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Genetic diversity of pepper genotypes as assessed by SRAP markers

Yıl 2017, Cilt: 32 Sayı: 3, 321 - 329, 15.10.2017
https://doi.org/10.7161/omuanajas.284511

Öz

Turkey’s geographical position played an
important role in spread of pepper cultivation in neighbouring countries, and a
large number of cultivars and landraces are grown in commercial production in
Turkey. The performance of pepper breeding depends on discovery of new sources
of genetic variation, identification of accessions with useful traits, and their
combination with desired agronomic properties. Characterization and evaluation
are the first steps in the development of new cultivars. This research was carried
to asses molecular characterization of 94 local pepper accessions using with
SRAP (sequence related amplified polymorphism) marker system. The plant
materials examined in the present research were obtained from pepper landraces
evaluated for agro-morfological characterizations during the 2004-2007
experiment years. Molecular characterization informed that examined plant
collection observed high genetic diversity, and most of the accessions showed
low genetic similarities placed further distance on dendogram. Molecular traits
recorded with 33 SRAP combinations on all pepper genotypes. The result of
molecular traits 85% of total variance has taken place in 9 factor groups and
genetically differences were changed between 62-94% among pepper genotypes. Present
results highlighted that characterized plant pepper collection will reduce
breeding duration and results informed valuable contribution for further pepper
breeding programmes.

Kaynakça

  • Andrews, J. 1999. The pepper trail: history and recipes from around the world. Univ. North Texas Press, Denton, Tex.
  • Aktaş, H., Abak, K., Şensoy, S. 2009: Genetic diversity in some Turkish pepper (Capsicum annum L.) genotypes revealed by AFLP analysis. Afr. J. Biotechnol. 8(18):.4378-7386.
  • Apuya, N.R., Frazier, B.L., Keim, P., Jillroth, E., Lark, K.G. 1988. Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) Merill. Theor. Appl. Genet. 75: 889–901.
  • Bozokalfa, M.K., Eşiyok, D. 2011. Evaluation of morphological and agronomical characterization of Turkish pepper accessions. Int. J. Veg. Sci. 17: 115-135.
  • Bozokalfa, M.K., Eşiyok, D., Turhan, K. 2009. Patterns of phenotypic variation in a germplasm collection of pepper (Capsicum annuum L.) from Turkey. Span. J. Agric. Res. 7: 83-95.
  • Budak, H., Shearman, R.C., Parmaksiz I., Gaussoin, R.E., Riordan T.P., Dweikat I. 2004. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theor. Appl. Genet. 108: 328-334.
  • Dias, J.S., Lima, M.B., Song, K.M., Monterio, A.A., Williams, P.H., Osborn, T.C. 1992. Molecular taxonomy of portuguese tronchuda cabbage and kale landraces using nuclear RFLPs. Euphytica 58: 221–229.
  • Dias, J.S., Monterio, A.A., Lima, M.B. 1993. Numerical taxonomy of portuguese tronchuda cabbage and galega kale landraces using morphological characters. Euphytica 69: 51–68.
  • Doyle, J.J., Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
  • Ender, M., Terpstra K., Kelly, J.D. 2008. Marker-assisted selection for white mold resistance in common bean. Mol. Breed. 21: 149-157.
  • Eşiyok, D. 2012. Kışlık ve yazlık sebze yetiştiriciliği. Meta Basım, 404 s. Bornova/İzmir.
  • Ferriol, M., Pico, B., Nuez, F. 2003. Genetic diversity of a germplasm collection of Cucurbita pepo Using SRAP and AFLP Markers. Theor. Appl. Genet. 107: 271–282.
  • Figdore, S.S., Kennard, W.C., Song, K.M., Slocum, M.K., Osborn, T.C. 1988. Assessment of the degree of restriction length polymorphism in Brassica. Theor. Appl. Genet. 75:833–940.
  • Frary, A, Kegeli, M.A, Okmen B, Sigva H.O, Yemenicioglu, A., Doganlar, S. 2008. Water-soluble antioxidant potential of Turkish pepper cultivars. J. Am. Soc. Hortic. Sci. 43: 631-636
  • Geleta, L.F., Labuschagne, M.T., Viljoen, C.D. 2005. Genetic variability in pepper (Capsicum annuum L.) estimated by morphological data and amplified fragment lenth polymorphism markers. Biodivers. Conserv 14: 2361-2375.
  • Gepts, P., 1995. Genetic markers and core collections. ın core collections of plant genetic resources (Eds T. Hodgkin, A. H. D. Brown, Th. J. L. van Hintum & E. A. V. Morales), pp. 127–146. Chichester, UK: John Wiley & Sons.
  • Grisi, M.C., Blair, M.W., Gepts, P., Brondani, C., Pereira, P.A., Brondani, R.P. 2007. Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 x Jalo EEP558. Gen. Mol. Res. 30: 691-706.
  • Gulsen, O., Shearman, R.C., Heng-Moss, T.M., Mutlu, N., Lee, D.J., Sarath, G. 2007. Peroxidase gene polymorphism in buffalograss and other grasses. Crop Sci. 47: 767-772.
  • Haussmann, B.I.G, Parzies, H.K. Presterl T, Susic, Z., Miedaner, T. 2004 Plant genetic resources in crop improvement. Plant Genet Resour-C 2: 3–21.
  • Keleş, D., Özgen, Ş., Saraçoğlu, O., Ata, A., Özgen, M. 2016. Antioksidant potential of turkish pepper (Capsium annuum L.) genotypes at two different maturity stage. Turk. J. Agric. For. 40: 542-551.
  • Koutita, O., Tertivanidis, K., Koutsos, T.V., Koutsika-Sotiriou, M., Skaracisi, G.N..2005. Genetic diversity in four cabbage populations based on random amplified polymorphic DNA markers. J. Agric. Sci. 143: 377–384.
  • Lefebvre, V., Palloix, A., Rives, M. 1993. Nuclear RFLP between pepper cultivars (Capsicum annuum L.). Euphytica 71: 189–199.
  • Li, G., Quiros, C.F. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in brassica. Theor. Appl. Genet. 103: 455–461.
  • Long, Y., Shi, J., Qiu, D., Li, R., Zhang, C., Wang, J., Hou, J., Zhao, J., Shi, L., Beom-Seok, P., Choi, S.R., Lim, Y.P., Meng, J. 2007. Flowering time quantitative trait loci analysis of oilseed brassica in multiple environments and genomewide alignment with arabidopsis. Genetics 17(4): 2433-2444.
  • Miller, J.C., Tanksley, S.D. 1989. RFLP analysis of polygenetic relationships and genetic variation in the genus lycopersicon. Theor. Appl. Genet. 80: 437–448.
  • Mohammadi, S.A. Prassanna, B.M. 2003. Analysis of genetic diversity in crop plants-salient statistical tools and considerations, Crop Sci. 43: 1235–1248.
  • Nsabiyera, V., Logose, M., Ochwo-Ssemakula, M., Sseruwagi, P., Gibson, P., Ojiewo, C. 2013. Morphological characterization of local and exotic hot pepper (Capsicum annuum L.) collections in Uganda. Biorem. Biodiv Bioavail 7(1): 22-32.
  • Papa, R., Gepts, P. 2003. Asymmetry of gene flow and differential geographical structure of molecular diversity in wild and domesticated common bean (Phaseolus vulgaris L.) from mesoamerica. Theor. Appl. Genet. 106(2): 239-250.
  • Smith, J.S.C., Smith, O.S. 1989. The description and assessment of distances between ınbred lines of maize: the utility of morphological, biochemical and genetic descriptors and a scheme for the testing of distinctiveness between ınbred lines. Maydica 34: 151-161.
  • Souza, E., Sorrells, M.E. 1991. Genetic relationships among 70 north american oat cultivars, ı: cluster analysis using quantitative morphological characters. Crop Sci. 31: 599-605.
  • Toquica S. P., Rodriguez, F., Martinez, E., Duque, M.C., Tohme, J. 2003. Molecular characterization by aflps of capsicum germplasm from the amazon department in colombia, characterization by AFLPs of Capsicum. Genet. Resour. Crop Evol. 50: 639–647.

Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi

Yıl 2017, Cilt: 32 Sayı: 3, 321 - 329, 15.10.2017
https://doi.org/10.7161/omuanajas.284511

Öz

Türkiyenin coğrafik konumu itibariyle ticaret
yolları üzerinde yer alması biberin diğer ülkelere yayılmasında etkili olmuştur.
Ülkemizde çok sayıda yerel populasyonlara ve ticari çeşitler biber
yetiştiriciliğinde kullanılmaktadır. Biber ıslahının başarısı yüksek genetik
çeşitliliğe sahip kaynakların varlığına, genetik materyalin özelliklerine ve
istenen özelliğe sahip kombinasyonların oluşturulmasına dayanmaktadır. Bitkisel
materyalin karekterizasyonu bu işlemin ilk adımını oluşturmaktadır. Bu
çalışmada kullanılan bitkisel materyalin kaynağını; 2004-2007 yılları arasında
yürütülen, yerel biber genotiplerinin morfolojik ve agronomik özellikler
yönünden karakterizasyonu amacıyla yürütülen çalışmadan sağlanan gen kaynağını
oluşturmaktadır. Morfolojik özellikleri tanımlanan bu gen havuzunun moleküler
yöntemler ile karakterizasyonu amacıyla yürütülen bu projede ise toplam 94
yerel biber genotipinin birbirlerine olan genetik uzaklıkları SRAP (Sequence related amplified polymorphism)
markör sistemi kullanılarak belirlenmiştir. Elde edilen veriler istatistiki
olarak değerlendirildiğinde incelenen genotiplerin yüksek düzeyde varyabilitiye
sahip, büyük bir bölümünün genetik yönden uzak akraba olduğu ve dendogramda
farklı gruplarda yer aldığı görülmüştür. 33 SRAP markör kombinasyonu
kullanılarak yapılan moleküler analizler sonucunda varyasyonun %85’i 9 faktör
grubunda yer almış ve genotiplerin genetik uzaklıkları %62 ile %94 arasında
değişmiştir. Bu sonuçlar ileride yürütülecek ıslah programlarında genotiplerin
daha etkin kullanımına imkan sağlarken ıslah süresini büyük ölçüde
kısaltacaktır. 

Kaynakça

  • Andrews, J. 1999. The pepper trail: history and recipes from around the world. Univ. North Texas Press, Denton, Tex.
  • Aktaş, H., Abak, K., Şensoy, S. 2009: Genetic diversity in some Turkish pepper (Capsicum annum L.) genotypes revealed by AFLP analysis. Afr. J. Biotechnol. 8(18):.4378-7386.
  • Apuya, N.R., Frazier, B.L., Keim, P., Jillroth, E., Lark, K.G. 1988. Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) Merill. Theor. Appl. Genet. 75: 889–901.
  • Bozokalfa, M.K., Eşiyok, D. 2011. Evaluation of morphological and agronomical characterization of Turkish pepper accessions. Int. J. Veg. Sci. 17: 115-135.
  • Bozokalfa, M.K., Eşiyok, D., Turhan, K. 2009. Patterns of phenotypic variation in a germplasm collection of pepper (Capsicum annuum L.) from Turkey. Span. J. Agric. Res. 7: 83-95.
  • Budak, H., Shearman, R.C., Parmaksiz I., Gaussoin, R.E., Riordan T.P., Dweikat I. 2004. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theor. Appl. Genet. 108: 328-334.
  • Dias, J.S., Lima, M.B., Song, K.M., Monterio, A.A., Williams, P.H., Osborn, T.C. 1992. Molecular taxonomy of portuguese tronchuda cabbage and kale landraces using nuclear RFLPs. Euphytica 58: 221–229.
  • Dias, J.S., Monterio, A.A., Lima, M.B. 1993. Numerical taxonomy of portuguese tronchuda cabbage and galega kale landraces using morphological characters. Euphytica 69: 51–68.
  • Doyle, J.J., Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
  • Ender, M., Terpstra K., Kelly, J.D. 2008. Marker-assisted selection for white mold resistance in common bean. Mol. Breed. 21: 149-157.
  • Eşiyok, D. 2012. Kışlık ve yazlık sebze yetiştiriciliği. Meta Basım, 404 s. Bornova/İzmir.
  • Ferriol, M., Pico, B., Nuez, F. 2003. Genetic diversity of a germplasm collection of Cucurbita pepo Using SRAP and AFLP Markers. Theor. Appl. Genet. 107: 271–282.
  • Figdore, S.S., Kennard, W.C., Song, K.M., Slocum, M.K., Osborn, T.C. 1988. Assessment of the degree of restriction length polymorphism in Brassica. Theor. Appl. Genet. 75:833–940.
  • Frary, A, Kegeli, M.A, Okmen B, Sigva H.O, Yemenicioglu, A., Doganlar, S. 2008. Water-soluble antioxidant potential of Turkish pepper cultivars. J. Am. Soc. Hortic. Sci. 43: 631-636
  • Geleta, L.F., Labuschagne, M.T., Viljoen, C.D. 2005. Genetic variability in pepper (Capsicum annuum L.) estimated by morphological data and amplified fragment lenth polymorphism markers. Biodivers. Conserv 14: 2361-2375.
  • Gepts, P., 1995. Genetic markers and core collections. ın core collections of plant genetic resources (Eds T. Hodgkin, A. H. D. Brown, Th. J. L. van Hintum & E. A. V. Morales), pp. 127–146. Chichester, UK: John Wiley & Sons.
  • Grisi, M.C., Blair, M.W., Gepts, P., Brondani, C., Pereira, P.A., Brondani, R.P. 2007. Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 x Jalo EEP558. Gen. Mol. Res. 30: 691-706.
  • Gulsen, O., Shearman, R.C., Heng-Moss, T.M., Mutlu, N., Lee, D.J., Sarath, G. 2007. Peroxidase gene polymorphism in buffalograss and other grasses. Crop Sci. 47: 767-772.
  • Haussmann, B.I.G, Parzies, H.K. Presterl T, Susic, Z., Miedaner, T. 2004 Plant genetic resources in crop improvement. Plant Genet Resour-C 2: 3–21.
  • Keleş, D., Özgen, Ş., Saraçoğlu, O., Ata, A., Özgen, M. 2016. Antioksidant potential of turkish pepper (Capsium annuum L.) genotypes at two different maturity stage. Turk. J. Agric. For. 40: 542-551.
  • Koutita, O., Tertivanidis, K., Koutsos, T.V., Koutsika-Sotiriou, M., Skaracisi, G.N..2005. Genetic diversity in four cabbage populations based on random amplified polymorphic DNA markers. J. Agric. Sci. 143: 377–384.
  • Lefebvre, V., Palloix, A., Rives, M. 1993. Nuclear RFLP between pepper cultivars (Capsicum annuum L.). Euphytica 71: 189–199.
  • Li, G., Quiros, C.F. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in brassica. Theor. Appl. Genet. 103: 455–461.
  • Long, Y., Shi, J., Qiu, D., Li, R., Zhang, C., Wang, J., Hou, J., Zhao, J., Shi, L., Beom-Seok, P., Choi, S.R., Lim, Y.P., Meng, J. 2007. Flowering time quantitative trait loci analysis of oilseed brassica in multiple environments and genomewide alignment with arabidopsis. Genetics 17(4): 2433-2444.
  • Miller, J.C., Tanksley, S.D. 1989. RFLP analysis of polygenetic relationships and genetic variation in the genus lycopersicon. Theor. Appl. Genet. 80: 437–448.
  • Mohammadi, S.A. Prassanna, B.M. 2003. Analysis of genetic diversity in crop plants-salient statistical tools and considerations, Crop Sci. 43: 1235–1248.
  • Nsabiyera, V., Logose, M., Ochwo-Ssemakula, M., Sseruwagi, P., Gibson, P., Ojiewo, C. 2013. Morphological characterization of local and exotic hot pepper (Capsicum annuum L.) collections in Uganda. Biorem. Biodiv Bioavail 7(1): 22-32.
  • Papa, R., Gepts, P. 2003. Asymmetry of gene flow and differential geographical structure of molecular diversity in wild and domesticated common bean (Phaseolus vulgaris L.) from mesoamerica. Theor. Appl. Genet. 106(2): 239-250.
  • Smith, J.S.C., Smith, O.S. 1989. The description and assessment of distances between ınbred lines of maize: the utility of morphological, biochemical and genetic descriptors and a scheme for the testing of distinctiveness between ınbred lines. Maydica 34: 151-161.
  • Souza, E., Sorrells, M.E. 1991. Genetic relationships among 70 north american oat cultivars, ı: cluster analysis using quantitative morphological characters. Crop Sci. 31: 599-605.
  • Toquica S. P., Rodriguez, F., Martinez, E., Duque, M.C., Tohme, J. 2003. Molecular characterization by aflps of capsicum germplasm from the amazon department in colombia, characterization by AFLPs of Capsicum. Genet. Resour. Crop Evol. 50: 639–647.
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Bölüm Tarımsal Biyoteknoloji
Yazarlar

M.Kadri Bozkalfa

Tansel Kaygısız Aşcıoğlu

Dursun Eşiyok

Yayımlanma Tarihi 15 Ekim 2017
Kabul Tarihi 29 Eylül 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 32 Sayı: 3

Kaynak Göster

APA Bozkalfa, M., Kaygısız Aşcıoğlu, T., & Eşiyok, D. (2017). Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 32(3), 321-329. https://doi.org/10.7161/omuanajas.284511
AMA Bozkalfa M, Kaygısız Aşcıoğlu T, Eşiyok D. Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi. ANAJAS. Ekim 2017;32(3):321-329. doi:10.7161/omuanajas.284511
Chicago Bozkalfa, M.Kadri, Tansel Kaygısız Aşcıoğlu, ve Dursun Eşiyok. “Biber Genotiplerinin Genetik çeşitliliklerinin Srap markörleri kullanılarak Belirlenmesi”. Anadolu Tarım Bilimleri Dergisi 32, sy. 3 (Ekim 2017): 321-29. https://doi.org/10.7161/omuanajas.284511.
EndNote Bozkalfa M, Kaygısız Aşcıoğlu T, Eşiyok D (01 Ekim 2017) Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi. Anadolu Tarım Bilimleri Dergisi 32 3 321–329.
IEEE M. Bozkalfa, T. Kaygısız Aşcıoğlu, ve D. Eşiyok, “Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi”, ANAJAS, c. 32, sy. 3, ss. 321–329, 2017, doi: 10.7161/omuanajas.284511.
ISNAD Bozkalfa, M.Kadri vd. “Biber Genotiplerinin Genetik çeşitliliklerinin Srap markörleri kullanılarak Belirlenmesi”. Anadolu Tarım Bilimleri Dergisi 32/3 (Ekim 2017), 321-329. https://doi.org/10.7161/omuanajas.284511.
JAMA Bozkalfa M, Kaygısız Aşcıoğlu T, Eşiyok D. Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi. ANAJAS. 2017;32:321–329.
MLA Bozkalfa, M.Kadri vd. “Biber Genotiplerinin Genetik çeşitliliklerinin Srap markörleri kullanılarak Belirlenmesi”. Anadolu Tarım Bilimleri Dergisi, c. 32, sy. 3, 2017, ss. 321-9, doi:10.7161/omuanajas.284511.
Vancouver Bozkalfa M, Kaygısız Aşcıoğlu T, Eşiyok D. Biber genotiplerinin genetik çeşitliliklerinin srap markörleri kullanılarak belirlenmesi. ANAJAS. 2017;32(3):321-9.
Online ISSN: 1308-8769