Effects of different growth regulators on regeneration of Turkish upland rice

Yıl 2018, Cilt: 33 Sayı: 3, 226 - 231, 18.10.2018

Yilmaz Kaya , Selin Karakütük

https://doi.org/10.7161/omuanajas.407998

Cited By: 5

Öz

Rice is a staple crop grown in the world especially in the
Asian region. The rice faces with different biotics and abiotic stress factors,
especially drought stress. Turkish upland rice is one of the special rice types
grown on drought stress condition, having a large potential value. Our aim was
to analyse the effects of different concentrations of BAP+NAA and KIN+2,4-D
combination  on rice grown in vitro condition. For this purpose,
mature seeds of Turkish upland rice were selected as starting material for
direct/indirect regeneration response in serial treatment. The best medium for
induction of shoot regeneration was MS₁ supplemented with 1.5 mg L^-1
BAP and 0.5 NAA mg L^-1. 
The results showed that the maximum callus formation (65.54 %) was obtained
on MS medium containing 2.4-D (2.0 mg L^-1) within 21 days from
nodes. With this study, we have developed a plant tissue culture for Turkish
upland rice, suggesting positive methods for rice biotechnology.

Rice is a staple crop grown in the world especially in the
Asian region. The rice faces with different biotics and abiotic stress factors,
especially drought stress. Turkish upland rice is one of the special rice types
grown on drought stress condition, having a large potential value. Our aim was
to analyse the effects of different concentrations of BAP+NAA and KIN+2,4-D
combination  on rice grown in vitro condition. For this purpose,
mature seeds of Turkish upland rice were selected as starting material for
direct/indirect regeneration response in serial treatment. The best medium for
induction of shoot regeneration was MS₁ supplemented with 1.5 mg L^-1
BAP and 0.5 NAA mg L^-1. 
The results showed that the maximum callus formation (65.54 %) was obtained
on MS medium containing 2.4-D (2.0 mg L^-1) within 21 days from
nodes. With this study, we have developed a plant tissue culture for Turkish
upland rice, suggesting positive methods for rice biotechnology.

Anahtar Kelimeler

Kallus, Bitki rejenerasyonu, BAP, NAA, KIN, 2.4-D

Farklı büyüme düzenleyicilerin Türk kır çeltiği rejenerasyonuna etkisi

Öz

Çeltik bitkisi, tüm Dünya’da ve özellikle Asya
kıtasında yetişen temel bir üründür. Çeltik, kuraklık stresi gibi farklı
abiyotik ve biyotik stres faktörleri ile karşı karşıyadır. Türk kır çeltiği,
kuraklık stres koşullarında yetişen ve büyük bir potansiyele sahip olan üstün
çeltik türlerinden biridir. Bu çalışmanın amacı, BAP + NAA ve KIN + 2.4-D
kombinasyonunun farklı konsantrasyonlardaki in vitro koşullarda yetiştirilen
çeltik üzerindeki etkilerini incelemektir. Bu amaçla, başlangıç materyali
olarak olgun çeltik tohumları kullanılmış olup direkt ve indirekt rejenerasyon
sonuçları kaydedilmiştir. Gövde rejenerasyonu indüksiyonu için en iyi ortam,
1.5 mg L^-1 BAP ve 0.5 NAA mg L^-1 ile desteklenmiş MS₁
olmuştur. Sonuç olarak, gövde nodların 21 gün içinde 2.4-D (2.0 mg L^-1)
içeren MS ortamı üzerinde maksimum kallus oluşumunun (% 65.54) elde edildiğini
kaydedilmiştir. Bu çalışma ile Türk kır çeltiği için doku kültürü optimizasyonu
geliştirirmiştir ve çeltik biyoteknolojisi açısından pozitif metotlar
sunulmuştur.

Anahtar Kelimeler

Callus, Plant regeneration, BAP, NAA, KIN, 2.4-DIN

Kaynakça

• Ali, S., Xue, Q., Zhang, X., 2004. Assessment of various factors involved in the tissue culture system in rice. Rice Sci. 11:345–349.
• Bouman, BAM., Yang, X., Wang, H., Wang, Z., Zhao, J., Chen, B., 2006. Performance of aerobic rice cultivars under irrigated conditions in North China. Field Crops Research. 9, 53-65. https://doi.org/10.1016/j.fcr.2005.08.015
• Din Mohd, ARJ., Iliyas, AF., Wagiran, A., Abd Samad, A., Rahmat, Z., Sarmidi, MR., 2016. Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas). Saudi Journal of Biological Sciences. 23(1), pp. 69–77. http://doi.org/10.1016/j.sjbs.2015.10.022
• Geng, PP., La, HG., Wang, HQ., Stevens EJC., 2008. Effect of sorbitol concentration on regeneration of embryogenic callus in upland rice varieties (Oryza sativa L.). Plant Cell Tissue Organ Cult., 92:, 303–313. https://doi.org/10.1007/s11240-007-9321-5
• Horuz, A., Korkmaz, A., 2014. Çeltikte (Oryza sativa L.) Tuz stresinin azaltılmasında silisyumlu gübrelemenin etkisi. Tarım Bilimleri Dergisi, 20, 215-229.
• Jahan, N., Norshila, F., Javed, MA., Samiullah, K., Siti, Z., 2015. Effects of ferrous toxicity on seedling traits and Ion Distribution pattern in Upland and Low Land Rice under hydroponic conditions. Jurnal Teknologi, eISSN 2180–3722 |DOI: 10.11113/jt.v78.7258.
• Jahan, N., Javed MA., Manan, FA., Khan, S., Samad AA., Zaidi, MI0., 2017. Comparative study of physiological and growth parameters for sodium and iron toxicities in upland and lowland cultivars of Oryza sativa L. Pak. J. Bot., 49(SI), 249-254.
• Binti, JA., 2015. Effect of plant growth regulators in plant regeneration of Malaysian Upland Rice. Master Thesis, Universiti Teknologi Malaysia. P. 40-44
• Ahmad, JF., Khan, MS., 2012. Reproducible and expedient rice regeneration system using in vitro grown plants. African Journal of Biotechnology, 11(1), 138-144. http://dx.doi.org/10.5897/AJB11.2521
• Karthikeyan A., Pandian STK, Ramesh, M., 2009. High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.). Phys. Mol. Biol. Plants, 15, 371–375. https://doi.org/10.1007/s12298-009-0042-6
• Karakütük, S., 2017. Türkiye’de yetiştirilen kır çeltik çeşitlerinin in vitro koşullarda doku kültürü parametrelerinin ve kurakliğa toleranslarinin belirlenmesi. Yüksek Lisans Tezi. Ondokuz Mayıs Üniversitesi. Samsun. S. 50-55
• Kaya, Y., Marakli, S., Gozikirmizi, N., Mohamed, E., Javed, MA., Huyop, F., 2013. Herbicide tolerance genes derived from bacteria. The J. Anim. Plant Sci., 23(1): 85–91.
• Kaya, Y., Kuyumcu, G., Karakütük, S., Arvas YE., 2017. Kır çeltik bitkisi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 27 (1), 151-156. doi: 10.29133/yyutbd.305114
• Kurt, O., Aydın, E., Seyis, F., 2008. Farklı somatik explantların çeltikte (Oryza sativa L. cv. Taipei-309) kallus ve Bitkicik Oluşumuna Etkisi. Biyoloji Bilimler Araştırma Dergisi, 1(1):1-3.
• Kurt, O., 2015. Hücre ve doku kültürü. Bitki ıslahı ders kitabı, No: 43 (5. Basım). Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi. Samsun.
• Kurt, S., 2016. Çeltik (Oryza sativa L.)’te somatik dokulardan kallus oluşumu ve bitki elde etme Olanakları,. Yüksek Lisans Tezi. Ondokuz Mayıs Üniversitesi, Samsun. S.60-63.
• Ramesh, M., Murugiah, V., Gupta AK., 2009. Efficient in vitro plant regeneration via leaf base segments of indica rice, Ind. J. Exp. Biol., 47. 68–74
• Rueb, S., Leneman, M., Schilperoor, R., Hensgens, A., 1994. Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell Tissue and Organ Culture, 36, 259-264. https://doi.org/10.1007/BF00037729
• Kumar, SS., Ajinder, K., Jagdeep, SS., 2014. High frequency embryogenic callus induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake. J Rice Res. 2, 2. http://dx.doi.org/10.4172/jrr.1000125
• Sahoo, KK., Tripathi, AK., Pareek, A., Sopory, SK., Singla-Pareek, SL., 2011. An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars. Plant Methods, 7, 49-59. DOI: 10.1186/1746-4811-7-49
• Shekar, SRS., Singh, B., 2016. Optimization of regeneration using differential growth regulators in indica rice cultivars. 3 Biotech. 6(19). pp. 2-7. ISSN: 2190-572X (Print) 2190-5738 (Online).
• Shahsavari, E., 2010. Evaluation and optimizations of media on the tissue culture system of upland rice. Int. J. Agric. Biol., 12, 537–540. Doi.org/10–177/MMI/2010/12–4–537–540.
• Shahsavari, E., 2011. Impact of tryptophan and glutamine on tissue culture of upland rice. Plant Soil Environ., 57, 7–10.
• Shahsavari, E., Maheran, AA., Siti, Nor, Akmar, A., Hanafi, MM., 2010. The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice. Afr. J. Biotechnol, 9, 2089–2094. DOI: 10.5897/AJB10.1925.
• Yang, C., Yang, L., Yang, Y., Ouyang, Z., 2004. Rice root growth and nutrient uptake as influenced by organic manure in continuously and alternately flooded paddy soils. Agricultural Water Management, 70, 67–81.
• Zhao, W., Zheng, S., Ling, H., 2011. An regeneration system and Agrobacterium-mediated transformation of Chinese upland rice cultivar Handao297. Plant Cell Tissue Organ Cult., 106, 475–483.
• Wang, LG., Lin, DZ., Wang, JC., 2011. Tissue culture system for different hybrid of Indica Rice. Journal of Northeast Agricultural University, 18(2), pp. 13-17. https://doi.org/10.1016/S1006-8104(12)60003-8