IL-10 and IL-17 Gene Polymorphisms in Adult Idiopathic Thrombocytopenic Purpura

Abstract

Objective: Interleukin 10 (IL-10) and interleukin 17 (il-17) cytokine gene polymorphisms were investigated in many autoimmune diseases and there were significant results in some studies but there were a few published studies on IL-10 and il-17 gene polymorphisms in idiopathic thrombocytopenic purpura ( ITP). In this study, we aimed to evaluate the IL-10 (-592 A/C) and IL-17 (A126G) gene polymorphisms and their serum levels in adult patients with ITP.

Materials and Methods: The IL-10 (-592 A/C) and IL-17 (A126G) gene polymorphisms in 50 patients with ITP and 51 healthy control groups were determined after DNA isolation and serum levels of these cytokines were measured by ELISA method in 32 patients with ITP and 32 healthy volunteers.

Results: There was no statistically significant difference in IL-10 (-592 A/C) and IL-17 (A126G) gene polymorphisms between the patients and control groups, whereas serum IL-10 levels were found to be statistically significant higher in ITP patients than control group (p=0.003). Serum IL-17 levels were similar in patients and control groups. In addition, age, sex, response to treatment and platelet values at diagnosis were compared between the groups, but there was no statistically significant difference between the groups.

Conclusion: In our study, IL-10 (-592 A/C) and IL-17 (A126G) gene polymorphisms were not found to be different in patients with ITP compared to healthy subjects, although IL-10 levels were found to be higher in the ITP group than healthy group. When we look the datas in the literature, we think that more studies with larger series are needed in this field in order to clarify the importance of cytokines in the diagnosis and follow-up of ITP.

Keywords

• Immune thrombocytopenic purpura,interleukin-10,interleukin -17

Erişkin İdiyopatik Trombositopenik Purpuralı Hastalarda IL-10 ve IL-17 Gen Polimorfizm

Öz

Amaç: İnterlökin 10 (IL-10) ve interlökin 17 (IL-17) sitokin gen polimorfizmlerinin otoimmün birçok hastalıkta araştırıldığı ve bazılarında anlamlı sonuçlar bulunduğu ancak idiyopatik trombositopenik purpuralı (ITP) hastalarda IL-10 ve IL-17 gen polimorfizmleri ile ilgili yayınlanmış az sayıda çalışma mevcut olduğu görülmüştür. Bu çalışmada erişkin ITP hastalarında IL-10 (-592 A/C) ve IL-17 (A126G) gen polimorfizmi ve bu sitokinlerin serum düzeylerinin değerlendirilmesi amaçlanmıştır.

Materyal ve Metot: 50 ITP’ li hasta ve 51 sağlıklı kontrol grubunda IL-10 (-592 A/C) ve IL-17 (A126G) gen polimorfizmleri, DNA izolasyonu yapıldıktan sonra belirlenmiş ve bu sitokinlerin serum düzeyleri ELİSA yöntemi ile toplam 32 ITP’li hastada ve 32 sağlıklı kişide ölçülmüştür.

Bulgular: Hasta ve kontrol grupları arasında IL-10 (-592 A/C) ve IL-17 (A126G) gen polimorfizmleri dağılımı açısından istatistiksel olarak anlamlı fark bulunmazken, serum IL-10 düzeyleri ITP’li hastalarda sağlıklı bireylere göre istatistiksel olarak anlamlı derecede yüksek bulunmuştur (p=0,003). Serum IL-17 düzeyleri ise hasta ve kontrol grubunda benzer bulunmuştur. Ayrıca gruplar arasında yaş, cinsiyet, tedavi cevabı, tanı anıdaki trombosit değerleri karşılaştırılmış ancak gruplar arasında istatistiksel olarak anlamlı fark saptanmamıştır.

Sonuç: Çalışmamızda ITP’li hastalarda sağlıklı kişilere göre IL-10 (-592 A/C) ve IL-17 (A126G) gen polimorfizmleri sıklıklarının farklı olduğu saptanamasa da IL-10 düzeyleri ITP’li grupta yüksek bulunmuştur. Sitokinlerin ITP’nin tanı ve takibindeki önemini açıklığa kavuşturabilmek için literatürdeki veriler de dikkate alındığında bu alanda daha geniş hasta serili çalışmalara ihtiyaç olduğunu düşünmekteyiz. 

Anahtar Kelimeler

• İmmun trombositopenik purpura,İnterlökin-10,İnterlökin -17

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