ELİT KAYAKLI KOŞUCULARDA PPAR-A'NIN (RS4253778) SERUM LİPİTLERİ ÜZERİNDEKİ ETKİSİNİN İNCELENMESİ

Abstract

Bu çalışmanın amacı, elit kayaklı koşucularda PPAR-a (rs4253778) serum lipidleri üzerindeki etkilerini incelemektir. Bu çalışmaya 34 kayaklı koşu sporcusu (Türkiye kayak milli takım kampına katılan 23 erkek ve 11 kadın) dahil edildi. PPAR-a geni rs4253778 G/C polimorfizmi için genotipleme, Tercyk multicanal amplifikatör ve restriksiyon enzim sindirimi üzerinde PCR ile gerçekleştirildi. İstatistiksel analiz SPSS 22.0 paket programı kullanılarak yapıldı. Kayaklı koşucularda PPAR-a polimorfizminin genotipik frekansı tespit edildi. 34 kayaklı koşucuda PPAR-a GG, GC ve CC genotipleri sırasıyla %67,64, %23,52 ve % 8,82 tespit edildi. PPAR-a GG, GC ve CC genotipleri erkeklerde sırasıyla %47,05, %16,64 ve %2,94 olarak tespit edilirken kadınlarda PPAR-a GG, GC ve CC genotipleri sırasıyla %20,58, %5,88 ve %5,88 olarak tespit edildi. PPAR-a G ve C alleli 34 elit dayanıklılık sporcusunda sırasıyla 49 ve 19 olarak tespit edildi. Bu çalışmada, GG genotipleri elit sporcularda (sırasıyla %67,64) GC ve CC'den (sırasıyla %23,52 ve %8,82) daha yüksek frekanslarda olduğu tespit edildi. Türk elit kros kayakçılarının PPAR-a G/C gen polimorfizmi ile serum toplam kolesterol, HDL-kolesterol, LDL-kolesterol ve TG seviyeleri arasındaki istatistiksel olarak anlamlı bir fark tespit edilmedi. Türk elit kayaklı koşucuların PPAR-a G/C gen polimorfizmi ile lipid profilleri arasında istatistiksel olarak anlamlı bir fark olmamasına rağmen, PPAR-α geninin dayanıklılık gerektiren sporlarda dayanıklılık performansı üzerinde önemli bir etkiye sahip olduğu öngörülmektedir.

Keywords

• Kayaklı koşucular
• Lipitler
• Spor
• PPAR-A gen poliformizmi

THE EXAMINATION OF THE EFFECTS OF PPAR-A (RS4253778) ON SERUM LIPIDS IN ELITE CROSS-COUNTRY SKIERS

Abstract

The purpose of this study was to examine the effects of PPAR-a (rs4253778) on serum lipids in elite cross-country skiers. This study included 34 cross-country skiers (23 males and 11 females who participated in the Turkish skiing national team camp). Genotyping for the PPAR-a gene rs4253778 G/C polymorphism was performed by PCR on Tercyk multicanal amplificator and restriction enzyme digestion. Statistical analysis was done by using the SPSS 22.0 package program. Genotypic frequency of the PPAR-a polymorphism was detected in cross-country skiers. PPAR-a GG, GC and CC genotypes were detected as 67.64%, 23.52% and 8.82%, respectively in 34 cross-country skiers while PPAR-a GG, GC and CC genotypes were detected as 47.05%, 16.64%, and 2.94%, respectively in elite males. PPAR-a GG, GC and CC genotypes were detected as 20.58 %, 5.88%, and 5.88%, respectively in females. PPAR-a G and C allele were detected as 49 and 19, respectively in 34 elite endurance athletes. In the present study, the GG genotypes were detected at higher frequencies in elite athletes (67.64% respectively) than GC and CC (23.52% and 8.82%, respectively). The difference between the PPAR-a G/C gene polymorphism of Turkish elite cross-country skiers and serum total cholesterol, HDL- cholesterol, LDL- cholesterol and TG levels was not statistically significant. Although there was not any statistically significant difference between the PPAR-a G/C gene polymorphism and lipid profiles of Turkish elite cross-country skiers, it is foreseen that PPAR-α genes have an important effect on endurance performance in sports requiring endurance such as cross-country skiing.

Keywords

• Cross-Country athletes
• Lipids
• PPAR-A Gene polymorphism
• Sports

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