BibTex RIS Kaynak Göster

Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant

Yıl 2015, , 1 - 8, 08.01.2016
https://doi.org/10.17100/nevbiltek.210944

Öz

In this paper we reported the effect of different cytokinins on callus induction and adventitious shoot regeneration from leaf segments of in vitro grown Centaurea zeybekii Wagenitz seedlings.

Callus was not induced when the explants were cultured on MS medium devoid of any growth regulators. Callus formation was observed in MS media containing TDZ, BA and KIN. The highest callusing response was observed in the media containing 0.005 mg/Land 0.01 mg/LTDZ (100%). In adventitious shoot induction experiments, well developed shoots were observed only MS media supplemented with KIN at the end of sixth week. The highest number of shoot per explant was obtained from the MS medium containing 1 mg/LKIN (6.2 shoots/explant). The highest average of maximum shoot length was also observed in this medium (4.17 cm). There was no difference between MS and ½ MS media in rooting but it was very low and only 15 % of total shoots could rooted in medium containing 0.5 mg/LIBA.

Kaynakça

  • Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z., Adıgüzel, N., “Türkiye Bitkileri Kırmızı Kitabı (Egrelti ve Tohumlu Bitkiler)”, Türkiye Tabiatını Koruma Dernegi, Ankara, 2000.
  • Fay, M.F., “Conservation of rare and endangered plants using in vitro methods” In vitro Cellular & Developmental Biology-Plant, 28, 1-4, 1992.
  • Fay, M.F., “In what situation is in vitro culture appropriate to plant conservation?” Biodiversity and Conservation, 3, 176-183, 1994.
  • Irıando, J.M., Perez C., “Application of in vitro culture techniques to the conservation of Iberian endemic endangered plant species” Botanic Gardens Micropropagation News, 1, 4-6, 1990.
  • Wochock, Z.S., “The role of tissue culture in preserving threatened and endangered plant species” Biological Conservation, 20, 83-89, 1981.
  • Cuenca, S., Amo- Marco, J.B., Para, R., “Micropropagation from inflorescense stems of the Spanish endemic plant Centaurea paui Loscos ex Wilk. (Compositae)” Plants Cell Reports, 18, 674-679, 1998.
  • Hammat, N., Evans, P.K., “The in vitro propagation of an endangered species: Centaurea junoniana Svent. (Compositae)” Horticultural Science, 60, 93-97, 1985.
  • Iriondo J.M., Perez C., “Micropropagation and in vitro strage of Centaurium rigualii Esteve (Gentianaceae)” Israel Journal of Plant Sciences, 44, 115-123, 1996.
  • Kurt S., Erdağ B., “In vitro germination and axillary shoot propagation of Centaurea zeybekii” Biologia-Section Botany, 64, 97-101, 2009.
  • Gurbuz I., Yesilada E., “Evaluation of antiulcerogenic effect of sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis by using various in vivo and biochemical techniques” Journal of Ethnopharmacology, 112, 284- 91, 2007.
  • Robles M., Wang N., Kim R., Choi B.H., “Cytotoxic effects of repin, a principal sesquiterpene lactone of Russian knapweed” Journal of Neuroscience Research, 47, 90- 97, 1997.
  • Rusak G., Krajac’ic M., Ples’e N., “ Inhibition of tomato bushy stunt virus infection using a quercetagetin flavonoid isolated from Centaurea rupestris L” Antiviral Research, 36, 125-129, 1997.
  • Altıntaş A., Tugay B., Demirci K., Başer H.C., “Composition of the essential oils of Centaurea diffusa Lam. and Centaurea zeybekii Wagenitz” WOCMAP IV (World Conference on Medicinal and Aromatic Compounds), 9-14 November, Abstract Book, 477 pp, Cape Town, South Africa, 2008.
  • Kurt S., Erdağ B., Karagözler A.A., Uygun M., Centaurea zeybekii Wagenitz (Asteraceae)’nin antioksidan aktivitesi üzerine bir araştırma” 19. Ulusal Biyoloji Kongresi, 23-27 Haziran, Bildir özet kitabı, s. 413, Trabzon, 2008.
  • Murashige T., Skoog F., “A revised medium for rapid growth and bioassays with tobacco tissue culture” Plant Physiology, 15, 437-497, 1962.
  • Merciey H., Kerbauy G.B., 1997 “Micropropagation of ornamental bromeliads (Bromeliaceae)” Biotechnology in Agriculture and Forestry, 40, 43–57, 1997.
  • Babaoğlu M., Gurel E., Ozcan S., “Plant Biotechnology, Tissue Culture and Application”, Selçuk University Press, Turkey, pp. 374, 2002.
  • Ikeuchi M., Sugimoto K., Iwase A., “Plant Callus: Mechanisms of Induction and Repression” The Plant Cell, 25, 3159–3173, 2013.
  • Cölgecen H., Koca U., Yagcı C., Toker G., Toker MC., “Callus induction and secondary metabolite production studies in Centaurea tchihatcheffii Fisch. & C. A. Mey” Journal of Pharmaceutical Sciences, 37, 9-16, 2012.
  • Kairong C., Ji L., Gengmei X., Jianlong L., Lihong W., Yafu W., “Effect of hydrogen peroxide on
  • synthesis of proteins during somatic embryogenesis in Lycium barbarum” Plant Cell Tissue & Organ Culture, 68, 187–193, 1999.
  • Emek Y., Erdag B.,” In vitro somatic embryogenesis and antioxidant enzyme activities in Rhaponticoides mykalea (Hub.-Mor.) M. V. Agab. & Greuter critically endangered endemic plant” Fresenius Environmental Bulletin, 23, 1051-1057, 2014.
  • Luczkiewicz M., Zarate R., Dembinska-Migas W., Migas P., Verpoorte R., “Production of pulchelin E in hairy roots, callus and suspension cultures of Rudbeckia hirta L.” Plant Science, 163, 91-100, 2002.
  • Luczkiewicz M., Glod D., “Callus cultures of Genista plants - in vitro material producing high amounts of isoflavones of phytoestrogenic activity” Plant Science, 165, 1101-1108, 2003.
  • Ozel C.A., Khawar K. M., Mirici S., Ozcan S., Arslan O., “Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey.) Naturwissenschaften 93, 511–517, 2006.

Tehlike Altındaki Endemik Bitki Centaurea zeybekii Wagenitz ‘nin Kallus İndüksiyonu

Yıl 2015, , 1 - 8, 08.01.2016
https://doi.org/10.17100/nevbiltek.210944

Öz

Bu çalışmada in vitro büyütülmüş Centaurea zeybekii Wagenitz fidelerinin yaprak parçalarından kallus indüksiyonu ve adventif sürgün rejenerasyonu üzerine farklı sitokininlerin etkisi rapor edilmiştir. Eksplantlar herhangi bir büyüme düzenleyicisi içermeyen MS ortamına kültüre edildikleri zaman kallus oluşumu gerçekleşmemiştir. Kallus oluşumu TDZ, BA ve KIN içeren MS ortamlarında gözlenmiştir. En yüksek kalluslaşma cevabı 0.005 mg/L ve 0.01 mg/LTDZ (% 100) içeren ortamlarda gözlenmiştir. Adventif sürgün indüksiyonu denemelerinde, iyi gelişmiş sürgünler altıncı haftanın sonunda sadece KIN içeren MS ortamında gözlenmiştir. Eksplant başına en yüksek sürgün sayısı 1 mg/LKIN (6.2 sürgün/eksplant) içeren MS ortamından elde edilmiştir. Maksimum sürgün boyunun en yüksek ortalaması da aynı ortamda gözlenmiştir (4.17 cm). Köklenme açısından MS ve ½ MS ortamları arasında fark yoktur. Ancak köklenme çok düşüktür ve total sürgünlerin sadece % 15’i 0.5 mg/LIBA içeren ortamda köklendirilebilmişlerdir

Kaynakça

  • Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z., Adıgüzel, N., “Türkiye Bitkileri Kırmızı Kitabı (Egrelti ve Tohumlu Bitkiler)”, Türkiye Tabiatını Koruma Dernegi, Ankara, 2000.
  • Fay, M.F., “Conservation of rare and endangered plants using in vitro methods” In vitro Cellular & Developmental Biology-Plant, 28, 1-4, 1992.
  • Fay, M.F., “In what situation is in vitro culture appropriate to plant conservation?” Biodiversity and Conservation, 3, 176-183, 1994.
  • Irıando, J.M., Perez C., “Application of in vitro culture techniques to the conservation of Iberian endemic endangered plant species” Botanic Gardens Micropropagation News, 1, 4-6, 1990.
  • Wochock, Z.S., “The role of tissue culture in preserving threatened and endangered plant species” Biological Conservation, 20, 83-89, 1981.
  • Cuenca, S., Amo- Marco, J.B., Para, R., “Micropropagation from inflorescense stems of the Spanish endemic plant Centaurea paui Loscos ex Wilk. (Compositae)” Plants Cell Reports, 18, 674-679, 1998.
  • Hammat, N., Evans, P.K., “The in vitro propagation of an endangered species: Centaurea junoniana Svent. (Compositae)” Horticultural Science, 60, 93-97, 1985.
  • Iriondo J.M., Perez C., “Micropropagation and in vitro strage of Centaurium rigualii Esteve (Gentianaceae)” Israel Journal of Plant Sciences, 44, 115-123, 1996.
  • Kurt S., Erdağ B., “In vitro germination and axillary shoot propagation of Centaurea zeybekii” Biologia-Section Botany, 64, 97-101, 2009.
  • Gurbuz I., Yesilada E., “Evaluation of antiulcerogenic effect of sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis by using various in vivo and biochemical techniques” Journal of Ethnopharmacology, 112, 284- 91, 2007.
  • Robles M., Wang N., Kim R., Choi B.H., “Cytotoxic effects of repin, a principal sesquiterpene lactone of Russian knapweed” Journal of Neuroscience Research, 47, 90- 97, 1997.
  • Rusak G., Krajac’ic M., Ples’e N., “ Inhibition of tomato bushy stunt virus infection using a quercetagetin flavonoid isolated from Centaurea rupestris L” Antiviral Research, 36, 125-129, 1997.
  • Altıntaş A., Tugay B., Demirci K., Başer H.C., “Composition of the essential oils of Centaurea diffusa Lam. and Centaurea zeybekii Wagenitz” WOCMAP IV (World Conference on Medicinal and Aromatic Compounds), 9-14 November, Abstract Book, 477 pp, Cape Town, South Africa, 2008.
  • Kurt S., Erdağ B., Karagözler A.A., Uygun M., Centaurea zeybekii Wagenitz (Asteraceae)’nin antioksidan aktivitesi üzerine bir araştırma” 19. Ulusal Biyoloji Kongresi, 23-27 Haziran, Bildir özet kitabı, s. 413, Trabzon, 2008.
  • Murashige T., Skoog F., “A revised medium for rapid growth and bioassays with tobacco tissue culture” Plant Physiology, 15, 437-497, 1962.
  • Merciey H., Kerbauy G.B., 1997 “Micropropagation of ornamental bromeliads (Bromeliaceae)” Biotechnology in Agriculture and Forestry, 40, 43–57, 1997.
  • Babaoğlu M., Gurel E., Ozcan S., “Plant Biotechnology, Tissue Culture and Application”, Selçuk University Press, Turkey, pp. 374, 2002.
  • Ikeuchi M., Sugimoto K., Iwase A., “Plant Callus: Mechanisms of Induction and Repression” The Plant Cell, 25, 3159–3173, 2013.
  • Cölgecen H., Koca U., Yagcı C., Toker G., Toker MC., “Callus induction and secondary metabolite production studies in Centaurea tchihatcheffii Fisch. & C. A. Mey” Journal of Pharmaceutical Sciences, 37, 9-16, 2012.
  • Kairong C., Ji L., Gengmei X., Jianlong L., Lihong W., Yafu W., “Effect of hydrogen peroxide on
  • synthesis of proteins during somatic embryogenesis in Lycium barbarum” Plant Cell Tissue & Organ Culture, 68, 187–193, 1999.
  • Emek Y., Erdag B.,” In vitro somatic embryogenesis and antioxidant enzyme activities in Rhaponticoides mykalea (Hub.-Mor.) M. V. Agab. & Greuter critically endangered endemic plant” Fresenius Environmental Bulletin, 23, 1051-1057, 2014.
  • Luczkiewicz M., Zarate R., Dembinska-Migas W., Migas P., Verpoorte R., “Production of pulchelin E in hairy roots, callus and suspension cultures of Rudbeckia hirta L.” Plant Science, 163, 91-100, 2002.
  • Luczkiewicz M., Glod D., “Callus cultures of Genista plants - in vitro material producing high amounts of isoflavones of phytoestrogenic activity” Plant Science, 165, 1101-1108, 2003.
  • Ozel C.A., Khawar K. M., Mirici S., Ozcan S., Arslan O., “Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey.) Naturwissenschaften 93, 511–517, 2006.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Biyoloji
Yazarlar

Serap Aydoğan Bu kişi benim

Bengi Erdağ

Yayımlanma Tarihi 8 Ocak 2016
Yayımlandığı Sayı Yıl 2015

Kaynak Göster

APA Aydoğan, S., & Erdağ, B. (2016). Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant. Nevşehir Bilim Ve Teknoloji Dergisi, 4(2), 1-8. https://doi.org/10.17100/nevbiltek.210944
AMA Aydoğan S, Erdağ B. Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant. Nevşehir Bilim ve Teknoloji Dergisi. Ocak 2016;4(2):1-8. doi:10.17100/nevbiltek.210944
Chicago Aydoğan, Serap, ve Bengi Erdağ. “Callus Induction and Adventitious Shoot Regeneration of Centaurea Zeybekii Wagenitz: Endangered Endemic Plant”. Nevşehir Bilim Ve Teknoloji Dergisi 4, sy. 2 (Ocak 2016): 1-8. https://doi.org/10.17100/nevbiltek.210944.
EndNote Aydoğan S, Erdağ B (01 Ocak 2016) Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant. Nevşehir Bilim ve Teknoloji Dergisi 4 2 1–8.
IEEE S. Aydoğan ve B. Erdağ, “Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant”, Nevşehir Bilim ve Teknoloji Dergisi, c. 4, sy. 2, ss. 1–8, 2016, doi: 10.17100/nevbiltek.210944.
ISNAD Aydoğan, Serap - Erdağ, Bengi. “Callus Induction and Adventitious Shoot Regeneration of Centaurea Zeybekii Wagenitz: Endangered Endemic Plant”. Nevşehir Bilim ve Teknoloji Dergisi 4/2 (Ocak 2016), 1-8. https://doi.org/10.17100/nevbiltek.210944.
JAMA Aydoğan S, Erdağ B. Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant. Nevşehir Bilim ve Teknoloji Dergisi. 2016;4:1–8.
MLA Aydoğan, Serap ve Bengi Erdağ. “Callus Induction and Adventitious Shoot Regeneration of Centaurea Zeybekii Wagenitz: Endangered Endemic Plant”. Nevşehir Bilim Ve Teknoloji Dergisi, c. 4, sy. 2, 2016, ss. 1-8, doi:10.17100/nevbiltek.210944.
Vancouver Aydoğan S, Erdağ B. Callus Induction and Adventitious Shoot Regeneration of Centaurea zeybekii Wagenitz: Endangered Endemic Plant. Nevşehir Bilim ve Teknoloji Dergisi. 2016;4(2):1-8.

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