Evaluation of the chemical composition and biological activities of Salvia officinalis subsp. lavandulifolia (Vahl) Gams essential oil

Year 2018, Volume: 5 Issue: 3, 1 - 6, 30.12.2018

Gözde Öztürk Betül Demirci Fatih Demirci

Abstract

The main aim of the present study was to
investigate the antibacterial effect of the commercial Pharma Grade Salvia lavandulifolia Vahl. (Lamiaceae)
essential oil against the skin pathogen
Staphylococcus aureus. The chemical composition of the essential oil was
confirmed by GC and GC/MS, simultaneously. Camphor (30.5%), 1,8-cineole
(24.8%), α-pinene (6.5%), linalool
(4.0%) and linalyl acetate (3.5%) were found as major components, respectively.
The bioactivity of the essential oil and its main compounds were tested using
the in vitro microdilution technique.
Minimum inhibitory concentration (MIC) values of α-pinene, essential oil, camphor, linalool and linalyl acetate were
in the range of 2.5-10 mg/mL, respectively. The results showed that the tested
pathogen was only moderately susceptible against the essential oil and its main
compounds when compared with standard antibiotics. In addition, the in vitro antioxidant activity was
evaluated using the radical scavenging activity mediated by
1,1-diphenyl-2-picrylhydrazyl (DPPH). The activity range was found more than 30
mg/mL for all tested oil samples, compared with the standard. The results
suggested that the oil and its major constituents were rather weak
antimicrobial agent supporting its antiseptic folk medicinal use.

Keywords

Salvia lavandulifolia Vahl., essential oil, antibacterial activity, antioxidant

References

• Anonymous, (2006). Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, CLSI M7-A7, Clinical and Laboratory Standards Institute. Pennsylvania.
• Anonymous, (2016). Council of Europe. European Pharmacopoeia. 9th ed. Strasbourg: Council of Europe. Chambers, H.F. & Deleo, F.R. (2009). Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol., 7(9), 629-641.
• Cutillas, A.B., Carrasco, A., Martinez-Gutierrez, R., Tomas, V. & Tudela, J. (2017). Composition and antioxidant, antienzymatic and antimicrobial activities of volatile molecules from Spanish Salvia lavandulifolia (Vahl) essential oils. Molecules, 22, 1382-1397.
• Deleo, F.R., Otto M., Kreiswirth, B.N. & Chambers, H.F. (2010). Community-associated methicillin resistant Staphylococcus aureus. Lancet. 375(9725), 1557-1568.
• Demirci, F., Bayramiç, P., Göger, G., Demirci, B. & Baser, K.H.C., (2015). Characterization and antimicrobial evaluation of the essential oil of Pinus pinea L. from Turkey. Nat. Volat. Essent. Oils, 2(2), 39-44.
• Demirci, F., Güven, K., Demirci, B., Dadandı, M.Y. & Baser, K.H.C., (2008). Antibacterial activity of two Phlomis essential oils against food pathogens. Food Cont. 19(12), 1159-1164.
• Fanelli, M.D.; Kupperman, B.A., Edelstein, P.H. & Margolis, D.J. (2011). Antibiotics, acne, and Staphylococcus aureus colonization. Arch. Dermatol., 147(8), 917-921.
• Joulain, D. & König, W. A., (1998). The Atlas of Spectra Data of Sesquiterpene Hydrocarbons. University of Hamburg. E. B. Verlag, Hamburg.
• Kintzios, E. S. (2000). Sage: the Genus Salvia, Harwood Academic Publishers, The Netherlands.
• Kumarasamy, Y., Byres, M., Cox, P. J., Jaspars, M., Nahar, L. & Sarker, S. D. (2007). Screening seeds of some Scottish plants for free radical scavenging activity. Phytotherapy Research, 21, 615-621.
• McLafferty, F. W. & Stauffer, D. B., (1989). The Wiley/NBS Registry of Mass Spectral Data. J. Wiley and Sons.
• Nikolic, M., Jovanovic, K.K., Markovic, T., Markovic, D. Gligorijevic, N., Radulovic, S. & Sokovic, M. (2014). Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils. Industrial Crops and Products, 61, 225-232.
• Perry, N. S., Bollen, C., Perry, E. K. & Ballard, C. (2003). Salvia for dementia therapy: Review of pharmacological activity and pilot tolerability clinical trial. Pharmacology, Biochemistry, and Behaviour, 75, 651–659.
• Pierozan, M.K., Pauletti, G.F., Rota, L., Santos, A.C.A., Lerin, L.A., Di Luccio, M., Mossi, A.J., Atti-Serafini, L., Cansian, R.L., & Vladimir Oliveira, J. (2009). Chemical characterization and antimicrobial activity of essential oils of Salvia L. species. Ciência e Tecnologia de Alimentos, 29(4), 764-770.
• Porres-Martínez, M., González-Burgos, E., Carretero, M. E. & Gómez-Serranillos, M. P. (2013). Major selected monoterpenes α-pinene and 1,8-cineole found in Salvia lavandulifolia (Spanish sage) essential oil as regulators of cellular redox balance. Pharmaceutical Biology, 53(6), 921-929.
• Tzakou, O., Pitarokili, D., Chinou, I.B. & Harvala, C. (2001). Composition and antimicrobial activity of the essential oil of Salvia ringens. Planta Medica, 67, 81-83.
• Usano-Alemany, J., Herraiz-Penalver, D., Cuadrado, J., Diaz, S., Santa-Cruz, M. & Pala-Paul, J. (2012). Seasonal variation of the essential oils of Salvia lavandulifolia: Antibacterial activity. Journal of Essential Oil Bearing Plants, 15(2), 195-203.