Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant

Abstract

In this paper we reported the effect of different cytokinins on callus induction and adventitious shoot regeneration from leaf segments of in vitro grown Centaurea zeybekii Wagenitz seedlings.

Callus was not induced when the explants were cultured on MS medium devoid of any growth regulators. Callus formation was observed in MS media containing TDZ, BA and KIN. The highest callusing response was observed in the media containing 0.005 mg/Land 0.01 mg/LTDZ (100%). In adventitious shoot induction experiments, well developed shoots were observed only MS media supplemented with KIN at the end of sixth week. The highest number of shoot per explant was obtained from the MS medium containing 1 mg/LKIN (6.2 shoots/explant). The highest average of maximum shoot length was also observed in this medium (4.17 cm). There was no difference between MS and ½ MS media in rooting but it was very low and only 15 % of total shoots could rooted in medium containing 0.5 mg/LIBA.

Keywords

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Tehlike Altındaki Endemik Bitki Centaurea zeybekii Wagenitz ‘nin Kallus İndüksiyonu

Abstract

Bu çalışmada in vitro büyütülmüş Centaurea zeybekii Wagenitz fidelerinin yaprak parçalarından kallus indüksiyonu ve adventif sürgün rejenerasyonu üzerine farklı sitokininlerin etkisi rapor edilmiştir. Eksplantlar herhangi bir büyüme düzenleyicisi içermeyen MS ortamına kültüre edildikleri zaman kallus oluşumu gerçekleşmemiştir. Kallus oluşumu TDZ, BA ve KIN içeren MS ortamlarında gözlenmiştir. En yüksek kalluslaşma cevabı 0.005 mg/L ve 0.01 mg/LTDZ (% 100) içeren ortamlarda gözlenmiştir. Adventif sürgün indüksiyonu denemelerinde, iyi gelişmiş sürgünler altıncı haftanın sonunda sadece KIN içeren MS ortamında gözlenmiştir. Eksplant başına en yüksek sürgün sayısı 1 mg/LKIN (6.2 sürgün/eksplant) içeren MS ortamından elde edilmiştir. Maksimum sürgün boyunun en yüksek ortalaması da aynı ortamda gözlenmiştir (4.17 cm). Köklenme açısından MS ve ½ MS ortamları arasında fark yoktur. Ancak köklenme çok düşüktür ve total sürgünlerin sadece % 15’i 0.5 mg/LIBA içeren ortamda köklendirilebilmişlerdir

Keywords

• Centaurea zeybekii, endemik, kallus, adventif sürgün

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