Development of IrO2 Nanoparticles-Based Lateral Flow Immunosensor for Determination of Parathyroid Hormone

Year 2025, Volume: 7 Issue: 2, 286 - 293, 31.08.2025

Eda Gümüş , Haluk Bingöl , Erhan Zor

Abstract

The conveniences that biosensors bring to our lives pave the way for new studies that will enable biosensor technology to develop day by day. Qualitative and quantitative analyses are conducted in many areas with optical sensors. Among these areas, hormone tests, which are important in terms of health, also attract attention. This study aims to detect parathyroid hormone (PTH), which has an important role in some metabolic activities by maintaining calcium balance in the blood, by paper-based lateral flow analysis. PTH levels in the blood are important for the diagnosis of many parathyroid-related diseases and cancer. PTH was determined by the developed iridium oxide nanoparticles-based (IrO2NPs) LFA. At the same time, this is the first study to develop an LFA for the detection of PTH using IrO2NPs. The limit of detection and quantification (LOD and LOQ) of IrO2NPs-based LFA designed for PTH determination were calculated as 3.92 ng/mL and 11.88 ng/mL, respectively.

Keywords

Lateral flow immunosensor , Paper-based biosensor , Iridium oxide nanoparticle , Parathyroid hormone

Supporting Institution

Necmettin Erbakan University, Scientific Research Projects Coordination Unit (NEU-BAP)

Project Number

201210004

Thanks

Authors thanks to Necmettin Erbakan University, Scientific Research Projects Coordination Unit (NEU-BAP) for financial support (201210004).

Paratiroid Hormon Tayinine Yönelik IrO2 Nanopartikül Tabanlı Yatay Akış İmmünosensör Geliştirilmesi

Abstract

Biyosensörlerin hayatımıza getirdiği kolaylıklar, biyosensör teknolojisinin gün geçtikçe gelişmesini sağlayacak yeni çalışmaların önünü açmaktadır. Optik sensörler ile birçok alanda kalitatif ve kantitatif analizler yapılmaktadır. Bu alanlar arasında sağlık sektöründe önemli olan hormon testleri de dikkat çekmektedir. Bu çalışmada, kandaki kalsiyum dengesini koruyarak bazı metabolik faaliyetlerde önemli bir role sahip olan paratiroid hormonunun (PTH) kağıt bazlı yatay akış testi (LFA) ile tayin edilmesi amaçlanmıştır. Kandaki PTH seviyesi birçok paratiroid kaynaklı hastalık ve kanser teşhisi için önem arz etmektedir. Geliştirilen iridyum oksit nanopartikül (IrO2NPs) esaslı LFA ile PTH tayini ve tespiti gerçekleştirilmiştir. Aynı zamanda, PTH tespiti için IrO2NPs kullanılarak LFA geliştirilen ilk çalışmadır. PTH tayini için tasarlanan IrO2NPs bazlı LFA’nın teşhis ve tayin alt sınırı (LOD ve LOQ) sırasıyla 3,92 ng/mL ve 11,88 ng/mL olarak hesaplanmıştır.

Keywords

Yatay akış immünosensör , Kağıt bazlı biyosensör , İridyum oksit nanopartikül , Paratiroid hormon

Supporting Institution

Necmettin Erbakan Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi (NEÜ-BAP)

Project Number

201210004

Thanks

Yazarlar, maddi desteklerinden dolayı Necmettin Erbakan Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'ne (NEÜ-BAP) teşekkür eder (201210004).

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