Antibacterial Effect of Nanoparticles, Plant Activator and Some Chemicals on Suppression of Bacterial Cancer and Wilt Disease in Tomatoes

Year 2024, , 1526 - 1539, 16.09.2024

Omar Hatif Abdulrazzaq Benian Pınar Aktepe , Yesim Aysan

https://doi.org/10.47495/okufbed.1398842

Abstract

Clavibacter michiganensis subsp. michiganensis (Cmm), causal agent of Bacterial Canker and Wilt Disease are the important pathogens in tomatoes. In this study, the effects of two different nanoparticles [nano silver (AgNps) and nano copper (CuNps)], a plant activator (ISR-2000), copper hydroxide (a commercial fungicide, Kocide 2000) and two new chemical compounds [sodyum pentaborate pentahidrat (SPP), disodyum octaborate tetrahidrat (DOT)] were investigated on Suppression of Bacterial Canker and Wilt diseases in tomatoes. Except for nanomaterials (AgNps and CuNps), four other applications were successful in suppressing the disease. ISR-2000 and copper hydroxide applications were determined as the most successful applications by suppressing Bacterial Wilt Disease in tomato plants by 78% and 46%, respectively, and were in the same statistical group. SPP and DOT were the other successful treatments that suppressed disease emergence by 28% and 33%, but showed phytotoxic effects on tomato leaves. For this reason, chemicals containing SPP and DOT can be added to the active ingredients of pesticides to increase the effect of pesticides, but more detailed research is needed. While nanoparticles (AgNps and CuNps) gave promising antibacterial results in in vitro trials, they could not show the same effect in in vivo trials. Nanoparticles were evaluated as unsuccessful because they were statistically in the same group as the positive control. These results show that plant activators are the most successful application in suppressing bacterial infection. These applications are of great importance as alternative, environmentally friendly applications, especially against pathogens that have developed resistance to chemicals.

Keywords

Nanoparticular, Bacterial Wilt, SPP, DOT

Project Number

FDK-2022-14795

Nanopartiküller, Bitki Aktivatörü ve Bazı Kimyasalların Domates Bitkilerinde Bakteriyel Kanser ve Solgunluk Hastalığının Baskılanması Üzerine Antibakteriyel Etkisi

Abstract

Bakteriyel kanser ve solgunluk hastalığına neden olan Clavibacter michiganensis subsp. michiganensis (Cmm) domateste önemli bir patojendir. Bu çalışmada iki farklı metal nanopartikül [nano gümüş (AgNps) ve nano bakır (CuNps)], bitki aktivatörü (ISR-2000), bakır hidroksit ve iki yeni kimyasal bileşiğin [sodyum pentaborate pentahidrat (SPP), disodyum octaborate tetrahidrat (DOT)] hastalık üzerine olan baskılayıcı etkisi araştırılmıştır. Nanopartiküller (AgNps ve CuNps) hariç diğer dört uygulama hastalık çıkışını in vivo koşullarda baskılamada başarı göstermiştir. ISR-2000 ve bakır hidroksit uygulamaları domates bitkisinde hastalık çıkışını sırasıyla %78 ve %46 oranında baskılayan en başarılı uygulamalar olarak belirlenmiştir. SPP ve DOT uygulamaları hastalık çıkışını %28 ve %33 oranlarında baskılayan diğer başarılı uygulamalar olmakla birlikte domates yapraklarında fitotoksik etki göstermiştir. Bu nedenle SPP ve DOT içerikli kimyasallar, pestisitlerin aktif maddelerine eklenerek pestisitlerin etkisi artırılabilir ancak daha detaylı araştırmalara ihtiyaç olduğu açıktır. Nanopartiküller (AgNps ve CuNps) in vitro denemelerde umut verici antibakteriyel sonuçlar verirken, in vivo denemelerde aynı etkiyi gösterememiştir. Bu sonuçlar bitki aktivatörlerinin bakteriyel enfeksiyonu baskılamada en başarılı uygulama olduğunu göstermektedir. Bu uygulamalar özellikle kimyasallara direnç geliştirmiş patojenlere karşı alternatif, çevreye dost uygulamalar olarak büyük önem taşımaktadır.

Keywords

Nanopartikül, Bakteriyel Solgunluk, SPP, DOT

Supporting Institution

Çukurova Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

FDK-2022-14795

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