Mirtazapin Diyabet Oluşturulan Sıçanların Beyinciklerindeki Hasarı Kısmen Düzeltmektedir

Öz

Diabetes mellitus sistemik bir hastalık olup çeşitli organlarda hasara neden olmaktadır. Bu organlar arasında beyincik de yer almaktadır. Mirtazapin ise majör depresyon tedavisinde kullanılan bir antidepresan madde olup inflamasyon yanıtının düzenlenmesinde de rol oynamaktadır.  Bütün bu bilgiler ışığında, çalışmamızda DM oluşturulan sıçanların beyinciklerinde meydana gelen değişiklikleri ve bu değişiklikler üzerine mirtazapinin etkilerini araştırmayı amaçladık. Çalışmamızda toplam 21 adet yetişkin, erkek Sprague Dawley sıçan 3 eşit gruba ayrıldı (n=7). Kontrol grubuna hiçbir uygulama yapılmadı. DM grubundaki hayvanlara tek doz 55 mg/kg streptozotosin intraperitoneal olarak verilerek DM oluşturuldu. Mirtazapin grubuna streptozotosin uygulamasından 28 gün sonra, 14 gün boyunca günde 20 mg/kg mirtazapin gavaj yoluyla uygulandı. Deney sonunda alınan beyincik örnekleri %10’luk formaldehit ile fikse edildikten sonra rutin doku takip işleminin ardından alınan kesitlere hematoksilen ve eozin, krezil viyole boyama teknikleri ve akson rejenerasyonunu belirlemek amacıyla da GAP-43 immünohistokimyasal boyaması uygulandı. Kontrol grubunda beyincikte normal histoloji ile medüllada yüksek GAP-43 ekspresyonu gözlendi. DM grubunda Purkinje nöronlarında büzüşme ve bazı alanlarda hücre ölümü saptanırken, orta düzeyde GAP-43 immünoreaksiyonu belirlendi. Mirtazapin uygulanan grupta ise Purkinje nöronlarının ve histolojik yapının kontrol grubuna yakın olduğu gözlenirken GAP-43 ekspresyonunun DM grubuna benzer olduğu saptandı. Sonuç olarak, streptozotosinle oluşturulan DM sıçanların beyinciğinde özellikle Purkinje nöronlarında hasara ve medüllada miyelin kaybına yol açmakta ve mirtazapin uygulaması Purkinje nöronlarındaki hasarı azaltmakta, ancak miyelin rejenerasyonunu sağlamada belirgin bir etki göstermemektedir.

Anahtar Kelimeler

• Diabetes mellitus,Serebellum,Mirtazapin,GAP43

Mirtazapine Partially Ameliorate Damage of the Diabetic Rat Cerebellum

Öz

Diabetes mellitus is a systemic disease that causes damage to various organs. Among these organs, cerebellum is also present. Mirtazapine is an antidepressant used in the treatment of major depression also regulating the inflammatory response. With all this information, we aimed to investigate the effects of DM on rat cerebellum and effects of mirtazapine on DM-induced cerebellum damage. In our study 21 adult, male, Sprague Dawley rats were equaly divided into 3 groups (n = 7). No treatment was made to the control group. Animals in the DM group received a single dose of 55 mg/kg streptozotocin intraperitoneally. Mirtazapine was administered via gavage 20 mg / kg mirtazapine daily for 14 days after 28 days of streptozotocin administration. After 28 days of streptozotin administration 20 mg / kg mirtazapine was administered to the mirtazapine group via gavage per day for 14 days. At the end of the experiment, cerebellum specimens were fixed with 10% formaldehyde, following routine tissue processing hematoxylin-eosin, krezil violet dyeing techniques and GAP-43 immunohistochemical staining made to determine axonal regeneration. The control group had high GAP-43 expression in medullary with normal histology in the cerebellum. Moderate GAP-43 immunoreactivity, cell death in some areas and shrinkage of Purkinje neurons was detected in the DM group. In the mirtazapine group, Purkinje neurons were found to protect normal structures relatively better, while GAP-43 expression was found to be similar to the DM group. As a result, streptozotocin-induced DM leads to loss of myelin in the brain, especially in Purkinje neurons damage, and mirtazapine administration, which reduces damage in the Purkinje neurons, but does not have a significant effect on myelin regeneration.

Anahtar Kelimeler

• Diabetes mellitus,Cerebellum,Mirtazapine

Kaynakça

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