.676049

Öz

In this study, we evaluated the effects of XPD/ERCC2, RAD51 and hOGG1 gene polymorphisms on prognosis in Turkish patients with advanced gastric cancer. A total of 31 gastric cancer patients with locally inoperable or distant metastasis and performance status of 0–2 and 29 healthy volunteers as the control group were enrolled in this study. DNA was isolated from serum and then single nucleotide gene polymorphism analyses were done by MassARRAY Analyzer 4 System [Sequenom]. Statistical significance of the observed genotype frequencies was evaluated according to Hardy–Weinberg rule compared to the expected genotype frequencies. Median age was 68 years: 74% male, 26% female in the study group. We determined the XPD/ERCC2 Asp312Asn [rs1799793] single nucleotide gene polymorphism G23591A, RAD51 [rs1801320] single nucleotide gene polymorphism G135C and hOGG1 Ser 326 Cys [rs1052133] single nucleotide gene polymorphism. We could not find any significant association between the genotypes and prognosis in Turkish patients with gastric cancer.

Anahtar Kelimeler

Gastric Cancer, Gene Polymorphism, Single Nucleotide Gene Polymorphism

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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İleri Evre Mide Kanserli Türk Hastalarda XPD/ERCC2, RAD51 ve hOGG1 Gen Polimorfizmi

Öz

Bu çalışmada ileri evre mide kanserli Türk hastalarda XPD/ERCC2, RAD51 ve hOGG1 gen polimorfizminin prognoz üzerine etkisinin olup olmadığını değerlendirdik. Çalışmaya performans durumu 0-2 olan, inoperabl lokal ileri veya uzak metastazlı 31 mide kanseri hastası ile kontrol grubu olarak 29 sağlıklı birey dahil edildi. DNA serumdan izole edildikten sonra tek nükleotid gen polimorfizmi MassARRAY Analyzer 4 System [Sequenom] kullanılarak analiz edildi. Gözlenen genotip frekanslarının istatistiksel değeri beklenen genotip frekanslarına kıyasla Hardy-Weinberg kuralına göre belirlendi. Hasta grubunda ortanca yaş 68 (yıl) ve hastaların %74’ü erkek, %26’sı kadın idi. XPD/ERCC2 Asp312Asn [rs1799793] tek nükleotit gen polimorfizmi G23591A, RAD51 [rs1801320] tek nükleotit gen polimorfizmi G135C ve hOGG1 Ser 326 Cys [rs1052133] tek nükleotid gen poliforfizmi tespit edildi. Mide kanserli Türk hastalarda genotipler ile prognoz arasında anlamlı bir ilişki kuramadık.

Anahtar Kelimeler

Gen Polimorfizmi, Mide Kanseri, Prognoz, Tek Nükleotid Gen Polimorfizmi

Proje Numarası

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Kaynakça

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