4,4'-Diaminodifenil Sülfür Bazlı Imin Bileşiğinin Spektral ve DNA Bağlama Özellikleri

Year 2023, Volume: 6 Issue: 1, 818 - 831, 10.03.2023

Mustafa Bal , Ayşegül Köse

https://doi.org/10.47495/okufbed.1180912

Abstract

Bu araştırmada, antikanser ilaçların temelini oluşturan ve iyi derecede biyolojik aktiviteye sahip olduğu bilinen, yeni bir 4,4'-Diaminodifenil sülfit bazlı Schiff bazı bileşiği [6,6'-((1E,1'E)-((thiobis (4,1-phenylene)) bis(azaneylylidene)) bis(methaneylylidene)) bis(3-(diethylamino) phenol) (A)] başarıyla sentezlenmiştir. Sentezlenen Schiff bazı bileşiğinin yapısal karekterizasyonu, FT-IR ve 1H-13C NMR, spektroskopileri ile belirlenmiştir. Ayrıca, bileşiğin moleküler yapısı, tek kristalli bir X-ışını kırınım çalışmasıyla belirlenmiştir. Bileşiğin DNA bağlama yeteneği, UV-vis spektroskopisi kullanılarak ölçülmüştür. Spektral değişimlerden faydalanılarak bileşiğe ait DNA bağlama sabiti Kb (M-1)= 6.25x105 olarak hesaplanmıştır. Bulunan Kb değeri interkalatif bir etkişimin varlığını önermektedir.

Keywords

Schiff Bases, DNA binding, X-ray diffraction, Drugs, Schiff Bases, DNA binding, X-ray diffraction, Drugs

Crystal Structure and DNA Binding Properties of A Sulfide Bridged Dimeric Schiff Base Compound

Abstract

In this research, a novel 4,4'-Diaminodiphenyl sulfide-based Schiff base compound [6,6'-((1E,1'E)-((thiobis (4,1-phenylene)) bis(azaneylylidene)) bis(methaneylylidene)) bis(3-(diethylamino) phenol) (A)], which is known to have good biological activity and forms the basis of anticancer drugs, was successfully synthesized. Structural characterization of the synthesized Schiff base compound was determined by FT-IR and 1H-13C NMR, spectroscopies. Also, the molecular structure of the compound was determined by a single-crystal X-ray diffraction study. The DNA binding ability of the compound was measured using UV-vis spectroscopy. Using the spectral changes, the DNA binding constant of the compound was calculated as Kb (M-1)= 6.25x105. The Kb value found suggests the existence of an intercalative interaction.

Keywords

Schiff Bases, DNA binding, X-ray diffraction, Drugs, Schiff Bases, DNA binding, X-ray diffraction, Drugs

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