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Farklı Güçteki MS Besin Ortamlarının Spathiphyllum'un in Vitro Çoğaltımı ve Köklenmesi Üzerine Etkileri

Year 2022, Volume: 37 Issue: 3, 583 - 592, 31.10.2022
https://doi.org/10.7161/omuanajas.1082219

Abstract

Spathiphyllum Araceae familyasına ait bir iç mekan süs bitkisidir. Doğada 36 türü bulunan Spathiphyllum cinsinin doğada 30 türü bulunmaktadır. Spathiphyllum çok güçlü büyüyen ve bakımı kolay iç mekân bitkisidir. Spathiphyllum'un vejetatif çoğaltımının zor olduğu bilinmektedir. Bu nedenle mevcut üretim talebi yeterince karşılayamamaktadır. Artış gösteren spathiphyllum ihtiyacını karşılamak için klasik üretim yöntemleri ile bitki doku kültürü yöntemleri de kullanılmaktadır. Bu çalışmada, spathiphyllum'un mikroçoğaltımı ve köklenmesi üzerine MS besin ortamlarının farklı kuvvetlerinin (tam, ½ ve ¼) etkilerinin belirlenmesi amaçlanmıştır. Sürgün uçları, mikroçoğaltım için 1 mg L-1 BAP eklenmiş farklı kuvvetteki MS ortamlarında kültüre alınmıştır. Mikroçoğaltımdan gelen bitkicikler, köklenme için 1 mg L-1 IBA ilave edilmiş farklı güçteki MS ortamlarına aktarılmıştır. Köklü bitkiler kontrollü sera koşullarına alıştırılmıştır. Mikroçoğaltımda tüm parametrelerde ortamın en iyi gücü tam MS olarak tespit edilmiştir. Köklenme oranı her ortam için %100 olarak belirlenmiştir. Ancak kök sayısı, kök uzunluğu açısından ortamlar arasında önemli farklılıklar tespit edilmiştir.

References

  • Bandyopadhyay, T.K., Bandyopadhyay, M., da Silva, J.A.T., Paul, S., Dam, A., Ghosh, P.D., 2011. An efficient micropropagation protocol to control abnormality in longterm shoot cultures of Spathiphyllum floribundum (L.) ‘Petite’. Floriculture and Ornamental Biotechnology, 5(1): 57-63.
  • Bringslimark, T., Hartig, T., Patil, G.G., 2009. The psychological benefits of indoor plants: A critical review of the experimental literature. Journal of Environmental Psychology, 29(4): 422-433. doi: 10.1016/j.jenvp.2009.05.001
  • Das, A., Paul, A.K., Chaudhuri, S., 2000. Micropropagation of Spathiphyllum wallisii-an important ornamental plant. Horticultural Journal, 13(2): 71-75.
  • Dewir, Y.H., Chakrabarty, D., Hahn, E.J., Paek, K.Y., 2006. A simple method for mass propagation of Spathiphyllum cannifolium using an airlift bioreactor. In Vitro Cellular Developmental Biology – Plant, 42: 291–297. https://doi.org/10.1079/IVP2006764
  • Dönmez, D., 2022. Regeneration of plants from alginate-encapsulated shoot tips of myrtle (Myrtus communis L.). Erwerbs-Obstbau, 1-8. doi: 10.1007/s10341-021-00632-z
  • Dönmez, D., Şimşek, Ö., Kaçar, Y.A., 2016. Genetic engineering techniques in fruit science. International Journal of Environmental and Agriculture Research, 2(12): 115-128.
  • Fan, S., Mao, F., Liu, B., Jian, D., Li, X., Zuo, X., 2019. Tissue culture and rapid propagation of Spathiphyllum kochii Engl, et Krause. Agricultural Biotechnology, 8(2): 86-89.
  • Geneyikli, E., 2009. Researches on micropropogation for ‘barış zambağı’ (spathiphyllum) varieties. MSc. Thesis, Department of Biotechnology, Institute of Natural and Applied Sciences, University of Çukurova, Turkey.
  • Hilae, A., Te-chato, S., 2005. Effects of carbon sources and strength of MS medium on germination of somatic embryos of oil palm (Elaeis quineensis Jacq.). Songklanakarin Journal of Science and Technology, 27(3): 629-635.
  • Ibrahim, S.M., Hashish, K.I., Taha, L.S., Mazhar, A.A., Kandil, M.M., 2016. In vitro culture protocol, micropropagation, acclimatization and chemical constituents of Spathiphyllum cannifolium plant under copper concentration effect. International Journal of PharmTech Research, 9(12): 33-41.
  • Kaçar, Y.A., Dönmez, D., Biçen, B., Erol, M.H., Şimsek, Ö., Mendi, Y.Y., 2020. Micropropagation of spathiphyllum with temporary immersion bioreactor system. Turkish Journal of Agriculture-Food Science and Technology, 8(5): 1195-1200. doi: 10.24925/turjaf.v8i5.1195-1200.3364
  • Kaçar, Y.A., Mendi, Y.Y., Cetiner, S., Serce, S., 2005. The effect of cytokinin type and concentration on multiplication rate of spathiphyllum (fam. Araceae). Asian Journal of Plant Sciences, 4(4): 401-404.
  • Kurtuluş, O., Dönmez, D., Biçen, B., Şimşek, Ö., Çimen, B., Yeşiloğlu, T., Küden, A. Aka Kaçar, Y., 2021. Investigation of Micropropagation and Rooting Performances of Some Citrus Hybrids in in vitro Conditions. Yuzuncu Yil University Journal of Agricultural Sciences, 31 (1): 19-29. doi: 10.29133/yyutbd.810784
  • Lee, E.J., Paek, K.Y., 2012. Enhanced productivity of biomass and bioactive compounds through bioreactor cultures of Eleutherococcus koreanum Nakai adventitious roots affected by medium salt strength. Industrial crops and products, 36(1): 460-465. doi: 10.1016/j.indcrop.2011.10.033
  • Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
  • Ramirez-Malagon, R., Borodanenko, A., Barrera-Guerra, J.L., Ochoa-Alejo, N., 2001. Shoot number and shoot size as affected by growth regulators in in vitro cultures of Spathiphyllum floribundum L. Scientia Horticulturae, 89(3): 227-236. doi: 10.1016/S0304-4238(00)00236-3
  • Rezali, N.I., Sidik, N.J., Saleh, A., Osman, N.I., Adam, N.A.M., 2017. The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme. Asian Pacific Journal of Tropical Biomedicine, 7(2): 151-156. doi:10.1016/j.apjtb.2016.11.019
  • Richardson, E.A., Pearce, J., Mitchell, R., Kingham, S., 2013. Role of physical activity in the relationship between urban green space and health. Public health, 127(4): 318-324. doi: 10.1016/j.puhe.2013.01.004
  • Sevindik, B., Tutuncu, M., Izgu, T., Tagipur, E.M., Curuk, P., Yilmaz, O., ... Mendi, Y.Y., 2017. Micropropagation of Erodium olympicum endemic to Turkey. American Journal of Plant Biology, 2(3-1): 24-27. doi: 10.11648/j.ajpb.s.2017020501.14
  • Şimsek, Ö., Dönmez, D., Aka Kaçar, Y., 2018. Investigation into performance of some citrus rootstocks in in vitro drought stress conditions. Yuzuncu Yil University Journal of Agricultural Sciences, 28(3): 305-310. doi: 10.29133/yyutbd.428140
  • Şimşek, Ö., Biçen, B., Dönmez, D., Kaçar, Y.A., 2017. Effects of different media on micropropagation and rooting of Myrtle (Myrtus communis L.) in in vitro conditions. International Journal of Environmental and Agriculture Research, 3(10): 54-59.
  • Teixeira da Silva, J.A., Giang, D.D.T., Tanaka, M., 2006. Photoautotrophic micropropagation of spathiphyllum. Photosynthetica, 44(1): 53-61. doi: 10.1007/s11099-005-0158-z
  • Tütüncü, M., Özcan, M., Mendi, Y.Y., 2019. Bazı siklamen türlerinde farklı doku kültürü ortamlarının gynogenesis üzerine etkileri. Anadolu Tarım Bilimleri Dergisi, 34: 239-249.
  • Yazici, K., 2020. Importance of Indoor Ornamental Plants and Determination of Consumer Trends: A Case Study in Tokat City. Journal of Bartin Faculty of Forestry, 22(3): 738-747. doi: 10.24011/barofd.743195

The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum

Year 2022, Volume: 37 Issue: 3, 583 - 592, 31.10.2022
https://doi.org/10.7161/omuanajas.1082219

Abstract

The Spathiphyllum is an indoor ornamental plant belonging to the Araceae family. Spathiphyllum genus, which has 36 species in nature, is a robust growth and easy to care for an indoor plant. Vegetative propagation of spathiphyllum is known to be complicated. For this reason, current production does not meet the demand sufficiently. Today, plant tissue culture methods and classical production methods are used to meet the increasing demand for spathiphyllum. In the present study, we aimed to detect the effects of different strengths (full, ½, and ¼) of MS media on micropropagation and rooting of spathiphyllum. Shoot tips of spathiphyllum ‘chico’ variety were cultured in different strengths of MS media containing 1 mg L-1 BAP for micropropagation. Plantlets obtained from micropropagation were transferred to different strengths of MS media containing 1 mg L-1 IBA for rooting. Plantlets in magenta boxes were removed and replaced in a controlled greenhouse. The best strength of medium was detected as the full MS in all parameters in vitro propagation. The rooting rate was determined 100% for each medium. However, there are significant differences among the media in view of the number of roots and length of roots.

References

  • Bandyopadhyay, T.K., Bandyopadhyay, M., da Silva, J.A.T., Paul, S., Dam, A., Ghosh, P.D., 2011. An efficient micropropagation protocol to control abnormality in longterm shoot cultures of Spathiphyllum floribundum (L.) ‘Petite’. Floriculture and Ornamental Biotechnology, 5(1): 57-63.
  • Bringslimark, T., Hartig, T., Patil, G.G., 2009. The psychological benefits of indoor plants: A critical review of the experimental literature. Journal of Environmental Psychology, 29(4): 422-433. doi: 10.1016/j.jenvp.2009.05.001
  • Das, A., Paul, A.K., Chaudhuri, S., 2000. Micropropagation of Spathiphyllum wallisii-an important ornamental plant. Horticultural Journal, 13(2): 71-75.
  • Dewir, Y.H., Chakrabarty, D., Hahn, E.J., Paek, K.Y., 2006. A simple method for mass propagation of Spathiphyllum cannifolium using an airlift bioreactor. In Vitro Cellular Developmental Biology – Plant, 42: 291–297. https://doi.org/10.1079/IVP2006764
  • Dönmez, D., 2022. Regeneration of plants from alginate-encapsulated shoot tips of myrtle (Myrtus communis L.). Erwerbs-Obstbau, 1-8. doi: 10.1007/s10341-021-00632-z
  • Dönmez, D., Şimşek, Ö., Kaçar, Y.A., 2016. Genetic engineering techniques in fruit science. International Journal of Environmental and Agriculture Research, 2(12): 115-128.
  • Fan, S., Mao, F., Liu, B., Jian, D., Li, X., Zuo, X., 2019. Tissue culture and rapid propagation of Spathiphyllum kochii Engl, et Krause. Agricultural Biotechnology, 8(2): 86-89.
  • Geneyikli, E., 2009. Researches on micropropogation for ‘barış zambağı’ (spathiphyllum) varieties. MSc. Thesis, Department of Biotechnology, Institute of Natural and Applied Sciences, University of Çukurova, Turkey.
  • Hilae, A., Te-chato, S., 2005. Effects of carbon sources and strength of MS medium on germination of somatic embryos of oil palm (Elaeis quineensis Jacq.). Songklanakarin Journal of Science and Technology, 27(3): 629-635.
  • Ibrahim, S.M., Hashish, K.I., Taha, L.S., Mazhar, A.A., Kandil, M.M., 2016. In vitro culture protocol, micropropagation, acclimatization and chemical constituents of Spathiphyllum cannifolium plant under copper concentration effect. International Journal of PharmTech Research, 9(12): 33-41.
  • Kaçar, Y.A., Dönmez, D., Biçen, B., Erol, M.H., Şimsek, Ö., Mendi, Y.Y., 2020. Micropropagation of spathiphyllum with temporary immersion bioreactor system. Turkish Journal of Agriculture-Food Science and Technology, 8(5): 1195-1200. doi: 10.24925/turjaf.v8i5.1195-1200.3364
  • Kaçar, Y.A., Mendi, Y.Y., Cetiner, S., Serce, S., 2005. The effect of cytokinin type and concentration on multiplication rate of spathiphyllum (fam. Araceae). Asian Journal of Plant Sciences, 4(4): 401-404.
  • Kurtuluş, O., Dönmez, D., Biçen, B., Şimşek, Ö., Çimen, B., Yeşiloğlu, T., Küden, A. Aka Kaçar, Y., 2021. Investigation of Micropropagation and Rooting Performances of Some Citrus Hybrids in in vitro Conditions. Yuzuncu Yil University Journal of Agricultural Sciences, 31 (1): 19-29. doi: 10.29133/yyutbd.810784
  • Lee, E.J., Paek, K.Y., 2012. Enhanced productivity of biomass and bioactive compounds through bioreactor cultures of Eleutherococcus koreanum Nakai adventitious roots affected by medium salt strength. Industrial crops and products, 36(1): 460-465. doi: 10.1016/j.indcrop.2011.10.033
  • Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
  • Ramirez-Malagon, R., Borodanenko, A., Barrera-Guerra, J.L., Ochoa-Alejo, N., 2001. Shoot number and shoot size as affected by growth regulators in in vitro cultures of Spathiphyllum floribundum L. Scientia Horticulturae, 89(3): 227-236. doi: 10.1016/S0304-4238(00)00236-3
  • Rezali, N.I., Sidik, N.J., Saleh, A., Osman, N.I., Adam, N.A.M., 2017. The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme. Asian Pacific Journal of Tropical Biomedicine, 7(2): 151-156. doi:10.1016/j.apjtb.2016.11.019
  • Richardson, E.A., Pearce, J., Mitchell, R., Kingham, S., 2013. Role of physical activity in the relationship between urban green space and health. Public health, 127(4): 318-324. doi: 10.1016/j.puhe.2013.01.004
  • Sevindik, B., Tutuncu, M., Izgu, T., Tagipur, E.M., Curuk, P., Yilmaz, O., ... Mendi, Y.Y., 2017. Micropropagation of Erodium olympicum endemic to Turkey. American Journal of Plant Biology, 2(3-1): 24-27. doi: 10.11648/j.ajpb.s.2017020501.14
  • Şimsek, Ö., Dönmez, D., Aka Kaçar, Y., 2018. Investigation into performance of some citrus rootstocks in in vitro drought stress conditions. Yuzuncu Yil University Journal of Agricultural Sciences, 28(3): 305-310. doi: 10.29133/yyutbd.428140
  • Şimşek, Ö., Biçen, B., Dönmez, D., Kaçar, Y.A., 2017. Effects of different media on micropropagation and rooting of Myrtle (Myrtus communis L.) in in vitro conditions. International Journal of Environmental and Agriculture Research, 3(10): 54-59.
  • Teixeira da Silva, J.A., Giang, D.D.T., Tanaka, M., 2006. Photoautotrophic micropropagation of spathiphyllum. Photosynthetica, 44(1): 53-61. doi: 10.1007/s11099-005-0158-z
  • Tütüncü, M., Özcan, M., Mendi, Y.Y., 2019. Bazı siklamen türlerinde farklı doku kültürü ortamlarının gynogenesis üzerine etkileri. Anadolu Tarım Bilimleri Dergisi, 34: 239-249.
  • Yazici, K., 2020. Importance of Indoor Ornamental Plants and Determination of Consumer Trends: A Case Study in Tokat City. Journal of Bartin Faculty of Forestry, 22(3): 738-747. doi: 10.24011/barofd.743195
There are 24 citations in total.

Details

Primary Language English
Journal Section Anadolu Tarım Bilimleri Dergisi
Authors

Dicle Dönmez 0000-0002-7446-9405

Mansur Hakan Erol 0000-0003-2424-5670

Belgin Biçen 0000-0001-8931-4759

Özhan Şimşek 0000-0001-5552-095X

Yıldız Aka Kaçar 0000-0001-5314-7952

Early Pub Date October 27, 2022
Publication Date October 31, 2022
Acceptance Date October 6, 2022
Published in Issue Year 2022 Volume: 37 Issue: 3

Cite

APA Dönmez, D., Hakan Erol, M., Biçen, B., Şimşek, Ö., et al. (2022). The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum. Anadolu Tarım Bilimleri Dergisi, 37(3), 583-592. https://doi.org/10.7161/omuanajas.1082219
AMA Dönmez D, Hakan Erol M, Biçen B, Şimşek Ö, Aka Kaçar Y. The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum. ANAJAS. October 2022;37(3):583-592. doi:10.7161/omuanajas.1082219
Chicago Dönmez, Dicle, Mansur Hakan Erol, Belgin Biçen, Özhan Şimşek, and Yıldız Aka Kaçar. “The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum”. Anadolu Tarım Bilimleri Dergisi 37, no. 3 (October 2022): 583-92. https://doi.org/10.7161/omuanajas.1082219.
EndNote Dönmez D, Hakan Erol M, Biçen B, Şimşek Ö, Aka Kaçar Y (October 1, 2022) The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum. Anadolu Tarım Bilimleri Dergisi 37 3 583–592.
IEEE D. Dönmez, M. Hakan Erol, B. Biçen, Ö. Şimşek, and Y. Aka Kaçar, “The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum”, ANAJAS, vol. 37, no. 3, pp. 583–592, 2022, doi: 10.7161/omuanajas.1082219.
ISNAD Dönmez, Dicle et al. “The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum”. Anadolu Tarım Bilimleri Dergisi 37/3 (October 2022), 583-592. https://doi.org/10.7161/omuanajas.1082219.
JAMA Dönmez D, Hakan Erol M, Biçen B, Şimşek Ö, Aka Kaçar Y. The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum. ANAJAS. 2022;37:583–592.
MLA Dönmez, Dicle et al. “The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum”. Anadolu Tarım Bilimleri Dergisi, vol. 37, no. 3, 2022, pp. 583-92, doi:10.7161/omuanajas.1082219.
Vancouver Dönmez D, Hakan Erol M, Biçen B, Şimşek Ö, Aka Kaçar Y. The Effects of Different Strength of MS Media on in Vitro Propagation and Rooting of Spathiphyllum. ANAJAS. 2022;37(3):583-92.
Online ISSN: 1308-8769