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OLGUNLAŞMA SÜRECİNDE ALTI KAYISI ÇEŞİDİNİN ENZİM PARAMETRELERİNDEKİ DEĞİŞİMLER

Yıl 2014, Cilt: 29 Sayı: 3, 174 - 178, 30.10.2014
https://doi.org/10.7161/anajas.2014.29.3.174-178

Öz

Bu çalışmada, Malatya bölgesinde yetiştirilen farklı kayısı çeşitlerinin olgunlaşma periyodu boyunca bazı enzimatik ve fiziksel değişimleri incelenmiştir. Ham, yarı olgun ve olgun dönemde hasat edilmiş olan altı kayısı çeşidinin (‘Hasanbey’, ‘Canino’, ‘Turfanda Eskimalatya’, ‘Hacihaliloglu’, ‘Ozal’ ve ‘Levent’) meyvelerinde meyve eti sertliği, poligalakturonaz ve pektin metilesteraz enzim aktiviteleri belirlenmiştir. Poligalakturonaz ve pektin metilesteraz enzim aktiviteleri meyve gelişimi boyunca artarken, meyve eti sertliği azalmıştır. En yüksek enzim aktiviteleri ‘Levent’ kayısı çeşidinde saptanmıştır.

Kaynakça

  • Arancibia, R.A., Motsenbocker, C.E. 2004. Pectin ultra-degradation decreases the force required to detach ripe fruit from the calyx in tabasco pepper. J Amer. Soc. Hort. Sci., 129: 642-648.
  • Asma , B.M. 2000. Apricot Cultivation, Malatya, Turkey, pp.12.
  • Belleau, C.D., Vallet, A., Done`che, B., Geny, L. 2008. Pectin methylesterase and polygalacturonase in the developing grape skin. Plant Physiol. Biochem., 46: 638-646.
  • Bradford, M.M. 1976. A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
  • Brummel, D.A., Harpster, M.H. 2001. Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol. Biol., 47: 311-340.
  • Buttner, R. 2001. Armeniaca. In: P. Hanelt. Institute of Plant Genetics and Crop Plant Researches (eds.). Mansfelds Encyclopedia of Agricultural and Horticultural Crops pp.523-527.
  • Deng, Y., Wu, Y., Li, Y. 2005. Changes in firmness, cell wall composition and cell wall hydrolases of grapes stored in high oxygen atmospheres. Food Res. Int., 38: 769-776.
  • Duncan, D.B. 1955. Multiple range and multiple F Tests. Biometrics, 11: 1-14.
  • Ercisli, S. 2004. A short review of the fruit germplasm resources of Turkey. Genet. Res. Crop. Evol., 51, 419-435.
  • Faust, M., Suranyi, D., Nyujto, F. 1998. Origin and Dissemination of Apricot. Horticultural reviews, John Wiley and Sons, Inc. 22: 225-266.
  • Figueroa, C.R., Rosli, H.G., Civello, P.M., Martinez, G.A., Herrera, R., Moya-Leon, M.A. 2010. Changes in cell wall polysaccarides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria x ananassa fruits. Sci. Hortic., 124: 454-462.
  • Giovannoni, J. 2001. Molecular regulation of fruit ripening. Ann. Rev. Plant Physio. Plant Mol. Biol., 52: 725-749.
  • Hagerman, A.E., Austin, P.J. 1986. Continuous spectrophotometric assay for plant pectin methyl esterase. J Agr. Food Chem., 34: 440-444.
  • Hegedus, A., Engel, R., Abrankol, L., Balogh, E., Blazovics, A., Herman, R., Halasz, J., Ercisli, S., Pedryc, A., Stefanovitsbanyai, S. 2010. Antioxidant and antiradical capacities in apricot (Prunus armeniaca L.) fruits: Variations from genotypes, years, and analytical methods. J Food Sci., 75: 722-730.
  • Hubbard, N.L., Pharr, O.M. 1992. Developmental changes in carbohydrate concentration and activities of sucrose metabolizing enzymes in fruits of two Capsicum annuum L. genotypes. Plant Sci., 86: 33-39.
  • Jain, N., Dhawan, K., Malhotra, S., Singh R. 2003. Biochemistry of Fruit Ripening of Guava (Psidium guajava L.): Compositional and Enzymatic Changes. Plant Food Hum. Nutr., 58: 309-315.
  • Jayani, R. S., Saxena, S., Gupta, R. 2005. Microbial pectinolytic enzymes: A review. Process Biochem., 40: 2931-2944.
  • Jiang, Y. M., Li, Y. B. 2000. Effects of chitosan coating on postharvest life and quality of longan fruit, Food Chem., 73: 139-143.
  • Li, J., Zhang, P. P., Chen, J. Z., Yao, Q., Jiang, Y. M. 2009. Cellular wall metabolism in citrus fruit pericarp and its relation to creasing fruit rate. Sci. Hortic., 122: 45-50.
  • Lohani, S., Trivedi, P.K., Nath, P. 2004. Changes in activities of cell wall hydrolases during ethylene induced ripening in banana: effect of 1-MCP, ABA and IAA. Postharvest Biol. Tec., 31: 119-126.
  • Manoj, K M., Lakner, F.J., Hager, L.P. 2000. Epoxidation of indene by chloroperoxidase. J Mol. Catal. B Enzym., 9: 107-111.
  • Nelson, N. 1944. A photometric adaption of the Somogyi method for the determination of glucose. J Biol. Chem., 153: 375-380.
  • Nunes, C., Santos, C., Pinto, G., Silva, S., Lopes, da Silva J. A., Saraiva, J.A., Coimbra, M.A. 2009. Effects of ripening on microstructure and texture of ‘Ameixa d’Elvas’ candied plums. Food Chem., 115: 1094-1101.
  • Payasi, A., Misra, P.C., Sanwal, G.G. 2004. Effect of phytohormones on pectate lyase activity in ripening Musa acuminata. Plant Physiol. Bioch., 42: 861-865.
  • Pelayo, C., Vilas-Boas, E., Benichou, M., Kader, A.A. 2002. Variability in response of partially ripe bananas to 1-methylcyclopropene. Postharvest Biol. Technol., 28: 75-85.
  • Pinzon, I.M., Fischer, G., Corredor, G. 2007. Determinacion de los estados de madurez del fruto de gulupa (Passiflora edulis Sims.). Agron. Colomb., 25: 83-95.
  • Prasanna, V., Prabha, T. N., Tharanathan, R.N. 2005. Multiple forms of polygalacturonase from mango fruit. Food Chem., 95: 30-36.
  • Prinsi, B., Negri, A. S., Fedeli, C., Morgutti, S., Negrini, N., Cocucci, M., Espen, L. 2011. Peach fruit ripening: A proteomic comparative analysis of the mesocarp of two cultivars with different flesh firmness at two ripening stages. Phytochem., 72: 1251-1262.
  • Somogyi, M. 1952. Notes on sugar determination. J Biol. Chem., 195: 19-23.
  • Sturm, K., Koron, D., Stampar, F. 2003. The composition of fruit of different strawberry varieties depending on maturity stage. Food Chem., 83: 417-422.
  • Usenik, V., Fabèiè, J., Štampar, F. 2008. Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem.. 107: 185-192.
  • Villarreal, N.M., Rosli, H.G., Martinez, G.A., Civello, P.M. 2008. Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness. Postharvest Biol. Tec., 47: 141-150.
  • Yentur, S. 1995. Plant Anatomy, Istanbul University Publication, İstanbul, Turkey, 492-512 p.
  • Zhou, H.W., Arie, R. B., Lurie, S. 2000. Pectin esterase, polygalacturonase and gel formation in peach pectin fractions. Phytochem., 55: 191-195

CHANGES IN SOME ENZYMATIC PARAMETERS OF SIX APRICOT CULTIVARS DURING RIPENING

Yıl 2014, Cilt: 29 Sayı: 3, 174 - 178, 30.10.2014
https://doi.org/10.7161/anajas.2014.29.3.174-178

Öz

In this study, some enzymatic and physical changes were investigated during different ripening periods in fruits of some apricot cultivars grown in Malatya province, Turkey. Firmness, polygalacturonase and pectin methylesterase enzyme activities were monitored in fruits of six apricot cultivars (‘Hasanbey’, ‘Canino’, ‘Turfanda Eskimalatya’, ‘Hacihaliloglu’, ‘Ozal’ and ‘Levent’) harvested at unripe (hard green), half ripe (mature green) and ripe stages. The polygalacturonase and pectin methylesterase enzyme activities increased during fruit development while fruit flesh firmness decreased. The highest enzyme activities were found in ‘Levent’ cultivar.

Kaynakça

  • Arancibia, R.A., Motsenbocker, C.E. 2004. Pectin ultra-degradation decreases the force required to detach ripe fruit from the calyx in tabasco pepper. J Amer. Soc. Hort. Sci., 129: 642-648.
  • Asma , B.M. 2000. Apricot Cultivation, Malatya, Turkey, pp.12.
  • Belleau, C.D., Vallet, A., Done`che, B., Geny, L. 2008. Pectin methylesterase and polygalacturonase in the developing grape skin. Plant Physiol. Biochem., 46: 638-646.
  • Bradford, M.M. 1976. A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
  • Brummel, D.A., Harpster, M.H. 2001. Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol. Biol., 47: 311-340.
  • Buttner, R. 2001. Armeniaca. In: P. Hanelt. Institute of Plant Genetics and Crop Plant Researches (eds.). Mansfelds Encyclopedia of Agricultural and Horticultural Crops pp.523-527.
  • Deng, Y., Wu, Y., Li, Y. 2005. Changes in firmness, cell wall composition and cell wall hydrolases of grapes stored in high oxygen atmospheres. Food Res. Int., 38: 769-776.
  • Duncan, D.B. 1955. Multiple range and multiple F Tests. Biometrics, 11: 1-14.
  • Ercisli, S. 2004. A short review of the fruit germplasm resources of Turkey. Genet. Res. Crop. Evol., 51, 419-435.
  • Faust, M., Suranyi, D., Nyujto, F. 1998. Origin and Dissemination of Apricot. Horticultural reviews, John Wiley and Sons, Inc. 22: 225-266.
  • Figueroa, C.R., Rosli, H.G., Civello, P.M., Martinez, G.A., Herrera, R., Moya-Leon, M.A. 2010. Changes in cell wall polysaccarides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria x ananassa fruits. Sci. Hortic., 124: 454-462.
  • Giovannoni, J. 2001. Molecular regulation of fruit ripening. Ann. Rev. Plant Physio. Plant Mol. Biol., 52: 725-749.
  • Hagerman, A.E., Austin, P.J. 1986. Continuous spectrophotometric assay for plant pectin methyl esterase. J Agr. Food Chem., 34: 440-444.
  • Hegedus, A., Engel, R., Abrankol, L., Balogh, E., Blazovics, A., Herman, R., Halasz, J., Ercisli, S., Pedryc, A., Stefanovitsbanyai, S. 2010. Antioxidant and antiradical capacities in apricot (Prunus armeniaca L.) fruits: Variations from genotypes, years, and analytical methods. J Food Sci., 75: 722-730.
  • Hubbard, N.L., Pharr, O.M. 1992. Developmental changes in carbohydrate concentration and activities of sucrose metabolizing enzymes in fruits of two Capsicum annuum L. genotypes. Plant Sci., 86: 33-39.
  • Jain, N., Dhawan, K., Malhotra, S., Singh R. 2003. Biochemistry of Fruit Ripening of Guava (Psidium guajava L.): Compositional and Enzymatic Changes. Plant Food Hum. Nutr., 58: 309-315.
  • Jayani, R. S., Saxena, S., Gupta, R. 2005. Microbial pectinolytic enzymes: A review. Process Biochem., 40: 2931-2944.
  • Jiang, Y. M., Li, Y. B. 2000. Effects of chitosan coating on postharvest life and quality of longan fruit, Food Chem., 73: 139-143.
  • Li, J., Zhang, P. P., Chen, J. Z., Yao, Q., Jiang, Y. M. 2009. Cellular wall metabolism in citrus fruit pericarp and its relation to creasing fruit rate. Sci. Hortic., 122: 45-50.
  • Lohani, S., Trivedi, P.K., Nath, P. 2004. Changes in activities of cell wall hydrolases during ethylene induced ripening in banana: effect of 1-MCP, ABA and IAA. Postharvest Biol. Tec., 31: 119-126.
  • Manoj, K M., Lakner, F.J., Hager, L.P. 2000. Epoxidation of indene by chloroperoxidase. J Mol. Catal. B Enzym., 9: 107-111.
  • Nelson, N. 1944. A photometric adaption of the Somogyi method for the determination of glucose. J Biol. Chem., 153: 375-380.
  • Nunes, C., Santos, C., Pinto, G., Silva, S., Lopes, da Silva J. A., Saraiva, J.A., Coimbra, M.A. 2009. Effects of ripening on microstructure and texture of ‘Ameixa d’Elvas’ candied plums. Food Chem., 115: 1094-1101.
  • Payasi, A., Misra, P.C., Sanwal, G.G. 2004. Effect of phytohormones on pectate lyase activity in ripening Musa acuminata. Plant Physiol. Bioch., 42: 861-865.
  • Pelayo, C., Vilas-Boas, E., Benichou, M., Kader, A.A. 2002. Variability in response of partially ripe bananas to 1-methylcyclopropene. Postharvest Biol. Technol., 28: 75-85.
  • Pinzon, I.M., Fischer, G., Corredor, G. 2007. Determinacion de los estados de madurez del fruto de gulupa (Passiflora edulis Sims.). Agron. Colomb., 25: 83-95.
  • Prasanna, V., Prabha, T. N., Tharanathan, R.N. 2005. Multiple forms of polygalacturonase from mango fruit. Food Chem., 95: 30-36.
  • Prinsi, B., Negri, A. S., Fedeli, C., Morgutti, S., Negrini, N., Cocucci, M., Espen, L. 2011. Peach fruit ripening: A proteomic comparative analysis of the mesocarp of two cultivars with different flesh firmness at two ripening stages. Phytochem., 72: 1251-1262.
  • Somogyi, M. 1952. Notes on sugar determination. J Biol. Chem., 195: 19-23.
  • Sturm, K., Koron, D., Stampar, F. 2003. The composition of fruit of different strawberry varieties depending on maturity stage. Food Chem., 83: 417-422.
  • Usenik, V., Fabèiè, J., Štampar, F. 2008. Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem.. 107: 185-192.
  • Villarreal, N.M., Rosli, H.G., Martinez, G.A., Civello, P.M. 2008. Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness. Postharvest Biol. Tec., 47: 141-150.
  • Yentur, S. 1995. Plant Anatomy, Istanbul University Publication, İstanbul, Turkey, 492-512 p.
  • Zhou, H.W., Arie, R. B., Lurie, S. 2000. Pectin esterase, polygalacturonase and gel formation in peach pectin fractions. Phytochem., 55: 191-195
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Bahçe Bitkileri
Yazarlar

Zehra Abacı Bu kişi benim

Bayram Asma

Yayımlanma Tarihi 30 Ekim 2014
Yayımlandığı Sayı Yıl 2014 Cilt: 29 Sayı: 3

Kaynak Göster

APA Abacı, Z., & Asma, B. (2014). CHANGES IN SOME ENZYMATIC PARAMETERS OF SIX APRICOT CULTIVARS DURING RIPENING. Anadolu Tarım Bilimleri Dergisi, 29(3), 174-178. https://doi.org/10.7161/anajas.2014.29.3.174-178
Online ISSN: 1308-8769