Effect of Iturin A on Oxidative Stress and Immune Response in Breast Cancer Cell Lines

Öz

This study aimed to evaluate the effects of Iturin A on oxidative stress and DNA damage, and immune response in MCF-7 and MDA-MB-231 breast cancer cells. Total antioxidant capacity (TAC), total oxidant status (TOS), and 8-hydroxy-2′-deoxiguanosine (8-OHdG) levels were determined by spectrophotometric and ELISA methods, oxidative stress index (OSI) was calculated. NF-κB and COX-2 gene expression levels were analyzed using RT-qPCR. Iturin A significantly increased TAC (p<0.0001 in both), TOS (p<0.0001 in both), and 8-OHdG (p<0.01 and p<0.001, respectively) levels in both MCF-7 and MDA-MB-231 cells. Iturin A treatment significantly increased OSI levels in MCF-7 cells (p<0.001), although an increase in MDA-MB-231 cells was not statistically significant (p>0.05). Iturin A treatment significantly reduced NF-κB expression in MCF-7 cells (p<0.0001) and MDA-MB-231 (p<0.05) cells. But, the observed changes in COX-2 gene expression were not statistically significant in either cell line (p>0.05). Iturin A may differentially modulate oxidative balance and immune response in estrogen receptor-positive and -negative breast cancer cells. These findings suggest that Iturin A could be a potential agent targeting redox and inflammation balance in breast cancer therapy.

Anahtar Kelimeler

• Iturin A
• oxidative stress
• breast cancer
• MCF-7
• MDA-MB-231

İturin A’nın Meme Kanseri Hücre Hatlarında Oksidatif Stres Üzerine Etkisi

Öz

Bu çalışma, Iturin A’nın MCF-7 ve MDA-MB-231 meme kanseri hücrelerinde oksidatif stres DNA hasarı ve immün yanıt etkilerini değerlendirmek amacıyla yapılmıştır. Hücrelerde toplam antioksidan kapasite (TAK), toplam oksidan statüsü (TOS) ve 8-hidroksi-2′-deoksiguanozin (8-OHdG) seviyeleri spektrofotometre ve ELISA yöntemleriyle ölçülmüş ve oksidatif stres indeksi (OSI) hesaplanmıştır. NF-κB ve COX-2 gen ekspresyon düzeyleri RT-qPCR yöntemiyle analiz edilmiştir. Iturin A, hem MCF-7 hücrelerinde hem de MDA-MB-231 hücrelerinde. TAK (her ikisinde de p<0,0001), TOS (her ikisinde de p<0,0001) ve 8-OHdG (sırasıyla, p<0,01 ve p<0,001) düzeylerini önemli ölçüde artırmıştır. Iturin A, MCF-7 hücrelerinde OSI (p<0,001) düzeylerinde istatistiksel olarak anlamlı artışa yol açarken, MDA-MB-231 hücrelerinde istatistiksel olarak anlamlı artışa neden olmamıştır (p>0,05). Iturin A tedavisi, MCF-7 (p<0,0001) ve MDA-MB-231 (p<0,05) hücrelerinde NF-κB ekspresyonunu önemli ölçüde azaltmıştır. Ancak, COX-2 gen ekspresyonunda gözlenen değişimler, her iki hücre hattında da istatistiksel olarak anlamlı bulunmamıştır (p>0,05). Iturin A, östrojen reseptörü pozitif ve negatif meme kanseri hücrelerinde oksidatif denge ve immün yanıtı farklı biçimlerde modüle edebilir. Bu bulgular, Iturin A’nın meme kanseri tedavisinde redoks ve inflamasyon dengesini hedef alan potansiyel bir ajan olabileceğini göstermektedir.

Anahtar Kelimeler

• Iturin A
• Oksidatif stres
• Meme kanseri
• MCF-7
• MDA-MB-231

Kaynakça

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