ELİT KAYAKLI KOŞUCULARDA PPAR-A'NIN (RS4253778) SERUM LİPİTLERİ ÜZERİNDEKİ ETKİSİNİN İNCELENMESİ

Öz

Bu çalışmanın amacı, elit kayaklı koşucularda PPAR-a (rs4253778) serum lipidleri üzerindeki etkilerini incelemektir. Bu çalışmaya 34 kayaklı koşu sporcusu (Türkiye kayak milli takım kampına katılan 23 erkek ve 11 kadın) dahil edildi. PPAR-a geni rs4253778 G/C polimorfizmi için genotipleme, Tercyk multicanal amplifikatör ve restriksiyon enzim sindirimi üzerinde PCR ile gerçekleştirildi. İstatistiksel analiz SPSS 22.0 paket programı kullanılarak yapıldı. Kayaklı koşucularda PPAR-a polimorfizminin genotipik frekansı tespit edildi. 34 kayaklı koşucuda PPAR-a GG, GC ve CC genotipleri sırasıyla %67,64, %23,52 ve % 8,82 tespit edildi. PPAR-a GG, GC ve CC genotipleri erkeklerde sırasıyla %47,05, %16,64 ve %2,94 olarak tespit edilirken kadınlarda PPAR-a GG, GC ve CC genotipleri sırasıyla %20,58, %5,88 ve %5,88 olarak tespit edildi. PPAR-a G ve C alleli 34 elit dayanıklılık sporcusunda sırasıyla 49 ve 19 olarak tespit edildi. Bu çalışmada, GG genotipleri elit sporcularda (sırasıyla %67,64) GC ve CC'den (sırasıyla %23,52 ve %8,82) daha yüksek frekanslarda olduğu tespit edildi. Türk elit kros kayakçılarının PPAR-a G/C gen polimorfizmi ile serum toplam kolesterol, HDL-kolesterol, LDL-kolesterol ve TG seviyeleri arasındaki istatistiksel olarak anlamlı bir fark tespit edilmedi. Türk elit kayaklı koşucuların PPAR-a G/C gen polimorfizmi ile lipid profilleri arasında istatistiksel olarak anlamlı bir fark olmamasına rağmen, PPAR-α geninin dayanıklılık gerektiren sporlarda dayanıklılık performansı üzerinde önemli bir etkiye sahip olduğu öngörülmektedir.

Anahtar Kelimeler

• Kayaklı koşucular
• Lipitler
• Spor
• PPAR-A gen poliformizmi

THE EXAMINATION OF THE EFFECTS OF PPAR-A (RS4253778) ON SERUM LIPIDS IN ELITE CROSS-COUNTRY SKIERS

Öz

The purpose of this study was to examine the effects of PPAR-a (rs4253778) on serum lipids in elite cross-country skiers. This study included 34 cross-country skiers (23 males and 11 females who participated in the Turkish skiing national team camp). Genotyping for the PPAR-a gene rs4253778 G/C polymorphism was performed by PCR on Tercyk multicanal amplificator and restriction enzyme digestion. Statistical analysis was done by using the SPSS 22.0 package program. Genotypic frequency of the PPAR-a polymorphism was detected in cross-country skiers. PPAR-a GG, GC and CC genotypes were detected as 67.64%, 23.52% and 8.82%, respectively in 34 cross-country skiers while PPAR-a GG, GC and CC genotypes were detected as 47.05%, 16.64%, and 2.94%, respectively in elite males. PPAR-a GG, GC and CC genotypes were detected as 20.58 %, 5.88%, and 5.88%, respectively in females. PPAR-a G and C allele were detected as 49 and 19, respectively in 34 elite endurance athletes. In the present study, the GG genotypes were detected at higher frequencies in elite athletes (67.64% respectively) than GC and CC (23.52% and 8.82%, respectively). The difference between the PPAR-a G/C gene polymorphism of Turkish elite cross-country skiers and serum total cholesterol, HDL- cholesterol, LDL- cholesterol and TG levels was not statistically significant. Although there was not any statistically significant difference between the PPAR-a G/C gene polymorphism and lipid profiles of Turkish elite cross-country skiers, it is foreseen that PPAR-α genes have an important effect on endurance performance in sports requiring endurance such as cross-country skiing.

Anahtar Kelimeler

• Cross-Country athletes
• Lipids
• PPAR-A Gene polymorphism
• Sports

Kaynakça

1. Ahmetov, I.I., Mozhayskaya, I.A., Flavell, D.M., Astratenkova, I.V., Komkova, A.I., Lyubaeva, E.V., Tarakin, P.P., Shenkman, B.S., Vdovina, A.B., Netreba, A.I., Popov, D.V., Vinogradova, O.L., Montgomery, H.E., Rogozkin, V.A. (2006). PPARalpha gene variation and physical performance in Russian athletes. European Journal of Applied Physiology, 97(1), 103–108. doi: 10.1007/s00421-006-0154-4
2. Ahmetov, I., Egorova, E.S., Mustafina, L.J. (2013). The PPARa gene polymorphism in team sports athletes. Central European Journal of Sport Sciences and Medicine, 1, 19-24.
3. Amir, O., Amir, R., Yamin, C., Attias, E., Eynon, N., Sagiv, M., Sagiv, M., Meckel, Y. (2007). The ACE deletion allele is associated with Israeli elite endurance athletes. Experimental Physiology, 92(5), 881–886. doi: 10.1113/expphysiol.2007.038711
4. Barjaktarović-Labović S., Andrejević V., Banjari I., Kurgaš H., Zejnilović M. (2015). Lipid Status of Professıonal Athletes. MD-Medical Data, 7(1), 21-25.
5. Berger, J., Moller, D. E. (2002). The mechanisms of action of PPARs. Annual Review of Medicine, 53, 409–435. doi: 10.1146/annurev.med.53.082901.104018
6. Bray, M.S., Hagberg, J.M., Pérusse, L., Rankinen, T., Roth, S.M., Wolfarth, B., Bouchard, C. (2009). The human gene map for performance and health-related fitness phenotypes: the 2006-2007 update. Medicine and Science in Sports and Exercise, 41(1), 35–73. doi:10.1249/mss.0b013e3181844179
7. Corak, A., Kapici, S., Sercan, C., Akkoç, O., Ulucan, K. (2017). A pilot study for determination of anxiety related SLC6A4 promoter "S" and "L" alleles in healthy Turkish athletes. Cellular and Molecular Biology (Noisy-le-Grand, France), 63(5), 29–31. doi: 10.14715/cmb/2017.63.5.6
8. Eynon, N., Meckel, Y., Sagiv, M., Yamin, C., Amir, R., Sagiv, M., Goldhammer, E., Duarte, J.A., Oliveira, J. (2010). Do PPARGC1A and PPARalpha polymorphisms influence sprint or endurance phenotypes?. Scandinavian Journal of Medicine & Science in Sports, 20(1), e145–e150. doi: 10.1111/j.1600-0838.2009.00930.x

9. Flavell, Jamshidi, Hawe, Torra, Taskinen, Frick, Nieminen, Kesäniemi, Pasternack, Staels, Miller, Humphries, Talmud, Syvänne. (2002). Peroxisome proliferator-activated receptor α gene variants influence progression of coronary atherosclerosis and risk of coronary artery disease. Circulation, 105, 1440–1445. doi: 10.1161/01.cir.0000012145.80593.25
10. Hautala, A.J., Leon, A.S., Skinner, J.S., Rao, D.C., Bouchard, C., Rankinen, T. (2007). Peroxisome proliferator-activated receptor-delta polymorphisms are associated with physical performance and plasma lipids: the HERITAGE Family Study. American journal of physiology. Heart and Circulatory Physiology, 292(5), H2498–H2505. doi: 10.1152/ajpheart.01092.2006
11. Kaynar Ö., Öztürk N., Kıyıcı F., Baygutalp N.K., Bakan E. (2016). The effects of short-term intensive exercise on levels of liver enzymes and serum lipids in kick boxing athletes. Dicle Medical Journal, 43(1),130-134. doi: 10.5798/diclemedj.0921.2016.01.0652
12. Kaynar, Ö, Bilici, M. (2017). Analysis of the Talent Selection in Turkish Wrestling. International Journal of Sport Culture and Science, 5 (4), 347-355. doi: 10.14486/IntJSCS698
13. Krämer, D.K., Ahlsén, M., Norrbom, J., Jansson, E., Hjeltnes, N., Gustafsson, T., Krook, A. (2006). Human skeletal muscle fibre type variations correlate with PPAR alpha, PPAR delta and PGC-1 alpha mRNA. Acta Physiologica (Oxford, England), 188(3-4), 207–216. doi: 10.1111/j.1748-1716.2006.01620.x
14. Liang, H., & Ward, W. F. (2006). PGC-1alpha: a key regulator of energy metabolism. Advances in Physiology Education, 30(4), 145–151. doi: 10.1152/advan.00052.2006
15. Lopez-Leon, S., Tuvblad, C., Forero, D.A. (2016). Sports genetics: the PPARA gene and athletes' high ability in endurance sports. A systematic review and meta-analysis. Biology of Sport, 33(1), 3–6. doi: 10.5604/20831862.1180170
16. Lucia, A., Gómez-Gallego, F., Barroso, I., Rabadán, M., Bandrés, F., San Juan, A.F., Chicharro, J. L., Ekelund, U., Brage, S., Earnest, C.P., Wareham, N. J., Franks, P. W. (2005). PPARGC1A genotype (Gly482Ser) predicts exceptional endurance capacity in European men. Journal of Applied Physiology (Bethesda, Md. : 1985), 99(1), 344–348. doi: 10.1152/japplphysiol.00037.2005.
17. Macarthur, D.G., North, K.N. (2005). Genes and human elite athletic performance. Human Genetics, 116(5), 331–339. doi: 10.1007/s00439-005-1261-8
18. Maciejewska, A., Sawczuk, M., Cięszczyk, P. (2011). Variation in the PPARα gene in Polish rowers. Journal of Science and Medicine in Sport, 14(1), 58–64. doi: 10.1016/j.jsams.2010.05.006
19. Mazzotti, D.R., Singulane, C.C., Ota, V.K., Rodrigues, T.P., Furuya, T.K., de Souza, F.J., Cordeiro, B.G., Magalhães, C., Chen, E.S., Jacomini, A., Smith, M., Borsatto-Galera, B. (2011). PPARα polymorphisms as risk factors for dyslipidemia in a Brazilian population. Molecular Genetics and Metabolism, 102(2), 189–193. doi: 10.1016/j.ymgme.2010.11.156
20. Petr, M., Stastny, P., Pecha, O., Šteffl, M., Šeda, O., Kohlíková, E. (2015). Correction: PPARA Intron Polymorphism Associated with Power Performance in 30-s Anaerobic Wingate Test. PloS one, 10(7), e0134424. doi: 10.1371/journal.pone.0107171
21. Rankinen, T., Bray, M. S., Hagberg, J. M., Pérusse, L., Roth, S. M., Wolfarth, B., Bouchard, C. (2006). The human gene map for performance and health-related fitness phenotypes: the 2005 update. Medicine and Science in Sports and Exercise, 38(11), 1863–1888. doi: 10.1249/01.mss.0000233789.01164.4f
22. Saritaş N. (2012). Effect of endurance exercise training on blood lipids in young men. African Journal of Pharmacy and Pharmacology, 6(3), 216-220.
23. Silva, D., Arend, E., Rocha, S. M., Rudnitskaya, A., Delgado, L., Moreira, A., & Carvalho, J. (2019). The impact of exercise training on the lipid peroxidation metabolomic profile and respiratory infection risk in older adults. European Journal of Sport Science, 19(3), 384–393. doi: 10.1080/17461391.2018.1499809 Epub 2018 Jul 23.
24. Sung, Y.C., Liao, Y. H., Chen, C.Y., Chen, Y.L., Chou, C. C. (2017). Acute changes in blood lipid profiles and metabolic risk factors in collegiate elite taekwondo athletes after short-term de-training: a prospective insight for athletic health management. Lipids in Health and Disease, 16(1), 143. doi: 10.1186/s12944-017-0534-2
25. Ulucan, K., Sercan, C., Biyikli, T. (2015). Distribution of Angiotensin-1 Converting Enzyme Insertion/Deletion and α-Actinin-3 Codon 577 Polymorphisms in Turkish Male Soccer Players. Genetics & Epigenetics, 7, 1–4. doi: 10.4137/GEG.S31479. eCollection 2015.
26. van Raalte, D.H., Li, M., Pritchard, P. H., Wasan, K. M. (2004). Peroxisome proliferator-activated receptor (PPAR)-alpha: a pharmacological target with a promising future. Pharmaceutical Research, 21(9), 1531–1538. doi:10.1023/b:pham.0000041444.06122.8d
27. Wang Y, & Xu D. (2017). Effects of aerobic exercise on lipids and lipoproteins. Lipids in Health and Disease, 16(1), 132. doi: 10.1186/s12944-017-0515-5
28. Yessoufou, A., & Wahli, W. (2010). Multifaceted roles of peroxisome proliferator-activated receptors (PPARs) at the cellular and whole organism levels. Swiss Medical Weekly, 140, w13071. doi: 10.4414/smw.2010.13071
29. Yong, E.L., Li, J., Liu, M.H. (2008). Single gene contributions: genetic variants of peroxisome proliferator-activated receptor (isoforms alpha, beta/delta and gamma) and mechanisms of dyslipidemias. Current Opinion in Lipidology, 19(2), 106–112. doi: 10.1097/MOL.0b013e3282f64542