Genetic diversity of pepper genotypes as assessed by SRAP markers

Yıl 2017, Cilt: 32 Sayı: 3, 321 - 329, 15.10.2017

M.Kadri Bozkalfa , Tansel Kaygısız Aşcıoğlu , Dursun Eşiyok

https://doi.org/10.7161/omuanajas.284511

Cited By: 5

Öz

Turkey’s geographical position played an
important role in spread of pepper cultivation in neighbouring countries, and a
large number of cultivars and landraces are grown in commercial production in
Turkey. The performance of pepper breeding depends on discovery of new sources
of genetic variation, identification of accessions with useful traits, and their
combination with desired agronomic properties. Characterization and evaluation
are the first steps in the development of new cultivars. This research was carried
to asses molecular characterization of 94 local pepper accessions using with
SRAP (sequence related amplified polymorphism) marker system. The plant
materials examined in the present research were obtained from pepper landraces
evaluated for agro-morfological characterizations during the 2004-2007
experiment years. Molecular characterization informed that examined plant
collection observed high genetic diversity, and most of the accessions showed
low genetic similarities placed further distance on dendogram. Molecular traits
recorded with 33 SRAP combinations on all pepper genotypes. The result of
molecular traits 85% of total variance has taken place in 9 factor groups and
genetically differences were changed between 62-94% among pepper genotypes. Present
results highlighted that characterized plant pepper collection will reduce
breeding duration and results informed valuable contribution for further pepper
breeding programmes.

Anahtar Kelimeler

Breeding, Molecular characterization, Pepper, SRAP markers, Variability

Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi

Öz

Türkiyenin coğrafik konumu itibariyle ticaret
yolları üzerinde yer alması biberin diğer ülkelere yayılmasında etkili olmuştur.
Ülkemizde çok sayıda yerel populasyonlara ve ticari çeşitler biber
yetiştiriciliğinde kullanılmaktadır. Biber ıslahının başarısı yüksek genetik
çeşitliliğe sahip kaynakların varlığına, genetik materyalin özelliklerine ve
istenen özelliğe sahip kombinasyonların oluşturulmasına dayanmaktadır. Bitkisel
materyalin karekterizasyonu bu işlemin ilk adımını oluşturmaktadır. Bu
çalışmada kullanılan bitkisel materyalin kaynağını; 2004-2007 yılları arasında
yürütülen, yerel biber genotiplerinin morfolojik ve agronomik özellikler
yönünden karakterizasyonu amacıyla yürütülen çalışmadan sağlanan gen kaynağını
oluşturmaktadır. Morfolojik özellikleri tanımlanan bu gen havuzunun moleküler
yöntemler ile karakterizasyonu amacıyla yürütülen bu projede ise toplam 94
yerel biber genotipinin birbirlerine olan genetik uzaklıkları SRAP (Sequence related amplified polymorphism)
markör sistemi kullanılarak belirlenmiştir. Elde edilen veriler istatistiki
olarak değerlendirildiğinde incelenen genotiplerin yüksek düzeyde varyabilitiye
sahip, büyük bir bölümünün genetik yönden uzak akraba olduğu ve dendogramda
farklı gruplarda yer aldığı görülmüştür. 33 SRAP markör kombinasyonu
kullanılarak yapılan moleküler analizler sonucunda varyasyonun %85’i 9 faktör
grubunda yer almış ve genotiplerin genetik uzaklıkları %62 ile %94 arasında
değişmiştir. Bu sonuçlar ileride yürütülecek ıslah programlarında genotiplerin
daha etkin kullanımına imkan sağlarken ıslah süresini büyük ölçüde
kısaltacaktır. 

Anahtar Kelimeler

Biber, ıslah, moleküler karakterizasyon, SRAP markörleri

Kaynakça

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