Yerel ceviz çeşidinde (Juglans regia L.) abiyotik stres faktörlerine karşı dayanıklılık mekanizmasının belirlenmesi

Yıl 2016, Cilt: 31 Sayı: 3, 321 - 331, 23.10.2016

Nezahat Turfan

https://doi.org/10.7161/omuanajas.269984

Cited By: 1

Öz

Bu çalışmada yerel ceviz (Juglans regia) genotipinin farklı abiyotik stres faktörlerine karşı tepkisi araştırılmıştır. Bunun için 5 L hacminde 2:2:1 oranında harç (torf:bahçe toprağı:kum) karışımı içeren saksılarda yetiştirilen 5-6 yapraklı ceviz fidanlarına tuz (75, 150 ve 225 mM NaCl), ağır metal (2 mg/L FeCl3, NiCl2 ve ZnCl2), kireç (2 g/L CaCO3), fabrika baca tozu (2 g/L) ve kurak (%50) stresi uygulamaları altı hafta süresince uygulanmıştır. Uygulamalardan sonra toplanan yaprak örneklerinde fotosentetik pigment, protein, prolin miktarı, malondialdehit (MDA) ve hidrojen peroksit (H2O2) konsantrasyonu, APx, CAT ve SOD aktiviteleri ölçülmüştür. Bulgulara göre klorofil b, toplam klorofil ve karotenoit miktarı kireç ve ağır metal uygulamasında; protein demir, baca tozu ve çinko uygulamasında; prolin, H2O2, APx ve SOD değerleri tüm stres uygulamalarında; CAT değeri çinko, nikel ve kireç uygulamalarında yüksek bulunmuştur. MDA içeriği ise kireç uygulamasında yüksek, diğer gruplarda düşüktür. Sonuç olarak yerel ceviz genotipi, tuz konsantrasyonları ve kuraklığa duyarlı; ağır metal toksisitesine toleranslı ve fabrika baca tozu ve CaCO3 uygulamalarına ise orta derecede toleranslı bulunmuştur.

Anahtar Kelimeler

Abiyotik stres, ceviz; dayanıklık; Juglans regia

Determining of resistance mechanism against abiotic stress factories in native walnut variety (Juglans regia L.)

Öz

This study investigated the reactions of native walnut genotype (Juglans regia L.) against different abiotic stress factors. For this purpose, 5-6 leaves seedlings grown in the plastic pots having the mixture of peat, garden soil and sand (2:2:1) were treated with salinity (75, 150 and 225 mM NaCl), heavy metals (2 mg/L FeCl3, NiCl2 and ZnCl2), lime (2 g/L CaCO3), pollution (2 g/L factory flue dust) and drought (50%) for six weeks. After the treatments, photosynthetic pigment, proline, total soluble protein, peroxidation level (MDA-malondialdehyde), H2O2, APx, CAT and SOD activities in the leaf samples were analysed. Results showed that chlorophyll b and total chlorophyll and carotenoids were highest for the lime and heavy metals treatments, whereas total soluble protein was highest for iron, zinc and factory flue dust treatments. CAT activity was highest for zinc, nickel and lime treatments, while proline, H2O2 concentration, APx and SOD activity were highest for all treatments. Lipid peroxidation level (malondialdehyde-MDA) starch showed an increase only with the lime treated leaves but it was lower with other treatments. In conclusion, the results indicate that the studied walnut genotype is much tolerant for heavy metal toxicity while it is susceptible to salinity concentrations and drought. In addition, walnut variety shows medium tolerance against the factory flue dust and lime.

Anahtar Kelimeler

Abiotic stress, walnut; resistance; Juglans regia

Kaynakça

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