Effects of different growth regulators on regeneration of Turkish upland rice

Year 2018, Volume: 33 Issue: 3, 226 - 231, 18.10.2018

Yilmaz Kaya Selin Karakütük

https://doi.org/10.7161/omuanajas.407998

Cited By: 5

Abstract

Rice is a staple crop grown in the world especially in the
Asian region. The rice faces with different biotics and abiotic stress factors,
especially drought stress. Turkish upland rice is one of the special rice types
grown on drought stress condition, having a large potential value. Our aim was
to analyse the effects of different concentrations of BAP+NAA and KIN+2,4-D
combination  on rice grown in vitro condition. For this purpose,
mature seeds of Turkish upland rice were selected as starting material for
direct/indirect regeneration response in serial treatment. The best medium for
induction of shoot regeneration was MS₁ supplemented with 1.5 mg L^-1
BAP and 0.5 NAA mg L^-1. 
The results showed that the maximum callus formation (65.54 %) was obtained
on MS medium containing 2.4-D (2.0 mg L^-1) within 21 days from
nodes. With this study, we have developed a plant tissue culture for Turkish
upland rice, suggesting positive methods for rice biotechnology.

Rice is a staple crop grown in the world especially in the
Asian region. The rice faces with different biotics and abiotic stress factors,
especially drought stress. Turkish upland rice is one of the special rice types
grown on drought stress condition, having a large potential value. Our aim was
to analyse the effects of different concentrations of BAP+NAA and KIN+2,4-D
combination  on rice grown in vitro condition. For this purpose,
mature seeds of Turkish upland rice were selected as starting material for
direct/indirect regeneration response in serial treatment. The best medium for
induction of shoot regeneration was MS₁ supplemented with 1.5 mg L^-1
BAP and 0.5 NAA mg L^-1. 
The results showed that the maximum callus formation (65.54 %) was obtained
on MS medium containing 2.4-D (2.0 mg L^-1) within 21 days from
nodes. With this study, we have developed a plant tissue culture for Turkish
upland rice, suggesting positive methods for rice biotechnology.

Keywords

Kallus, Bitki rejenerasyonu, BAP, NAA, KIN, 2.4-D

Farklı büyüme düzenleyicilerin Türk kır çeltiği rejenerasyonuna etkisi

Abstract

Çeltik bitkisi, tüm Dünya’da ve özellikle Asya
kıtasında yetişen temel bir üründür. Çeltik, kuraklık stresi gibi farklı
abiyotik ve biyotik stres faktörleri ile karşı karşıyadır. Türk kır çeltiği,
kuraklık stres koşullarında yetişen ve büyük bir potansiyele sahip olan üstün
çeltik türlerinden biridir. Bu çalışmanın amacı, BAP + NAA ve KIN + 2.4-D
kombinasyonunun farklı konsantrasyonlardaki in vitro koşullarda yetiştirilen
çeltik üzerindeki etkilerini incelemektir. Bu amaçla, başlangıç materyali
olarak olgun çeltik tohumları kullanılmış olup direkt ve indirekt rejenerasyon
sonuçları kaydedilmiştir. Gövde rejenerasyonu indüksiyonu için en iyi ortam,
1.5 mg L^-1 BAP ve 0.5 NAA mg L^-1 ile desteklenmiş MS₁
olmuştur. Sonuç olarak, gövde nodların 21 gün içinde 2.4-D (2.0 mg L^-1)
içeren MS ortamı üzerinde maksimum kallus oluşumunun (% 65.54) elde edildiğini
kaydedilmiştir. Bu çalışma ile Türk kır çeltiği için doku kültürü optimizasyonu
geliştirirmiştir ve çeltik biyoteknolojisi açısından pozitif metotlar
sunulmuştur.

Keywords

Callus, Plant regeneration, BAP, NAA, KIN, 2.4-DIN

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